LOCUS       sequence-167205-        1042 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  2nd Pro5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Tan CY, Hirakawa H, Suzuki R, Haga T, Iwata F, Nagamune T
  TITLE     Immobilization of a Bacterial Cytochrome P450 Monooxygenase System 
            on a Solid Support.
  JOURNAL   Angew Chem Int Ed Engl. 2016 Oct 26. doi: 10.1002/anie.201608033.
  PUBMED    27781345
REFERENCE   2  (bases 1 to 1042)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Angew Chem 
            Int Ed Engl. 2016 Oct 26. doi: 10.1002/anie.201608033."
COMMENT     Sequence Label: pHPro52
FEATURES             Location/Qualifiers
     source          1..1042
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    323..344
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(359..364,365..382,383..389)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    397..413
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     402..424
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     421..437
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     421..437
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(523..540)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(523..539)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(532..554)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(694..711)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(945..964)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtgctgatgc cgctggcgat tcaggttcat catgccgttt gtgatggctt ccatgtcggc
       61 agaatgctta atgaattaca acagtactgc gatgagtggc agggcggggc gtaatttttt
      121 taaggcagtt attggtgccc ttaaacgcct ggttgctacg cctgaataag tgataataag
      181 cggatgaatg gcagaaattc agcttggccc agtgccaagc tccaatacgc aaaccgcctc
      241 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
      301 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
      361 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
      421 caggaaacag ctatgaccat gattacgaat tggtctcgac cgcctcctcc accgccacga
      481 gaccgatcct ctagagtcga cctgcaggca tgcaagcttg gcactggccg tcgttttaca
      541 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
      601 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
      661 cagcctgaat ggcgaatgag cttcttccgc ttcctcgctc actgactcgc tgcgctcggt
      721 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
      781 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
      841 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
      901 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
      961 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     1021 gtccgccttt ctcccttcgg ga
//
