LOCUS       sequence-167206-       11466 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  target  miRNA168  for destruction in Arabidopsis thaliana.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11466)
  AUTHORS   Tang G, Yan J, Gu Y, Qiao M, Fan R, Mao Y, Tang X
  TITLE     Construction of short tandem target mimic (STTM) to block the 
            functions of plant and animal microRNAs.
  JOURNAL   Methods. 2012 Oct;58(2):118-25. doi: 10.1016/j.ymeth.2012.10.006. 
            Epub 2012 Oct 23.
  PUBMED    23098881
REFERENCE   2  (bases 1 to 11466)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ymeth.2012.10.006"; journalName: "Methods"; date: 
            "2012-10"; volume: "58"; issue: "2"; pages: "118-25"
COMMENT     Sequence Label: pFGC5941-PacI-ath-STTM168
FEATURES             Location/Qualifiers
     source          1..11466
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1214..1232)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1215..1232)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     1871..1890
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(1993..1998,1999..2015,2016..2021))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     promoter        2296..2676
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     CDS             2682..3233
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     terminator      3243..3495
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     misc_feature    3581..3605
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             4030..4824
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      4911..5499
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5653..5670
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    5685..5825
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(5751..5770)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5911..5933
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      6169..6363
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(6429..7502)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(7931..8560)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    9861..9885
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     10073..10095
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10087..10104
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10088..10104
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(10129..10836)
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     primer_bind     11015..11034
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        11015..11033
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        join(11441..11466,1..320)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
ORIGIN
        1 cgggaaacct cctcggattc cattgcccag ctatctgtca cctcatcgaa aggacagtag
       61 aaaaggaagg tggctcctac aaatgccatc attgcgataa aggaaaggct atcattcaag
      121 atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa
      181 aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgacatc tccactgaca
      241 taagggatga cgcacaaccc cactaacctt cgcaagaccc ttcctctata taaggaagtt
      301 catttcattt ggagaggaca gcccaagctt ttcccgatct gctacaccaa gcgagttgtt
      361 gttgttatgg tctaattttg gtctaaagaa gaagaatttc ccgatctgct acaccaagcg
      421 agaattcggt acgctgaaat caccagtctc tctctacaaa tctatctctc tctattttct
      481 ccataaataa tgtgtgagta gtttcccgat aagggaaatt agggttctta tagggtttcg
      541 ctcatgtgtt gagcatataa gaaaccctta gtatgtattt gtatttgtaa aatacttcta
      601 tcaataaaat ttctaattcc taaaaccaaa atccagtact aaaatccgga tctcctaaag
      661 tccctataga tctttgtcgt gaatataaac cagacacgag acgactagac ctggagccca
      721 gacgccgttc gaagctagaa gtaccgctta ggcaggaggc cgttagggaa aagatgctaa
      781 ggcagggttg gttacgttga ctcccccgta ggtttggttt aaatatgatg aagtggacgg
      841 aaggaaggag gaagacaagg aaggataagg ttgcaggccc tgtgcaaggt aagaagatgg
      901 gaatttgata gaggtacgct actatactta tactatacgc taagggaatg attgtattta
      961 taccctatac cccctagtaa ccccttatca atttaagaaa taatccgcat aagcccccgc
     1021 ttaaaaattg gtatcagagc catgaatagg tctatgacca aaactcaaga ggataaaacc
     1081 tcaccaaaat acgaaagggt ccttaactct aaagataaaa gatctttcaa gatcaaaact
     1141 agttccctca caccggtgac ggggatcgca tgcgacctct agaccatcac attggagctt
     1201 cagctgctcg agttctatag tgtcacctaa atcgtatgtg tatgatacat aaggttatgt
     1261 attaattgta gccgcgttct tacgtcgacg taccaattac gccccgccct gtcactcatc
     1321 gcagtactgt tgtaattcat taagcattct gccgacatgg aagccatcac aaacggcatg
     1381 atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat atttgcccat
     1441 ggtgaaaacg ggggcgaaga agttgtccat attggccacg tttaaatcaa aactggtgaa
     1501 actcacccag ggattggctg agacgaaaaa catattctca ataaaccctt tagggaaata
     1561 ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa actgccggaa
     1621 atcgtcgtgg tattcactcc agagcgatga aaacgtttca gtttgctcat ggaaaacggt
     1681 gtaacaaggg tgaacactat cccatattac cagctcaccg tctttcattg ccatacggaa
     1741 ctccggatga gcattcatca ggcgggcaag aatgtgaata aaggccggat aaaacttgtg
     1801 cttatttttc tttacggtct ttaaaaaggc cgtaatatcc agccgaacgg tctggttata
     1861 ggtacattga gcaactgact gaaatgcctc aaaatgttct ttacgatgcc attgggatat
     1921 atcaacggtg gtatatccag tgatttcttt ctccatttta gcttccttag cccctgaaaa
     1981 tctcgatgac tcattaaagc ttatcgatga taagctgtca aacatgagaa tttagcccgc
     2041 ctaatgagcg ggctttttgt tgtgatccgc gttgctggcg tttttccata ggctccgccc
     2101 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     2161 ataaagatac caggcgtttc ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     2221 tgccgcttac cggatacctg tccgcctttc ttaattaact ctctagactc acctaggatc
     2281 catgaattct gagatttttc aaatcagtgc gcaagacgtg acgtaagtat ccgagtcagt
     2341 ttttattttt ctactaattt ggtcgtttat ttcggcgtgt aggacatggc aaccgggcct
     2401 gaatttcgcg ggtattctgt ttctattcca actttttctt gatccgcagc cattaacgac
     2461 ttttgaatag atacgctgac acgccaagcc tcgctagtca aaagtgtacc aaacaacgct
     2521 ttacagcaag aacggaatgc gcgtgacgct cgcggtgacg ccatttcgcc ttttcagaaa
     2581 tggataaata gccttgcttc ctattatatc ttcccaaatt accaatacat tacactagca
     2641 tctgaatttc ataaccaatc tcgatacacc aaatcgaatc gatgagccca gaacgacgcc
     2701 cggccgacat ccgccgtgcc accgaggcgg acatgccggc ggtctgcacc atcgtcaacc
     2761 actacatcga gacaagcacg gtcaacttcc gtaccgagcc gcaggaaccg caggagtgga
     2821 cggacgacct cgtccgtctg cgggagcgct atccctggct cgtcgccgag gtggacggcg
     2881 aggtcgccgg catcgcctac gcgggcccct ggaaggcacg caacgcctac gactggacgg
     2941 ccgagtcgac cgtgtacgtc tccccccgcc accagcggac gggactgggc tccacgctct
     3001 acacccacct gctgaagtcc ctggaggcac agggcttcaa gagcgtggtc gctgtcatcg
     3061 ggctgcccaa cgacccgagc gtgcgcatgc acgaggcgct cggatatgcc ccccgcggca
     3121 tgctgcgggc ggccggcttc aagcacggga actggcatga cgtgggtttc tggcagctgg
     3181 acttcagcct gccggtaccg ccccgtccgg tcctgcccgt caccgagatt tgactgcaga
     3241 atcttggact cccatgttgg caaaggcaac caaacaaaca atgaatgatc cgctcctgca
     3301 tatggggcgg tttgagtatt tcaactgcca tttgggctga attgaagaca tgctcctgtc
     3361 agaaattccg tgatcttact caatattcag taatctcggc caatatccta aatgtgcgtg
     3421 gctttatctg tctttgtatt gtttcatcaa ttcatgtaac gtttgctttt cttatgaatt
     3481 ttcaaataaa ttatcagatc cgagctcgaa ttaattcggc gttaattcag tacattaaaa
     3541 acgtccgcaa tgtgttatta agttgtctaa gcgtcaattt gtttacacca caatatatcc
     3601 tgccaccagc cagccaacag ctccccgacc ggcagctcgg cacaaaatca ccactcgata
     3661 caggcagccc atcagtccgg gacggcgtca gcgggagagc cgttgtaagg cggcagactt
     3721 tgctcatgtt accgatgcta ttcggaagaa cggcaactaa gctgccgggt ttgaaacacg
     3781 gatgatctcg cggagggtag catgttgatt gtaacgatga cagagcgttg ctgcctgtga
     3841 tcaccgcggt ttcaaaatcg gctccgtcga tactatgtta tacgccaact ttgaaaacaa
     3901 ctttgaaaaa gctgttttct ggtatttaag gttttagaat gcaaggaaca gtgaattgga
     3961 gttcgtcttg ttataattag cttcttgggg tatctttaaa tactgtagaa aagaggaagg
     4021 aaataataaa tggctaaaat gagaatatca ccggaattga aaaaactgat cgaaaaatac
     4081 cgctgcgtaa aagatacgga aggaatgtct cctgctaagg tatataagct ggtgggagaa
     4141 aatgaaaacc tatatttaaa aatgacggac agccggtata aagggaccac ctatgatgtg
     4201 gaacgggaaa aggacatgat gctatggctg gaaggaaagc tgcctgttcc aaaggtcctg
     4261 cactttgaac ggcatgatgg ctggagcaat ctgctcatga gtgaggccga tggcgtcctt
     4321 tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga ttatcgagct gtatgcggag
     4381 tgcatcaggc tctttcactc catcgacata tcggattgtc cctatacgaa tagcttagac
     4441 agccgcttag ccgaattgga ttacttactg aataacgatc tggccgatgt ggattgcgaa
     4501 aactgggaag aagacactcc atttaaagat ccgcgcgagc tgtatgattt tttaaagacg
     4561 gaaaagcccg aagaggaact tgtcttttcc cacggcgacc tgggagacag caacatcttt
     4621 gtgaaagatg gcaaagtaag tggctttatt gatcttggga gaagcggcag ggcggacaag
     4681 tggtatgaca ttgccttctg cgtccggtcg atcagggagg atatcgggga agaacagtat
     4741 gtcgagctat tttttgactt actggggatc aagcctgatt gggagaaaat aaaatattat
     4801 attttactgg atgaattgtt ttagtaccta gaatgcatga ccaaaatccc ttaacgtgag
     4861 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     4921 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     4981 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     5041 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
     5101 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     5161 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     5221 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     5281 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     5341 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     5401 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     5461 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     5521 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     5581 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     5641 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt
     5701 ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc
     5761 tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg
     5821 ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
     5881 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
     5941 cgtcatcacc gaaacgcgcg aggcagggtg ccttgatgtg ggcgccggcg gtcgagtggc
     6001 gacggcgcgg cttgtccgcg ccctggtaga ttgcctggcc gtaggccagc catttttgag
     6061 cggccagcgg ccgcgatagg ccgacgcgaa gcggcggggc gtagggagcg cagcgaccga
     6121 agggtaggcg ctttttgcag ctcttcggct gtgcgctggc cagacagtta tgcacaggcc
     6181 aggcgggttt taagagtttt aataagtttt aaagagtttt aggcggaaaa atcgcctttt
     6241 ttctctttta tatcagtcac ttacatgtgt gaccggttcc caatgtacgg ctttgggttc
     6301 ccaatgtacg ggttccggtt cccaatgtac ggctttgggt tcccaatgta cgtgctatcc
     6361 acaggaaaga gaccttttcg acctttttcc cctgctaggg caatttgccc tagcatctgc
     6421 tccgtacatt aggaaccggc ggatgcttcg ccctcgatca ggttgcggta gcgcatgact
     6481 aggatcgggc cagcctgccc cgcctcctcc ttcaaatcgt actccggcag gtcatttgac
     6541 ccgatcagct tgcgcacggt gaaacagaac ttcttgaact ctccggcgct gccactgcgt
     6601 tcgtagatcg tcttgaacaa ccatctggct tctgccttgc ctgcggcgcg gcgtgccagg
     6661 cggtagagaa aacggccgat gccgggatcg atcaaaaagt aatcggggtg aaccgtcagc
     6721 acgtccgggt tcttgccttc tgtgatctcg cggtacatcc aatcagctag ctcgatctcg
     6781 atgtactccg gccgcccggt ttcgctcttt acgatcttgt agcggctaat caaggcttca
     6841 ccctcggata ccgtcaccag gcggccgttc ttggccttct tcgtacgctg catggcaacg
     6901 tgcgtggtgt ttaaccgaat gcaggtttct accaggtcgt ctttctgctt tccgccatcg
     6961 gctcgccggc agaacttgag tacgtccgca acgtgtggac ggaacacgcg gccgggcttg
     7021 tctcccttcc cttcccggta tcggttcatg gattcggtta gatgggaaac cgccatcagt
     7081 accaggtcgt aatcccacac actggccatg ccggccggcc ctgcggaaac ctctacgtgc
     7141 ccgtctggaa gctcgtagcg gatcacctcg ccagctcgtc ggtcacgctt cgacagacgg
     7201 aaaacggcca cgtccatgat gctgcgacta tcgcgggtgc ccacgtcata gagcatcgga
     7261 acgaaaaaat ctggttgctc gtcgcccttg ggcggcttcc taatcgacgg cgcaccggct
     7321 gccggcggtt gccgggattc tttgcggatt cgatcagcgg ccgcttgcca cgattcaccg
     7381 gggcgtgctt ctgcctcgat gcgttgccgc tgggcggcct gcgcggcctt caacttctcc
     7441 accaggtcat cacccagcgc cgcgccgatt tgtaccgggc cggatggttt gcgaccgctc
     7501 acgccgattc ctcgggcttg ggggttccag tgccattgca gggccggcag gcaacccagc
     7561 cgcttacgcc tggccaaccg cccgttcctc cacacatggg gcattccacg gcgtcggtgc
     7621 ctggttgttc ttgattttcc atgccgcctc ctttagccgc taaaattcat ctactcattt
     7681 attcatttgc tcatttactc tggtagctgc gcgatgtatt cagatagcag ctcggtaatg
     7741 gtcttgcctt ggcgtaccgc gtacatcttc agcttggtgt gatcctccgc cggcaactga
     7801 aagttgaccc gcttcatggc tggcgtgtct gccaggctgg ccaacgttgc agccttgctg
     7861 ctgcgtgcgc tcggacggcc ggcacttagc gtgtttgtgc ttttgctcat tttctcttta
     7921 cctcattaac tcaaatgagt tttgatttaa tttcagcggc cagcgcctgg acctcgcggg
     7981 cagcgtcgcc ctcgggttct gattcaagaa cggttgtgcc ggcggcggca gtgcctgggt
     8041 agctcacgcg ctgcgtgata cgggactcaa gaatgggcag ctcgtacccg gccagcgcct
     8101 cggcaacctc accgccgatg cgcgtgcctt tgatcgcccg cgacacgaca aaggccgctt
     8161 gtagccttcc atccgtgacc tcaatgcgct gcttaaccag ctccaccagg tcggcggtgg
     8221 cccatatgtc gtaagggctt ggctgcaccg gaatcagcac gaagtcggct gccttgatcg
     8281 cggacacagc caagtccgcc gcctggggcg ctccgtcgat cactacgaag tcgcgccggc
     8341 cgatggcctt cacgtcgcgg tcaatcgtcg ggcggtcgat gccgacaacg gttagcggtt
     8401 gatcttcccg cacggccgcc caatcgcggg cactgccctg gggatcggaa tcgactaaca
     8461 gaacatcggc cccggcgagt tgcagggcgc gggctagatg ggttgcgatg gtcgtcttgc
     8521 ctgacccgcc tttctggtta agtacagcga taaccttcat gcgttcccct tgcgtatttg
     8581 tttatttact catcgcatca tatacgcagc gaccgcatga cgcaagctgt tttactcaaa
     8641 tacacatcac ctttttagac ggcggcgctc ggtttcttca gcggccaagc tggccggcca
     8701 ggccgccagc ttggcatcag acaaaccggc caggatttca tgcagccgca cggttgagac
     8761 gtgcgcgggc ggctcgaaca cgtacccggc cgcgatcatc tccgcctcga tctcttcggt
     8821 aatgaaaaac ggttcgtcct ggccgtcctg gtgcggtttc atgcttgttc ctcttggcgt
     8881 tcattctcgg cggccgccag ggcgtcggcc tcggtcaatg cgtcctcacg gaaggcaccg
     8941 cgccgcctgg cctcggtggg cgtcacttcc tcgctgcgct caagtgcgcg gtacagggtc
     9001 gagcgatgca cgccaagcag tgcagccgcc tctttcacgg tgcggccttc ctggtcgatc
     9061 agctcgcggg cgtgcgcgat ctgtgccggg gtgagggtag ggcgggggcc aaacttcacg
     9121 cctcgggcct tggcggcctc gcgcccgctc cgggtgcggt cgatgattag ggaacgctcg
     9181 aactcggcaa tgccggcgaa cacggtcaac accatgcggc cggccggcgt ggtggtgtcg
     9241 gcccacggct ctgccaggct acgcaggccc gcgccggcct cctggatgcg ctcggcaatg
     9301 tccagtaggt cgcgggtgct gcgggccagg cggtctagcc tggtcactgt cacaacgtcg
     9361 ccagggcgta ggtggtcaag catcctggcc agctccgggc ggtcgcgcct ggtgccggtg
     9421 atcttctcgg aaaacagctt ggtgcagccg gccgcgtgca gttcggcccg ttggttggtc
     9481 aagtcctggt cgtcggtgct gacgcgggca tagcccagca ggccagcggc ggcgctcttg
     9541 ttcatggcgt aatgtctccg gttctagtcg caagtattct actttatgcg actaaaacac
     9601 gcgacaagaa aacgccagga aaagggcagg gcggcagcct gtcgcgtaac ttaggacttg
     9661 tgcgacatgt cgttttcaga agacggctgc actgaacgtc agaagccgac tgcactatag
     9721 cagcggaggg gttggatcaa agtactttga tcccgagggg aaccctgtgg ttggcatgca
     9781 catacaaatg gacgaacgga taaacctttt cacgcccttt taaatatccg attattctaa
     9841 taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac tgatagttta
     9901 aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca ttcgccattc
     9961 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
    10021 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
    10081 cgacgttgta aaacgacggc cagtgccaag cttgcatgcc tgcaggtcct gctgagcctc
    10141 gacatgttgt cgcaaaattc gccctggacc cgcccaacga tttgtcgtca ctgtcaaggt
    10201 ttgacctgca cttcatttgg ggcccacata caccaaaaaa atgctgcata attctcgggg
    10261 cagcaagtcg gttacccggc cgccgtgctg gaccgggttg aatggtgccc gtaactttcg
    10321 gtagagcgga cggccaatac tcaacttcaa ggaatctcac ccatgcgcgc cggcggggaa
    10381 ccggagttcc cttcagtgag cgttattagt tcgccgctcg gtgtgtcgta gatactagcc
    10441 cctggggcac ttttgaaatt tgaataagat ttatgtaatc agtcttttag gtttgaccgg
    10501 ttctgccgct ttttttaaaa ttggatttgt aataataaaa cgcaattgtt tgttattgtg
    10561 gcgctctatc atagatgtcg ctataaacct attcagcaca atatattgtt ttcattttaa
    10621 tattgtacat ataagtagta gggtacaatc agtaaattga acggagaata ttattcataa
    10681 aaatacgata gtaacgggtg atatattcat tagaatgaac cgaaaccggc ggtaaggatc
    10741 tgagctacac atgctcaggt tttttacaac gtgcacaaca gaattgaaag caaatatcat
    10801 gcgatcatag gcgtctcgca tatctcatta aagcaggact ctagggacta gtcccgggtc
    10861 ttaattaagt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa ggagatcctt
    10921 tggatcacaa aaaaaaagcc cgctcattag gcgggttaaa ttcccgggta ctgcagccga
    10981 ttcattaatg caggctaacc tggcttatcg aaattaatac gactcactat agggagaccg
    11041 gcagatctga tccctacttc aaaaatgtca aagatacagt ctcagaagac caaagggcta
    11101 ttgagacttt tcaacaaagg gtaatttcgg gaaacctcct cggattccat tgcccagcta
    11161 tctgtcactt catcgaaagg acagtggaaa aggaaggtga ctcctacaaa tgccatcatt
    11221 gcgataaagg aaaggctatc attcaagatg cctctgccga cagtggtccc aaagatggac
    11281 ccccacccac gaggagcatc gtgggaaaag aagacgttcc aaccacgtct tcaaagcaag
    11341 tggattgatg tgacatctcc actgacgtaa gggatgacgc acaatcccac ccctactcca
    11401 aaaatgtcaa agataccgtc tcagaagacc aaagggctat tgggactttt caacaaaggg
    11461 taattt
//
