LOCUS       sequence-167208-        1009 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd Pro5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Tan CY, Hirakawa H, Suzuki R, Haga T, Iwata F, Nagamune T
  TITLE     Immobilization of a Bacterial Cytochrome P450 Monooxygenase System 
            on a Solid Support.
  JOURNAL   Angew Chem Int Ed Engl. 2016 Oct 26. doi: 10.1002/anie.201608033.
  PUBMED    27781345
REFERENCE   2  (bases 1 to 1009)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Angew Chem 
            Int Ed Engl. 2016 Oct 26. doi: 10.1002/anie.201608033."
COMMENT     Sequence Label: pHPro53
FEATURES             Location/Qualifiers
     source          1..1009
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    322..343
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(358..363,364..381,382..388)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    396..412
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     401..423
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     420..436
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     420..436
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(522..539)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(522..538)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(531..553)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(693..710)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(944..963)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgctgatgcc gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca
       61 gaatgcttaa tgaattacaa cagtactgcg atgagtggca gggcggggcg taattttttt
      121 aaggcagtta ttggtgccct taaacgcctg gttgctacgc ctgaataagt gataataagc
      181 ggatgaatgg cagaaattca gcttggccca gtgccaagct ccaatacgca aaccgcctct
      241 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
      301 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
      361 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
      421 aggaaacagc tatgaccatg attacgaatt ggtctcggcc accaccgccg cctccacgag
      481 accgatcctc tagagtcgac ctgcaggcat gcaagcttgg cactggccgt cgttttacaa
      541 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
      601 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
      661 agcctgaatg gcgaatgagc ttcttccgct tcctcgctca ctgactcgct gcgctcggtc
      721 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
      781 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
      841 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
      901 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
      961 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgctta
//
