LOCUS       sequence-167209-       11476 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  target  miRNA169d  for destruction in Arabidopsis thaliana.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11476)
  AUTHORS   Tang G, Yan J, Gu Y, Qiao M, Fan R, Mao Y, Tang X
  TITLE     Construction of short tandem target mimic (STTM) to block the 
            functions of plant and animal microRNAs.
  JOURNAL   Methods. 2012 Oct;58(2):118-25. doi: 10.1016/j.ymeth.2012.10.006. 
            Epub 2012 Oct 23.
  PUBMED    23098881
REFERENCE   2  (bases 1 to 11476)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ymeth.2012.10.006"; journalName: "Methods"; date: 
            "2012-10"; volume: "58"; issue: "2"; pages: "118-25"
COMMENT     Sequence Label: pFGC5941-PacI-ath-STTM169d
FEATURES             Location/Qualifiers
     source          1..11476
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..346
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     289..309
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     polyA_signal    507..683
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(1245..1263)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1246..1263)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             complement(1334..1993)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKEITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1908..1927
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(2030..2035,2036..2052,2053..2058))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     terminator      2320..3027
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     primer_bind     complement(3052..3069)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3052..3068)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3061..3083)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    3271..3295
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4596..5225
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5654..6727
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      6793..6987
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(7223..7245)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7331..7471
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7386..7405
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7486..7503)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7657..8245)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8332..9126)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    9551..9575
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(9661..9913)
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     CDS             complement(9923..10474)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(10480..10860)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     primer_bind     11051..11070
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        11051..11069
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tgagactttt caacaaaggg taatttcggg aaacctcctc ggattccatt gcccagctac
       61 ctgtcacctc atcgaaagga cagtagaaaa ggaaggtggc tcctacaaat gccatcatcg
      121 cgataaagga aaggctatca ttcaagatgc ctctgccgac agtggtccca aagatggacc
      181 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
      241 ggattgatgt gacatctcca ctgacataag ggatgacgca caaccccact atccttcgca
      301 agacccttcc tctatataag gaagttcatt tcatttggag aggacagccc aagcttcggc
      361 aagtcataaa ccttggctca gttgttgttg ttatggtcta atttaaatat ggtctaaaga
      421 agaagaatcg gcaagtcata aaccttggct cagaattcgg tacgctgaaa tcaccagtct
      481 ctctctacaa atctatctct ctctattttc tccataaata atgtgtgagt agtttcccga
      541 taagggaaat tagggttctt atagggtttc gctcatgtgt tgagcatata agaaaccctt
      601 agtatgtatt tgtatttgta aaatacttct atcaataaaa tttctaattc ctaaaaccaa
      661 aatccagtac taaaatccag atctcctaaa gtccctatag atctttgtcg tgaatataaa
      721 ccagacacga gacgactaga cctggagccc agacgccgtt cgaagctaga agtaccgctt
      781 aggcaggagg ccgttaggga aaagatgcta aggcagggtt ggttacgttg actcccccgt
      841 aggtttggtt taaatatgat gaagtggacg gaaggaagga ggaagacaag gaaggataag
      901 gttgcaggcc ctgtgcaagg taagaagatg gaaatttgat agaggtacgc tactatactt
      961 atactatacg ctaagggaat gattgtattt ataccctata ccccctaata accccttatc
     1021 aatttaagaa ataatccgca taagcccccg cttaaaaatt ggtatcagag ccatgaatag
     1081 gtctatgacc aaaactcaag aggataaaac ctcaccaaaa tacgaaagag tccttaactc
     1141 taaagataaa agatctttca agatcaaaac tagttccctc acaccggtga cggggatcgc
     1201 atgcgacctc tagaccatca cattggagct tcagctgctc gagttctata gtgtcaccta
     1261 aatcgtatgt gtatgataca taaggttatg tattaattgt agccgcgttc taacgtcgac
     1321 aggcctgtac caattacgcc ccgccctgcc actcatcgca gtactgttgt aattcattaa
     1381 gcattctgcc gacatggaag ccatcacaaa cggcatgatg aacctgaatc gccagcggca
     1441 tcagcacctt gtcgccttgc gtataatatt tgcccatggt gaaaacgggg gcgaagaagt
     1501 tgtccatatt ggccacgttt aaatcaaaac tggtgaaact cacccaggga ttggctgaga
     1561 cgaaaaacat attctcaata aaccctttag ggaaataggc caggttttca ccgtaacacg
     1621 ccacatcttg cgaatatatg tgtagaaact gccggaaatc gtcgtggtat tcactccaga
     1681 gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta acaagggtga acactatccc
     1741 atattaccag ctcaccgtct ttcattgcca tacggaactc cggatgagca ttcatcaggc
     1801 gggcaagaat gtgaataaag gccggataaa acttgtgctt atttttcttt acggtcttta
     1861 aaaaggccgt aatatccagc tgaacggtct ggttataggt acattgagca actgactgaa
     1921 atgcctcaaa atgttcttta cgatgccatt gggatatatc aacggtggta tatccagtga
     1981 tttctttctc cattttagct tccttagctc ctgaaaatct cgataactca ttaaagctta
     2041 tcgatgataa gctgtcaaac atgagaattt agcccgccta atgagcgggc tttttgctgt
     2101 gatccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     2161 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     2221 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     2281 gcctttctta attaagaccc gggactagtc cctagagtcc tgctttaatg agatatgcga
     2341 gacgcctatg atcgcatgat atttgctttc aattctgttg tgcacgttgt aaaaaacctg
     2401 agcatgtgta gctcagatcc ttaccgccgg tttcggttca ttctaatgaa tatatcaccc
     2461 gttactatcg tatttttatg aataatattc tccgttcaat ttactgattg taccctacta
     2521 cttatatgta caatattaaa atgaaaacaa tatattgtgc tgaataggtt tatagcgaca
     2581 tctatgatag agcgccacaa taacaaacaa ttgcgtttta ttattacaaa tccaatttta
     2641 aaaaaagcgg cagaaccggt caaacctaaa agactgatta cataaatctt attcaaattt
     2701 caaaagtgcc ccaggggcta gtatctacga cacaccgagc ggcgaactaa taacgctcac
     2761 tgaagggaac tccggttccc cgccggcgcg catgggtgag attccttgaa gttgagtatt
     2821 ggccgtccgc tctaccgaaa gttacgggca ccattcaacc cggtccagca cggcggccgg
     2881 gtaaccgact tgctgccccg agaattatgc agcatttttt tggtgtatgt gggccccaaa
     2941 tgaagtgcag gtcaaacctt gacagtgacg acaaatcgtt gggcgggtcc agggcgaatt
     3001 ttgcgacaac atgtcgaggc tcagcaggac ctgcaggcat gcaagcttgg cactggccgt
     3061 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     3121 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     3181 acagttgcgc agcctgaatg gcgaatgcta gagcagcttg agcttggatc agattgtcgt
     3241 ttcccgcctt cagtttaaac tatcagtgtt tgacaggata tattggcggg taaacctaag
     3301 agaaaagagc gtttattaga ataatcggat atttaaaagg gcgtgaaaag gtttatccgt
     3361 tcgtccattt gtatgtgcat gccaaccaca gggttcccct cgggatcaaa gtactttgat
     3421 ccaacccctc cgctgctata gtgcagtcgg cttctgacgt tcagtgcagc cgtcttctga
     3481 aaacgacatg tcgcacaagt cctaagttac gcgacaggct gccgccctgc ccttttcctg
     3541 gcgttttctt gtcgcgtgtt ttagtcgcat aaagtagaat acttgcgact agaaccggag
     3601 acattacgcc atgaacaaga gcgccgccgc tggcctgctg ggctatgccc gcgtcagcac
     3661 cgacgaccag gacttgacca accaacgggc cgaactgcac gcggccggct gcaccaagct
     3721 gttttccgag aagatcaccg gcaccaggcg cgaccgcccg gagctggcca ggatgcttga
     3781 ccacctacgc cctggcgacg ttgtgacagt gaccaggcta gaccgcctgg cccgcagcac
     3841 ccgcgaccta ctggacattg ccgagcgcat ccaggaggcc ggcgcgggcc tgcgtagcct
     3901 ggcagagccg tgggccgaca ccaccacgcc ggccggccgc atggtgttga ccgtgttcgc
     3961 cggcattgcc gagttcgagc gttccctaat catcgaccgc acccggagcg ggcgcgaggc
     4021 cgccaaggcc cgaggcgtga agtttggccc ccgccctacc ctcaccccgg cacagatcgc
     4081 gcacgcccgc gagctgatcg accaggaagg ccgcaccgtg aaagaggcgg ctgcactgct
     4141 tggcgtgcat cgctcgaccc tgtaccgcgc acttgagcgc agcgaggaag tgacgcccac
     4201 cgaggccagg cggcgcggtg ccttccgtga ggacgcattg accgaggccg acgccctggc
     4261 ggccgccgag aatgaacgcc aagaggaaca agcatgaaac cgcaccagga cggccaggac
     4321 gaaccgtttt tcattaccga agagatcgag gcggagatga tcgcggccgg gtacgtgttc
     4381 gagccgcccg cgcacgtctc aaccgtgcgg ctgcatgaaa tcctggccgg tttgtctgat
     4441 gccaagctgg cggcctggcc ggccagcttg gccgctgaag aaaccgagcg ccgccgtcta
     4501 aaaaggtgat gtgtatttga gtaaaacagc ttgcgtcatg cggtcgctgc gtatatgatg
     4561 cgatgagtaa ataaacaaat acgcaagggg aacgcatgaa ggttatcgct gtacttaacc
     4621 agaaaggcgg gtcaggcaag acgaccatcg caacccatct agcccgcgcc ctgcaactcg
     4681 ccggggccga tgttctgtta gtcgattccg atccccaggg cagtgcccgc gattgggcgg
     4741 ccgtgcggga agatcaaccg ctaaccgttg tcggcatcga ccgcccgacg attgaccgcg
     4801 acgtgaaggc catcggccgg cgcgacttcg tagtgatcga cggagcgccc caggcggcgg
     4861 acttggctgt gtccgcgatc aaggcagccg acttcgtgct gattccggtg cagccaagcc
     4921 cttacgacat atgggccacc gccgacctgg tggagctggt taagcagcgc attgaggtca
     4981 cggatggaag gctacaagcg gcctttgtcg tgtcgcgggc gatcaaaggc acgcgcatcg
     5041 gcggtgaggt tgccgaggcg ctggccgggt acgagctgcc cattcttgag tcccgtatca
     5101 cgcagcgcgt gagctaccca ggcactgccg ccgccggcac aaccgttctt gaatcagaac
     5161 ccgagggcga cgctgcccgc gaggtccagg cgctggccgc tgaaattaaa tcaaaactca
     5221 tttgagttaa tgaggtaaag agaaaatgag caaaagcaca aacacgctaa gtgccggccg
     5281 tccgagcgca cgcagcagca aggctgcaac gttggccagc ctggcagaca cgccagccat
     5341 gaagcgggtc aactttcagt tgccggcgga ggatcacacc aagctgaaga tgtacgcggt
     5401 acgccaaggc aagaccatta ccgagctgct atctgaatac atcgcgcagc taccagagta
     5461 aatgagcaaa tgaataaatg agtagatgaa ttttagcggc taaaggaggc ggcatggaaa
     5521 atcaagaaca accaggcacc gacgccgtgg aatgccccat gtgtggagga acgggcggtt
     5581 ggccaggcgt aagcggctgg gttgcctgcc ggccctgcaa tggcactgga acccccaagc
     5641 ccgaggaatc ggcgtgagcg gtcgcaaacc atccggcccg gtacaaatcg gcgcggcgct
     5701 gggtgatgac ctggtggaga agttgaaggc cgcgcaggcc gcccagcggc aacgcatcga
     5761 ggcagaagca cgccccggtg aatcgtggca agcggccgct gatcgaatcc gcaaagaatc
     5821 ccggcaaccg ccggcagccg gtgcgccgtc gattaggaag ccgcccaagg gcgacgagca
     5881 accagatttt ttcgttccga tgctctatga cgtgggcacc cgcgatagtc gcagcatcat
     5941 ggacgtggcc gttttccgtc tgtcgaagcg tgaccgacga gctggcgagg tgatccgcta
     6001 cgagcttcca gacgggcacg tagaggtttc cgcagggccg gccggcatgg ccagtgtgtg
     6061 ggattacgac ctggtactga tggcggtttc ccatctaacc gaatccatga accgataccg
     6121 ggaagggaag ggagacaagc ccggccgcgt gttccgtcca cacgttgcgg acgtactcaa
     6181 gttctgccgg cgagccgatg gcggaaagca gaaagacgac ctggtagaaa cctgcattcg
     6241 gttaaacacc acgcacgttg ccatgcagcg tacgaagaag gccaagaacg gccgcctggt
     6301 gacggtatcc gagggtgaag ccttgattag ccgctacaag atcgtaaaga gcgaaaccgg
     6361 gcggccggag tacatcgaga tcgagctagc tgattggatg taccgcgaga tcacagaagg
     6421 caagaacccg gacgtgctga cggttcaccc cgattacttt ttgatcgatc ccggcatcgg
     6481 ccgttttctc taccgcctgg cacgccgcgc cgcaggcaag gcagaagcca gatggttgtt
     6541 caagacgatc tacgaacgca gtggcagcgc cggagagttc aagaagttct gtttcaccgt
     6601 gcgcaagctg atcgggtcaa atgacctgcc ggagtacgat ttgaaggagg aggcggggca
     6661 ggctggcccg atcctagtca tgcgctaccg caacctgatc gagggcgaag catccgccgg
     6721 ttcctaatgt acggagcaga tgctagggca aattgcccta gcaggggaaa aaggtcgaaa
     6781 aggtctcttt cctgtggata gcacgtacat tgggaaccca aagccgtaca ttgggaaccg
     6841 gaacccgtac attgggaacc caaagccgta cattgggaac cggtcacaca tgtaagtgac
     6901 tgatataaaa gagaaaaaag gcgatttttc cgcctaaaac tctttaaaac ttattaaaac
     6961 tcttaaaacc cgcctggcct gtgcataact gtctggccag cgcacagccg aagagctgca
     7021 aaaagcgcct acccttcggt cgctgcgctc cctacgcccc gccgcttcgc gtcggcctat
     7081 cgcggccgct ggccgctcaa aaatggctgg cctacggcca ggcaatctac cagggcgcgg
     7141 acaagccgcg ccgtcgccac tcgaccgccg gcgcccacat caaggcaccc tgcctcgcgc
     7201 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
     7261 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
     7321 ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat actggcttaa
     7381 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca
     7441 cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc
     7501 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     7561 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     7621 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     7681 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     7741 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     7801 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     7861 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     7921 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     7981 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     8041 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
     8101 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     8161 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     8221 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     8281 tcagtggaac gaaaactcac gttaagggat tttggtcatg cattctaggt actaaaacaa
     8341 ttcatccagt aaaatataat attttatttt ctcccaatca ggcttgatcc ccagtaagtc
     8401 aaaaaatagc tcgacatact gttcttcccc gatatcctcc ctgatcgacc ggacgcagaa
     8461 ggcaatgtca taccacttgt ccgccctgcc gcttctccca agatcaataa agccacttac
     8521 tttgccatct ttcacaaaga tgttgctgtc tcccaggtcg ccgtgggaaa agacaagttc
     8581 ctcttcgggc ttttccgtct ttaaaaaatc atacagctcg cgcggatctt taaatggagt
     8641 gtcttcttcc cagttttcgc aatccacatc ggccagatcg ttattcagta agtaatccaa
     8701 ttcggctaag cggctgtcta agctattcgt atagggacaa tccgatatgt cgatggagtg
     8761 aaagagcctg atgcactccg catacagctc gataatcttt tcagggcttt gttcatcttc
     8821 atactcttcc gagcaaagga cgccatcggc ctcactcatg agcagattgc tccagccatc
     8881 atgccgttca aagtgcagga cctttggaac aggcagcttt ccttccagcc atagcatcat
     8941 gtccttttcc cgttccacat cataggtggt ccctttatac cggctgtccg tcatttttaa
     9001 atataggttt tcattttctc ccaccagctt atatacctta gcaggagaca ttccttccgt
     9061 atcttttacg cagcggtatt tttcgatcag ttttttcaat tccggtgata ttctcatttt
     9121 agccatttat tatttccttc ctcttttcta cagtatttaa agatacccca agaagctaat
     9181 tataacaaga cgaactccaa ttcactgttc cttgcattct aaaaccttaa ataccagaaa
     9241 acagcttttt caaagttgtt ttcaaagttg gcgtataaca tagtatcgac ggagccgatt
     9301 ttgaaaccgc ggtgatcaca ggcagcaacg ctctgtcatc gttacaatca acatgctacc
     9361 ctccgcgaga tcatccgtgt ttcaaacccg gcagcttagt tgccgttctt ccgaatagca
     9421 tcggtaacat gagcaaagtc tgccgcctta caacggctct cccgctgacg ccgtcccgga
     9481 ctgatgggct gcctgtatcg agtggtgatt ttgtgccgag ctgccggtcg gggagctgtt
     9541 ggctggctgg tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata
     9601 acacattgcg gacgttttta atgtactgaa ttaacgccga attaattcga gctcggatct
     9661 gataatttat ttgaaaattc ataagaaaag caaacgttac atgaattgat gaaacaatac
     9721 aaagacagat aaagccacgc acatttagga tattggccga gattactgaa tattgagtaa
     9781 gatcacggaa tttctgacag gagcatgtct tcaattcagc ccaaatggca gttgaaatac
     9841 tcaaaccgcc ccatatgcag gagcggatca ttcattgttt gtttggttgc ctttgccaac
     9901 atgggagtcc aagattctgc agtcaaatct cggtgacggg caggaccgga cggggcggta
     9961 ccggcaggct gaagtccagc tgccagaaac ccacgtcatg ccagttcccg tgcttgaagc
    10021 cggccgcccg cagcatgccg cggggggcat atccgagcgc ctcgtgcatg cgcacgctcg
    10081 ggtcgttggg cagcccgatg acagcgacca cgctcttgaa gccctgtgcc tccagggact
    10141 tcagcaggtg ggtgtagagc gtggagccca gtcccgtccg ctggtggcgg ggggagacgt
    10201 acacggtcga ctcggccgtc cagtcgtagg cgttgcgtgc cttccagggg cccgcgtagg
    10261 cgatgccggc gacctcgccg tccacctcgg cgacgagcca gggatagcgc tcccgcagac
    10321 ggacgaggtc gtccgtccac tcctgcggtt cctgcggctc ggtacggaag ttgaccgtgc
    10381 ttgtctcgat gtagtggttg acgatggtgc agaccgccgg catgtccgcc tcggtggcac
    10441 ggcggatgtc ggccgggcgt cgttctgggc tcatcgattc gatttggtgt atcgagattg
    10501 gttatgaaat tcagatgcta gtgtaatgta ttggtaattt gggaagatat aataggaagc
    10561 aaggctattt atccatttct gaaaaggcga aatggcgtca ccgcgagcgt cacgcgcatt
    10621 ccgttcttgc tgtaaagcgt tgtttggtac acttttgact agcgaggctt ggcgtgtcag
    10681 cgtatctatt caaaagtcgt taatggctgc ggatcaagaa aaagttggaa tagaaacaga
    10741 atacccgcga aattcaggcc cggttgccat gtcctacacg ccgaaataaa cgaccaaatt
    10801 agtagaaaaa taaaaactga ctcggatact tacgtcacgt cttgcgcact gatttgaaaa
    10861 atctcagaat tcatggatcc taggtgagtc tagagagtta attaagtttg caagcagcag
    10921 attacgcgca gaaaaaaagg atctcaagga gatcctttgg atcacaaaaa aaagcccgct
    10981 cattaggcgg gttaaattcc cgggtactgc agccgattca ttaatgcagg ttaacctggc
    11041 ttatcgaaat taatacgact cactataggg agaccggtag atctgatccc tactccaaaa
    11101 atgtcaaaga tacagtctca gaagaccaaa gggctattga gacttttcaa caaagggtaa
    11161 tttcgggaaa cctcctcgga ttccattgcc cagctatctg tcacttcatc gaaaggacag
    11221 tggaaaagga aggtggctcc tacaaatgcc atcattgcga taaaggaaag gctatcattc
    11281 aagatgcctc tgccgacagt ggtcccaaag atggaccccc acccacgagg agcatcgtgg
    11341 gaaaagaaga cgttccaacc acgtcttcaa agcaagtgga ttgatgtgac atctccactg
    11401 acgtaaggga tgacgcacaa tcccacccct actccaaaaa tgtcaaagat actgtctcag
    11461 aagaccaaag ggctat
//
