LOCUS       sequence-16721-e        1409 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1997
REFERENCE   2  (bases 1 to 1409)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: L3620
FEATURES             Location/Qualifiers
     source          1..1409
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             341..361
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(404..432)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 agaggncccc aattcttgag acttccacta tcttctctgg caccctccac tacgcgtagg
       61 cgtctctcgc ttcgtgtatt cccgggaagc cgggttcccg tctctcccgc cgctgccgct
      121 gccgcacaca gctttacacc tcgtagaatc cccaaagagg ggcgtggctt gcgggtgcca
      181 acatcctcct gccgaggaag aagcaggcac tcatcactcg catcatcaac ctcgggattg
      241 gccaaaggac ccaaaggtat gtttcgaatg atactaacat aacatagaac attttcagga
      301 ggacccttgg agggtaccga gctcagaaaa aatgactgct ccaaagaaga agcgtaaggt
      361 accggtagaa aaaatgagta aaggagaaga acttttcact ggagttgtcc caattcttgt
      421 tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga
      481 tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc
      541 atgggtaagt ttaaacatat atatactaac taaccctgat tatttaaatt ttcagccaac
      601 acttgtcact actctcactt atggtgttca atgcttctcg agatacccag atcatatgaa
      661 acggcatgac tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa gaactatatt
      721 tttcaaagat gacgggaact acaagacacg taagtttaaa cagttcggta ctaactaacc
      781 atacatattt aaattttcag gtgctgaagt caagtttgaa ggtgataccc ttgttaatag
      841 aatcgagtta aaaggtattt gattttaaag aagatggaaa catttcttgg acacaaattg
      901 gaatacaact ataactcaca ccatgtatan cttcgcagca gacaacaaaa gaatggaaat
      961 caaggttgta aagtttaaac atgattttac taactaacta atcctgattt aaattttcng
     1021 aacttcaaaa ttnagaccca aatttgaaga anggaagcgt ttcaactagc canaacnttt
     1081 tcaacaaaat actccattgg gngaaggccc tntcttttac canaaaaccn tnactgtccc
     1141 naatccnncc nttcnaaaaa tccannaaaa naaaaacnat ggccttctgn nttntancct
     1201 ncggaanacc cngncnggan aactnanaag cccgggggga aannaaaaac ccccaatgac
     1261 cganttccnc gtttnccngt tggggaanan nccntttaan gcgnnggnaa cggggncaat
     1321 accctgaacc cttncccggn aaaaaccccc cccnnccngn nnttannnna atttttantt
     1381 ttgnngtttt tccccnncga ggttnttna
//
