LOCUS       sequence-167216-       11471 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  target  miRNA172  for destruction in Arabidopsis thaliana.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11471)
  AUTHORS   Tang G, Yan J, Gu Y, Qiao M, Fan R, Mao Y, Tang X
  TITLE     Construction of short tandem target mimic (STTM) to block the 
            functions of plant and animal microRNAs.
  JOURNAL   Methods. 2012 Oct;58(2):118-25. doi: 10.1016/j.ymeth.2012.10.006. 
            Epub 2012 Oct 23.
  PUBMED    23098881
REFERENCE   2  (bases 1 to 11471)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ymeth.2012.10.006"; journalName: "Methods"; date: 
            "2012-10"; volume: "58"; issue: "2"; pages: "118-25"
COMMENT     Sequence Label: pFGC5941-PacI-ath-STTM172
FEATURES             Location/Qualifiers
     source          1..11471
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        126..471
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     414..434
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     polyA_signal    632..808
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(1370..1388)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1371..1388)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     2027..2046
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(2149..2154,2155..2171,2172..2177))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     terminator      2439..3146
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     primer_bind     complement(3171..3188)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3171..3187)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3180..3202)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    3390..3414
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4715..5344
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5773..6846
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      6912..7106
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(7342..7364)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7450..7590
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7505..7524
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7605..7622)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7776..8364)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8451..9245)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    9670..9694
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(9780..10032)
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     CDS             complement(10042..10593)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(10599..10979)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     primer_bind     11171..11190
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        11171..11189
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gttccaacca cgtcttcaaa gcaagtggat tgatgtgaca tctccactga cgtaagggat
       61 gacgcacaat cccaccccta ctccaaaaat gtcaaagata ctgtctcaga agaccaaagg
      121 gctattgaga cttttcaaca aagggtaatt tcgggaaacc tcctcggatt ccattgccca
      181 gctatctgtc acctcatcga aaggacagta gaaaaggaag gtggctccta caaatgccat
      241 cattgcgata aaggaaaggc tatcattcaa gatgcctctg ccgacagtgg tcccaaagat
      301 ggacccccac ccacgaggag catcgtggaa aaagaagacg ttccaaccac gtcttcaaag
      361 caagtggatt gatgtgacat ctccactgac ataagggatg acgcacaacc ccactatcct
      421 tcgcaagacc cttcctctat ataaggaagt tcatttcatt tggagaggac agcccaagct
      481 tatgcagcat cactatcaag attctgttgt tgttgttatg gtctaattta aatatggtct
      541 aaagaagaag aatatgcagc atcactatca agattcagaa ttcggtacgc tgaaatcacc
      601 agtctctctc tacaaatcta tctctctcta ttttctccat aaataatgtg tgagtagttt
      661 cccgataagg gaaattaggg ttcttatagg gtttcgctca tgtgttgagc atataagaaa
      721 cccttagtat gtatttgtat ttgtaaaata cttctatcaa taaaatttct aattcctaaa
      781 accaaaatcc ggtactaaaa tccagatctc ctaaagtccc tatagatctt tgtcgtgaat
      841 ataaaccaga cacgagacga ctagacctgg agcccagacg ccgttcgaag ctagaagtac
      901 cgcttaggca ggaggccgtt agggaaaaga tgctaaggca gggttggtta cgttgactcc
      961 cccgtaggtt tggtttaaat atgatgaagt ggacggaagg aaggaggaag acaaggaagg
     1021 ataaggttgc aggccctgtg caaggtaaga agatggaaat ttgatagagg tacgctacta
     1081 tacttatact atacgctaag ggaatgattg tatttatacc ctataccccc taataacccc
     1141 ttatcaattt aagaaataat ccgcataagc ccccgcttaa aaattggtat cagagccatg
     1201 aataggtcta tgaccaaaac tcaagaggat aaaacctcac caaaatacga aagagtcctt
     1261 aactctaaag ataaaagatc tttcaagatc aaaactagtt ccctcacacc ggtgacgggg
     1321 atcgcatgcg acctctagac catcacattg gagcttcagc tgctcgagtt ctatagtgtc
     1381 acctaaatcg tatgtgtatg atacataagg ttatgtatta attgtagccg cgttcttacg
     1441 tcgacgtacc aattacgccc cgccctgtca ctcatcgcag tactgttgta attcattaag
     1501 cattctgccg acatggaagc catcacaaac ggcatgatga acctgaatcg ccagcggcat
     1561 cagcaccttg tcgccttgcg tataatattt gcccatggtg aaaacggggg cgaagaagtt
     1621 gtccatattg gccacgttta aatcaaaact ggtgaaactc acccagggat tggctgagac
     1681 gaaaaacata ttctcaataa accctttagg gaaataggcc aggttttcac cgtaacacgc
     1741 cacatcttgc gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt cactccagag
     1801 cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa caagggtgaa cactatccca
     1861 tattaccagc tcaccgtctt tcattgccat acggaactcc ggatgagcat tcatcaggcg
     1921 ggcaagaatg tgaataaagg ccggataaaa cttgtgctta tttttcttta cggtctttaa
     1981 aaaggccgta atatccagct gaacggtctg gttataggta cattgagcaa ctgactgaaa
     2041 tgcctcaaaa tgttctttac gatgccattg ggatatatca acggtggtat atccagtgat
     2101 ttctttctcc attttagctt ccttagctcc tgaaaatctc gataactcat taaagcttat
     2161 cgatgataag ctgtcaaaca tgagaattta gcccgcctaa tgagcgggct ttttgttgtg
     2221 atccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     2281 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     2341 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     2401 cctttcttaa ttaagacccg ggactagtcc ctagagtcct gctttaatga gatatgcgag
     2461 acgcctatga tcgcatgata tttgctttca attctgttgt gcacgttgta aaaaacctga
     2521 gcatgtgtag ctcagatcct taccgccggt ttcggttcat tctaatgaat atatcacccg
     2581 ttactatcgt atttttatga ataatattct ccgttcaatt tactgattgt accctactac
     2641 ttatatgtac aatattaaaa tgaaaacaat atattgtgct gaataggttt atagcgacat
     2701 ctatgataga gcgccacaat aacaaacaat tgcgttttat tattacaaat ccaattttaa
     2761 aaaaagcggc agaaccggtc aaacctaaaa gactgattac ataaatctta ttcaaatttc
     2821 aaaagtgccc caggggctag tatctacgac acaccgagcg gcgaactaat aacgctcact
     2881 gaagggaact ccggttcccc gccggcgcgc atgggtgaga ttccttgaag ttgagtattg
     2941 gccgtccgct ctaccgaaag ttacgggcac cattcaaccc ggtccagcac ggcggccggg
     3001 taaccgactt gctgccccga gaattatgca gcattttttt ggtgtatgtg ggccccaaat
     3061 gaagtgcagg tcaaaccttg acagtgacga caaatcgttg ggcgggtcca gggcgaattt
     3121 tgcgacaaca tgtcgaggct cagcaggacc tgcaggcatg caagcttggc actggccgtc
     3181 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     3241 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     3301 cagttgcgca gcctgaatgg cgaatgctag agcagcttga gcttggatca gattgtcgtt
     3361 tcccgccttc agtttaaact atcagtgttt gacaggatat attggcgggt aaacctaaga
     3421 gaaaagagcg tttattagaa taatcggata tttaaaaggg cgtgaaaagg tttatccgtt
     3481 cgtccatttg tatgtgcatg ccaaccacag ggttcccctc gggatcaaag tactttgatc
     3541 caacccctcc gctgctatag tgcagtcggc ttctgacgtt cagtgcagcc gtcttctgaa
     3601 aacgacatgt cgcacaagtc ctaagttacg cgacaggctg ccgccctgcc cttttcctgg
     3661 cgttttcttg tcgcgtgttt tagtcgcata aagtagaata cttgcgacta gaaccggaga
     3721 cattacgcca tgaacaagag cgccgccgct ggcctgctgg gctatgcccg cgtcagcacc
     3781 gacgaccagg acttgaccaa ccaacgggcc gaactgcacg cggccggctg caccaagctg
     3841 ttttccgaga agatcaccgg caccaggcgc gaccgcccgg agctggccag gatgcttgac
     3901 cacctacgcc ctggcgacgt tgtgacagtg accaggctag accgcctggc ccgcagcacc
     3961 cgcgacctac tggacattgc cgagcgcatc caggaggccg gcgcgggcct gcgtagcctg
     4021 gcagagccgt gggccgacac caccacgccg gccggccgca tggtgttgac cgtgttcgcc
     4081 ggcattgccg agttcgagcg ttccctaatc atcgaccgca cccggagcgg gcgcgaggcc
     4141 gccaaggccc gaggcgtgaa gtttggcccc cgccctaccc tcaccccggc acagatcgcg
     4201 cacgcccgcg agctgatcga ccaggaaggc cgcaccgtga aagaggcggc tgcactgctt
     4261 ggcgtgcatc gctcgaccct gtaccgcgca cttgagcgca gcgaggaagt gacgcccacc
     4321 gaggccaggc ggcgcggtgc cttccgtgag gacgcattga ccgaggccga cgccctggcg
     4381 gccgccgaga atgaacgcca agaggaacaa gcatgaaacc gcaccaggac ggccaggacg
     4441 aaccgttttt cattaccgaa gagatcgagg cggagatgat cgcggccggg tacgtgttcg
     4501 agccgcccgc gcacgtctca accgtgcggc tgcatgaaat cctggccggt ttgtctgatg
     4561 ccaagctggc ggcctggccg gccagcttgg ccgctgaaga aaccgagcgc cgccgtctaa
     4621 aaaggtgatg tgtatttgag taaaacagct tgcgtcatgc ggtcgctgcg tatatgatgc
     4681 gatgagtaaa taaacaaata cgcaagggga acgcatgaag gttatcgctg tacttaacca
     4741 gaaaggcggg tcaggcaaga cgaccatcgc aacccatcta gcccgcgccc tgcaactcgc
     4801 cggggccgat gttctgttag tcgattccga tccccagggc agtgcccgcg attgggcggc
     4861 cgtgcgggaa gatcaaccgc taaccgttgt cggcatcgac cgcccgacga ttgaccgcga
     4921 cgtgaaggcc atcggccggc gcgacttcgt agtgatcgac ggagcgcccc aggcggcgga
     4981 cttggctgtg tccgcgatca aggcagccga cttcgtgctg attccggtgc agccaagccc
     5041 ttacgacata tgggccaccg ccgacctggt ggagctggtt aagcagcgca ttgaggtcac
     5101 ggatggaagg ctacaagcgg cctttgtcgt gtcgcgggcg atcaaaggca cgcgcatcgg
     5161 cggtgaggtt gccgaggcgc tggccgggta cgagctgccc attcttgagt cccgtatcac
     5221 gcagcgcgtg agctacccag gcactgccgc cgccggcaca accgttcttg aatcagaacc
     5281 cgagggcgac gctgcccgcg aggtccaggc gctggccgct gaaattaaat caaaactcat
     5341 ttgagttaat gaggtaaaga gaaaatgagc aaaagcacaa acacgctaag tgccggccgt
     5401 ccgagcgcac gcagcagcaa ggctgcaacg ttggccagcc tggcagacac gccagccatg
     5461 aagcgggtca actttcagtt gccggcggag gatcacacca agctgaagat gtacgcggta
     5521 cgccaaggca agaccattac cgagctgcta tctgaataca tcgcgcagct accagagtaa
     5581 atgagcaaat gaataaatga gtagatgaat tttagcggct aaaggaggcg gcatggaaaa
     5641 tcaagaacaa ccaggcaccg acgccgtgga atgccccatg tgtggaggaa cgggcggttg
     5701 gccaggcgta agcggctggg ttgcctgccg gccctgcaat ggcactggaa cccccaagcc
     5761 cgaggaatcg gcgtgagcgg tcgcaaacca tccggcccgg tacaaatcgg cgcggcgctg
     5821 ggtgatgacc tggtggagaa gttgaaggcc gcgcaggccg cccagcggca acgcatcgag
     5881 gcagaagcac gccccggtga atcgtggcaa gcggccgctg atcgaatccg caaagaatcc
     5941 cggcaaccgc cggcagccgg tgcgccgtcg attaggaagc cgcccaaggg cgacgagcaa
     6001 ccagattttt tcgttccgat gctctatgac gtgggcaccc gcgatagtcg cagcatcatg
     6061 gacgtggccg ttttccgtct gtcgaagcgt gaccgacgag ctggcgaggt gatccgctac
     6121 gagcttccag acgggcacgt agaggtttcc gcagggccgg ccggcatggc cagtgtgtgg
     6181 gattacgacc tggtactgat ggcggtttcc catctaaccg aatccatgaa ccgataccgg
     6241 gaagggaagg gagacaagcc cggccgcgtg ttccgtccac acgttgcgga cgtactcaag
     6301 ttctgccggc gagccgatgg cggaaagcag aaagacgacc tggtagaaac ctgcattcgg
     6361 ttaaacacca cgcacgttgc catgcagcgt acgaagaagg ccaagaacgg ccgcctggtg
     6421 acggtatccg agggtgaagc cttgattagc cgctacaaga tcgtaaagag cgaaaccggg
     6481 cggccggagt acatcgagat cgagctagct gattggatgt accgcgagat cacagaaggc
     6541 aagaacccgg acgtgctgac ggttcacccc gattactttt tgatcgatcc cggcatcggc
     6601 cgttttctct accgcctggc acgccgcgcc gcaggcaagg cagaagccag atggttgttc
     6661 aagacgatct acgaacgcag tggcagcgcc ggagagttca agaagttctg tttcaccgtg
     6721 cgcaagctga tcgggtcaaa tgacctgccg gagtacgatt tgaaggagga ggcggggcag
     6781 gctggcccga tcctagtcat gcgctaccgc aacctgatcg agggcgaagc atccgccggt
     6841 tcctaatgta cggagcagat gctagggcaa attgccctag caggggaaaa aggtcgaaaa
     6901 ggtctctttc ctgtggatag cacgtacatt gggaacccaa agccgtacat tgggaaccgg
     6961 aacccgtaca ttgggaaccc aaagccgtac attgggaacc ggtcacacat gtaagtgact
     7021 gatataaaag agaaaaaagg cgatttttcc gcctaaaact ctttaaaact tattaaaact
     7081 cttaaaaccc gcctggcctg tgcataactg tctggccagc gcacagccga agagctgcaa
     7141 aaagcgccta cccttcggtc gctgcgctcc ctacgccccg ccgcttcgcg tcggcctatc
     7201 gcggccgctg gccgctcaaa aatggctggc ctacggccag gcaatctacc agggcgcgga
     7261 caagccgcgc cgtcgccact cgaccgccgg cgcccacatc aaggcaccct gcctcgcgcg
     7321 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
     7381 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg
     7441 gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttaac
     7501 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac
     7561 agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg
     7621 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     7681 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     7741 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     7801 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     7861 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     7921 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     7981 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     8041 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     8101 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     8161 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
     8221 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     8281 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     8341 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     8401 cagtggaacg aaaactcacg ttaagggatt ttggtcatgc attctaggta ctaaaacaat
     8461 tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc cagtaagtca
     8521 aaaaatagct cgacatactg ttcttccccg atatcctccc tgatcgaccg gacgcagaag
     8581 gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa gccacttact
     8641 ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc
     8701 tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt aaatggagtg
     8761 tcttcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa gtaatccaat
     8821 tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc gatggagtga
     8881 aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg ttcatcttca
     8941 tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct ccagccatca
     9001 tgccgttcaa agtgcaggac ctttggaaca ggcagctttc cttccagcca tagcatcatg
     9061 tccttttccc gttccacatc ataggtggtc cctttatacc ggctgtccgt catttttaaa
     9121 tataggtttt cattttctcc caccagctta tataccttag caggagacat tccttccgta
     9181 tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat tctcatttta
     9241 gccatttatt atttccttcc tcttttctac agtatttaaa gataccccaa gaagctaatt
     9301 ataacaagac gaactccaat tcactgttcc ttgcattcta aaaccttaaa taccagaaaa
     9361 cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg gagccgattt
     9421 tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc
     9481 tccgcgagat catccgtgtt tcaaacccgg cagcttagtt gccgttcttc cgaatagcat
     9541 cggtaacatg agcaaagtct gccgccttac aacggctctc ccgctgacgc cgtcccggac
     9601 tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg
     9661 gctggctggt ggcaggatat attgtggtgt aaacaaattg acgcttagac aacttaataa
     9721 cacattgcgg acgtttttaa tgtactgaat taacgccgaa ttaattcgag ctcggatctg
     9781 ataatttatt tgaaaattca taagaaaagc aaacgttaca tgaattgatg aaacaataca
     9841 aagacagata aagccacgca catttaggat attggccgag attactgaat attgagtaag
     9901 atcacggaat ttctgacagg agcatgtctt caattcagcc caaatggcag ttgaaatact
     9961 caaaccgccc catatgcagg agcggatcat tcattgtttg tttggttgcc tttgccaaca
    10021 tgggagtcca agattctgca gtcaaatctc ggtgacgggc aggaccggac ggggcggtac
    10081 cggcaggctg aagtccagct gccagaaacc cacgtcatgc cagttcccgt gcttgaagcc
    10141 ggccgcccgc agcatgccgc ggggggcata tccgagcgcc tcgtgcatgc gcacgctcgg
    10201 gtcgttgggc agcccgatga cagcgaccac gctcttgaag ccctgtgcct ccagggactt
    10261 cagcaggtgg gtgtagagcg tggagcccag tcccgtccgc tggtggcggg gggagacgta
    10321 cacggtcgac tcggccgtcc agtcgtaggc gttgcgtgcc ttccaggggc ccgcgtaggc
    10381 gatgccggcg acctcgccgt ccacctcggc gacgagccag ggatagcgct cccgcagacg
    10441 gacgaggtcg tccgtccact cctgcggttc ctgcggctcg gtacggaagt tgaccgtgct
    10501 tgtctcgatg tagtggttga cgatggtgca gaccgccggc atgtccgcct cggtggcacg
    10561 gcggatgtcg gccgggcgtc gttctgggct catcgattcg atttggtgta tcgagattgg
    10621 ttatgaaatt cagatgctag tgtaatgtat tggtaatttg ggaagatata ataggaagca
    10681 aggctattta tccatttctg aaaaggcgaa atggcgtcac cgcgagcgtc acgcgcattc
    10741 cgttcttgct gtaaagcgtt gtttggtaca cttttgacta gcgaggcttg gcgtgtcagc
    10801 gtatctattc aaaagtcgtt aatggctgcg gatcaagaaa aagttggaat agaaacagaa
    10861 tacccgcgaa attcaggccc ggttgccatg tcctacacgc cgaaataaac gaccaaatta
    10921 gtagaaaaat aaaaactgac tcggatactt acgtcacgtc ttgcgcactg atttgaaaaa
    10981 tctcagaatt catggatcct aggtgagtct agagagttaa ttaagtttgc aagcagcaga
    11041 ttacgcgcag aaaaaaagga tctcaaggag atcctttgga tcacaaaaaa aaagcccgct
    11101 cattaggcgg gttaaattcc cgggtactgc agccgattca ttaatgcagg ttaacctggc
    11161 ttatcgaaat taatacgact cactataggg agaccggcag acctgatccc tactccaaaa
    11221 atgtcaaaga tacagtctca gaagaccaaa gggctattga gacttttcaa caaagggtaa
    11281 tttcgggaaa cctcctcgga ttccattgcc cagctatctg tcacttcatc gaaaggacag
    11341 tggaaaagga aggtggctcc tacaaatgcc atcattgcga taaaggaaag gctatcattc
    11401 aagatgcctc tgccgacagt ggtcccaaag atggaccccc acccacgagg agcatcgtgg
    11461 gaaaagaaga c
//
