LOCUS       sequence-167219-       10423 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  target  miRNA1886.2  for destruction in Arabidopsis thaliana.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10423)
  AUTHORS   Tang G, Yan J, Gu Y, Qiao M, Fan R, Mao Y, Tang X
  TITLE     Construction of short tandem target mimic (STTM) to block the 
            functions of plant and animal microRNAs.
  JOURNAL   Methods. 2012 Oct;58(2):118-25. doi: 10.1016/j.ymeth.2012.10.006. 
            Epub 2012 Oct 23.
  PUBMED    23098881
REFERENCE   2  (bases 1 to 10423)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ymeth.2012.10.006"; journalName: "Methods"; date: 
            "2012-10"; volume: "58"; issue: "2"; pages: "118-25"
COMMENT     Sequence Label: pFGC5941-PacI-ath-STTM1886.2
FEATURES             Location/Qualifiers
     source          1..10423
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             129..1202
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      1268..1462
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(1698..1720)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    1806..1946
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     1861..1880
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(1961..1978)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2132..2720)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2807..3601)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    4026..4050
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(4136..4388)
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     CDS             complement(4398..4949)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(4955..5335)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     promoter        join(5610..5615,5616..5632,5633..5638)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     promoter        complement(6149..6167)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6150..6167)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        complement(join(6928..6933,6934..6950,6951..6956))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     terminator      7218..7925
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     primer_bind     complement(7950..7967)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7950..7966)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7959..7981)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    8169..8193
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             9494..10123
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 gaacaaccag gcaccgacgc cgtggaatgc cccatgtgtg gaggaacggg cggttggcca
       61 ggcgtaagcg gctgggttgc ctgccggccc tgcaatggca ctggaacccc caagcccgag
      121 gaatcggcgt gagcggtcgc aaaccatccg gcccggtaca aatcggcgcg gcgctgggtg
      181 atgacctggt ggagaagttg aaggccgcgc aggccgccca gcggcaacgc atcgaggcag
      241 aagcacgccc cggtgaatcg tggcaagcgg ccgctgatcg aatccgcaaa gaatcccggc
      301 aaccgccggc agccggtgcg ccgtcgatta ggaagccgcc caagggcgac gagcaaccag
      361 attttttcgt tccgatgctc tatgacgtgg gcacccgcga tagtcgcagc atcatggacg
      421 tggccgtttt ccgtctgtcg aagcgtgacc gacgagctgg cgaggtgatc cgctacgagc
      481 ttccagacgg gcacgtagag gtttccgcag ggccggccgg catggccagt gtgtgggatt
      541 acgacctggt actgatggcg gtttcccatc taaccgaatc catgaaccga taccgggaag
      601 ggaagggaga caagcccggc cgcgtgttcc gtccacacgt tgcggacgta ctcaagttct
      661 gccggcgagc cgatggcgga aagcagaaag acgacctggt agaaacctgc attcggttaa
      721 acaccacgca cgttgccatg cagcgtacga agaaggccaa gaacggccgc ctggtgacgg
      781 tatccgaggg tgaagccttg attagccgct acaagatcgt aaagagcgaa accgggcggc
      841 cggagtacat cgagatcgag ctagctgatt ggatgtaccg cgagatcaca gaaggcaaga
      901 acccggacgt gctgacggtt caccccgatt actttttgat cgatcccggc atcggccgtt
      961 ttctctaccg cctggcacgc cgcgccgcag gcaaggcaga agccagatgg ttgttcaaga
     1021 cgatctacga acgcagtggc agcgccggag agttcaagaa gttctgtttc accgtgcgca
     1081 agctgatcgg gtcaaatgac ctgccggagt acgatttgaa ggaggaggcg gggcaggctg
     1141 gcccgatcct agtcatgcgc taccgcaacc tgatcgaggg cgaagcatcc gccggttcct
     1201 aatgtacgga gcagatgcta gggcaaattg ccctagcagg ggaaaaaggt cgaaaaggtc
     1261 tctttcctgt ggatagcacg tacattggga acccaaagcc gtacattggg aaccggaacc
     1321 cgtacattgg gaacccaaag ccgtacattg ggaaccggtc acacatgtaa gtgactgata
     1381 taaaagagaa aaaaggcgat ttttccgcct aaaactcttt aaaacttatt aaaactctta
     1441 aaacccgcct ggcctgtgca taactgtctg gccagcgcac agccgaagag ctgcaaaaag
     1501 cgcctaccct tcggtcgctg cgctccctac gccccgccgc ttcgcgtcgg cctatcgcgg
     1561 ccgctggccg ctcaaaaatg gctggcctac ggccaggcaa tctaccaggg cgcggacaag
     1621 ccgcgccgtc gccactcgac cgccggcgcc cacatcaagg caccctgcct cgcgcgtttc
     1681 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg
     1741 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
     1801 cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg
     1861 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat
     1921 gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc
     1981 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     2041 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     2101 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     2161 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     2221 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     2281 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     2341 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     2401 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     2461 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     2521 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
     2581 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     2641 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     2701 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     2761 ggaacgaaaa ctcacgttaa gggattttgg tcatgcattc taggtactaa aacaattcat
     2821 ccagtaaaat ataatatttt attttctccc aatcaggctt gatccccagt aagtcaaaaa
     2881 atagctcgac atactgttct tccccgatat cctccctgat cgaccggacg cagaaggcaa
     2941 tgtcatacca cttgtccgcc ctgccgcttc tcccaagatc aataaagcca cttactttgc
     3001 catctttcac aaagatgttg ctgtctccca ggtcgccgtg ggaaaagaca agttcctctt
     3061 cgggcttttc cgtctttaaa aaatcataca gctcgcgcgg atctttaaat ggagtgtctt
     3121 cttcccagtt ttcgcaatcc acatcggcca gatcgttatt cagtaagtaa tccaattcgg
     3181 ctaagcggct gtctaagcta ttcgtatagg gacaatccga tatgtcgatg gagtgaaaga
     3241 gcctgatgca ctccgcatac agctcgataa tcttttcagg gctttgttca tcttcatact
     3301 cttccgagca aaggacgcca tcggcctcac tcatgagcag attgctccag ccatcatgcc
     3361 gttcaaagtg caggaccttt ggaacaggca gctttccttc cagccatagc atcatgtcct
     3421 tttcccgttc cacatcatag gtggtccctt tataccggct gtccgtcatt tttaaatata
     3481 ggttttcatt ttctcccacc agcttatata ccttagcagg agacattcct tccgtatctt
     3541 ttacgcagcg gtatttttcg atcagttttt tcaattccgg tgatattctc attttagcca
     3601 tttattattt ccttcctctt ttctacagta tttaaagata ccccaagaag ctaattataa
     3661 caagacgaac tccaattcac tgttccttgc attctaaaac cttaaatacc agaaaacagc
     3721 tttttcaaag ttgttttcaa agttggcgta taacatagta tcgacggagc cgattttgaa
     3781 accgcggtga tcacaggcag caacgctctg tcatcgttac aatcaacatg ctaccctccg
     3841 cgagatcatc cgtgtttcaa acccggcagc ttagttgccg ttcttccgaa tagcatcggt
     3901 aacatgagca aagtctgccg ccttacaacg gctctcccgc tgacgccgtc ccggactgat
     3961 gggctgcctg tatcgagtgg tgattttgtg ccgagctgcc ggtcggggag ctgttggctg
     4021 gctggtggca ggatatattg tggtgtaaac aaattgacgc ttagacaact taataacaca
     4081 ttgcggacgt ttttaatgta ctgaattaac gccgaattaa ttcgagctcg gatctgataa
     4141 tttatttgaa aattcataag aaaagcaaac gttacatgaa ttgatgaaac aatacaaaga
     4201 cagataaagc cacgcacatt taggatattg gccgagatta ctgaatattg agtaagatca
     4261 cggaatttct gacaggagca tgtcttcaat tcagcccaaa tggcagttga aatactcaaa
     4321 ccgccccata tgcaggagcg gatcattcat tgtttgtttg gttgcctttg ccaacatggg
     4381 agtccaagat tctgcagtca aatctcggtg acgggcagga ccggacgggg cggtaccggc
     4441 aggctgaagt ccagctgcca gaaacccacg tcatgccagt tcccgtgctt gaagccggcc
     4501 gcccgcagca tgccgcgggg ggcatatccg agcgcctcgt gcatgcgcac gctcgggtcg
     4561 ttgggcagcc cgatgacagc gaccacgctc ttgaagccct gtgcctccag ggacttcagc
     4621 aggtgggtgt agagcgtgga gcccagtccc gtccgctggt ggcgggggga gacgtacacg
     4681 gtcgactcgg ccgtccagtc gtaggcgttg cgtgccttcc aggggcccgc gtaggcgatg
     4741 ccggcgacct cgccgtccac ctcggcgacg agccagggat agcgctcccg cagacggacg
     4801 aggtcgtccg tccactcctg cggttcctgc ggctcggtac ggaagttgac cgtgcttgtc
     4861 tcgatgtagt ggttgacgat ggtgcagacc gccggcatgt ccgcctcggt ggcacggcgg
     4921 atgtcggccg ggcgtcgttc tgggctcatc gattcgattt ggtgtatcga gattggttat
     4981 gaaattcaga tgctagtgta atgtattggt aatttgggaa gatataatag gaagcaaggc
     5041 tatttatcca tttctgaaaa ggcgaaatgg cgtcaccgcg agcgtcacgc gcattccgtt
     5101 cttgctgtaa agcgttgttt ggtacacttt tgactagcga ggcttggcgt gtcagcgtat
     5161 ctattcaaaa gtcgttaatg gctgcggatc aagaaaaagt tggaatagaa acagaatacc
     5221 cgcgaaattc aggcccggtt gccatgtcct acacgccgaa ataaacgacc aaattagtag
     5281 aaaaataaaa actgactcgg atacttacgt cacgtcttgc gcactgattt gaaaaatctc
     5341 agaattcatg gatcctaggt gagtctagag agttaattaa gaaaggcgga caggtatccg
     5401 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg gaaacgcctg
     5461 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     5521 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggatcaca acaaaaagcc
     5581 cgctcattag gcgggctaaa ttctcatgtt tgacagctta tcatcgataa gctttaatga
     5641 gttatcgaga ttttcaggag ctaaggaagc taaaatggag aaagaaatca ctggatatac
     5701 caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc
     5761 tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa
     5821 gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc
     5881 tcatccggag ttccgtatgg caatgaaaga cggtgagctg gtaatatggg atagtgttca
     5941 cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata
     6001 ccacgacgat ttccggcagt ttttacacat atattcgcaa gatgtggcgt gttacggtga
     6061 aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc
     6121 ctgggattgg agcttcagct gctcgagttc tatagtgtca cctaaatcgt atgtgtatga
     6181 tacataaggt tatgtattaa ttgtagccgc gttcttacgt cgacgtacca attacgcccc
     6241 gccctgtcac tcatcgcagt actgttgtaa ttcattaagc attctgccga catggaagcc
     6301 atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt
     6361 ataatatttg cccatggtga aaacgggggc gaagaagttg tccatattgg ccacgtttaa
     6421 atcaaaactg gtgaaactca cccagggatt ggctgagacg aaaaacatat tctcaataaa
     6481 ccctttaggg aaataggcca ggttttcacc gtaacacgcc acatcttgcg aatatatgtg
     6541 taaaaactgc cggaaatcgt cgtggtattc actccagagc gatgaaaacg tttcagtttg
     6601 ctcatggaaa acggtgtaac aagggtgaac actatcccat attaccagct caccgtcttt
     6661 cattgccata cggaactccg gatgagcatt catcaggcgg gcaagaatgt gaataaaggc
     6721 cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa tatccagctg
     6781 aacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat gttctttacg
     6841 atgccattgg gatatatcaa cggtggtata tccagtgatt tctttctcca ttttagcttc
     6901 cttagctcct gaaaatctcg ataactcatt aaagcttatc gatgataagc tgtcaaacat
     6961 gagaatttag cccgcctaat gagcgggctt tttgttgtga tccgcgttgc tggcgttttt
     7021 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     7081 aaacccgaca ggactataaa gataccaggc gtttcccccc tggaagctcc ctcgtgcgct
     7141 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttcttaat taagacccgg
     7201 gactagtccc tagagtcctg ctttaatgag atatgcgaga cgcctatgat cgcatgatat
     7261 ttgctttcaa ttctgttgtg cacgttgtaa aaaacctgag catgtgtagc tcagatcctt
     7321 accgccggtt tcggttcatt ctaatgaata tatcacccgt tactatcgta tttttatgaa
     7381 taatattctc cgttcaattt actgattgta ccctactact tatatgtaca atattaaaat
     7441 gaaaacaata tattgtgctg aataggttta tagcgacatc tatgatagag cgccacaata
     7501 acaaacaatt gcgttttatt attacaaatc caattttaaa aaaagcggca gaaccggtca
     7561 aacctaaaag actgattaca taaatcttat tcaaatttca aaagtgcccc aggggctagt
     7621 atctacgaca caccgagcgg cgaactaata acgctcactg aagggaactc cggttccccg
     7681 ccggcgcgca tgggtgagat tccttgaagt tgagtattgg ccgtccgctc taccgaaagt
     7741 tacgggcacc attcaacccg gtccagcacg gcggccgggt aaccgacttg ctgccccgag
     7801 aattatgcag catttttttg gtgtatgtgg gccccaaatg aagtgcaggt caaaccttga
     7861 cagtgacgac aaatcgttgg gcgggtccag ggcgaatttt gcgacaacat gtcgaggctc
     7921 agcaggacct gcaggcatgc aagcttggca ctggccgtcg ttttacaacg tcgtgactgg
     7981 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     8041 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     8101 gaatgctaga gcagcttgag cttggatcag attgtcgttt cccgccttca gtttaaacta
     8161 tcagtgtttg acaggatata ttggcgggta aacctaagag aaaagagcgt ttattagaat
     8221 aatcggatat ttaaaagggc gtgaaaaggt ttatccgttc gtccatttgt atgtgcatgc
     8281 caaccacagg gttcccctcg ggatcaaagt actttgatcc aacccctccg ctgctatagt
     8341 gcagtcggct tctgacgttc agtgcagccg tcttctgaaa acgacatgtc gcacaagtcc
     8401 taagttacgc gacaggctgc cgccctgccc ttttcctggc gttttcttgt cgcgtgtttt
     8461 agtcgcataa agtagaatac ttgcgactag aaccggagac attacgccat gaacaagagc
     8521 gccgccgctg gcctgctggg ctatgcccgc gtcagcaccg acgaccagga cttgaccaac
     8581 caacgggccg aactgcacgc ggccggctgc accaagctgt tttccgagaa gatcaccggc
     8641 accaggcgcg accgcccgga gctggccagg atgcttgacc acctacgccc tggcgacgtt
     8701 gtgacagtga ccaggctaga ccgcctggcc cgcagcaccc gcgacctact ggacattgcc
     8761 gagcgcatcc aggaggccgg cgcgggcctg cgtagcctgg cagagccgtg ggccgacacc
     8821 accacgccgg ccggccgcat ggtgttgacc gtgttcgccg gcattgccga gttcgagcgt
     8881 tccctaatca tcgaccgcac ccggagcggg cgcgaggccg ccaaggcccg aggcgtgaag
     8941 tttggccccc gccctaccct caccccggca cagatcgcgc acgcccgcga gctgatcgac
     9001 caggaaggcc gcaccgtgaa agaggcggct gcactgcttg gcgtgcatcg ctcgaccctg
     9061 taccgcgcac ttgagcgcag cgaggaagtg acgcccaccg aggccaggcg gcgcggtgcc
     9121 ttccgtgagg acgcattgac cgaggccgac gccctggcgg ccgccgagaa tgaacgccaa
     9181 gaggaacaag catgaaaccg caccaggacg gccaggacga accgtttttc attaccgaag
     9241 agatcgaggc ggagatgatc gcggccgggt acgtgttcga gccgcccgcg cacgtctcaa
     9301 ccgtgcggct gcatgaaatc ctggccggtt tgtctgatgc caagctggcg gcctggccgg
     9361 ccagcttggc cgctgaagaa accgagcgcc gccgtctaaa aaggtgatgt gtatttgagt
     9421 aaaacagctt gcgtcatgcg gtcgctgcgt atatgatgcg atgagtaaat aaacaaatac
     9481 gcaaggggaa cgcatgaagg ttatcgctgt acttaaccag aaaggcgggt caggcaagac
     9541 gaccatcgca acccatctag cccgcgccct gcaactcgcc ggggccgatg ttctgttagt
     9601 cgattccgat ccccagggca gtgcccgcga ttgggcggcc gtgcgggaag atcaaccgct
     9661 aaccgttgtc ggcatcgacc gcccgacgat tgaccgcgac gtgaaggcca tcggccggcg
     9721 cgacttcgta gtgatcgacg gagcgcccca ggcggcggac ttggctgtgt ccgcgatcaa
     9781 ggcagccgac ttcgtgctga ttccggtgca gccaagccct tacgacatat gggccaccgc
     9841 cgacctggtg gagctggtta agcagcgcat tgaggtcacg gatggaaggc tacaagcggc
     9901 ctttgtcgtg tcgcgggcga tcaaaggcac gcgcatcggc ggtgaggttg ccgaggcgct
     9961 ggccgggtac gagctgccca ttcttgagtc ccgtatcacg cagcgcgtga gctacccagg
    10021 cactgccgcc gccggcacaa ccgttcttga atcagaaccc gagggcgacg ctgcccgcga
    10081 ggtccaggcg ctggccgctg aaattaaatc aaaactcatt tgagttaatg aggtaaagag
    10141 aaaatgagca aaagcacaaa cacgctaagt gccggccgtc cgagcgcacg cagcagcaag
    10201 gctgcaacgt tggccagcct ggcagacacg ccagccatga agcgggtcaa ctttcagttg
    10261 ccggcggagg atcacaccaa gctgaagatg tacgcggtac gccaaggcaa gaccattacc
    10321 gagctgctat ctgaatacat cgcgcagcta ccagagtaaa tgagcaaatg aataaatgag
    10381 tagatgaatt ttagcggcta aaggaggcgg catggaaaat caa
//
