LOCUS       sequence-16722-e        1369 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1369)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1997
REFERENCE   2  (bases 1 to 1369)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: L3621
FEATURES             Location/Qualifiers
     source          1..1369
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             357..377
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(420..448)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 gctgcngtcg actctagagg atccccaatt cttgagactt ccactatctt ctctggcacc
       61 ctccactacg cgtaggcgtc tctcgcttcg tgtattcccg ggaagccggg ttcccgtctc
      121 tcccgccgct gccgctgccg cacacagctt tacacctcgt agaatcccca aagaggggcg
      181 tggcttgcgg gtgccaacat cctcctgccg aggaagaagc aggcactcat cactcgcatc
      241 atcaacctcg ggattggcca aaggacccaa aggtatgttt cgaatgatac taacataaca
      301 tagaacattt tcaggaggac ccttggaggg taccgagctc agaaaaaatg actgctccaa
      361 agaagaagcg taaggtaccg gtagaaaaaa tgagtaaagg agaagaactt ttcactggag
      421 ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtcagtg
      481 gagagggtga aggtgatgca acatacggaa aacttaccct taaatttatt tgcactactg
      541 gaaaactacc tgttccatgg gtaagtttaa acatatatat actaactaac cctgattatt
      601 taaattttca gccaacactt gtcactactt tcacttatgg tgttcaatgc ttctcgagat
      661 acccagatca tatgaaacgg catgactttt tcaagagtgc catgcccgaa ggttatgtac
      721 aggaaagaac tatatttttc aaagatgacg ggaactacaa gacacgtaag tttaaacagt
      781 tcggtactaa ctaaccatac atatttaaat tttcaggtgc tgaagtcaag tttgaaggtg
      841 atacccttgt taatagaatc gagttaaaag gtattgattt taaagaagat ggaaacattc
      901 ttggacacaa attggaatac aactataact cacacaatgt atacatcgca gcagacaaac
      961 naaagaatgg aatcaaagtt gtangtttta aacatgattt tactanctan ctaatctgat
     1021 ttaattttnc naactcnaat tagacccaca ttgaanagga gggtcactag cnacctttca
     1081 caatactcca ttggganggc ntgtctttnc nnaaaacatt actgncnnnn atntgccttn
     1141 aaaatccanc aaaanaaaac nntggncttc tganttganc ccgcnggaat acanggagga
     1201 gaatnnnnag nccgggggga aaacaaannc ccccaatgac cnnaaatncc ngttnccccc
     1261 nntgggaaaa anccntttta anncggnggn aaccggggtn caaaannctg annnccctnc
     1321 cggggaaaaa nacccccccc ccccgggnan anaaccnaat tnnnanttg
//
