LOCUS       sequence-167221-        1004 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Putidaredoxin fused to Pro5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Tan CY, Hirakawa H, Suzuki R, Haga T, Iwata F, Nagamune T
  TITLE     Immobilization of a Bacterial Cytochrome P450 Monooxygenase System 
            on a Solid Support.
  JOURNAL   Angew Chem Int Ed Engl. 2016 Oct 26. doi: 10.1002/anie.201608033.
  PUBMED    27781345
REFERENCE   2  (bases 1 to 1004)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Angew Chem 
            Int Ed Engl. 2016 Oct 26. doi: 10.1002/anie.201608033."
COMMENT     Sequence Label: pBsaPro5X
FEATURES             Location/Qualifiers
     source          1..1004
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(408..424)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(408..424)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(421..443)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    432..448
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(456..462,463..480,481..486))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    501..522
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 tctagaggat cggtctcgga tcgaattctc accactgacg gtccggaacg tcaacaacga
       61 taccgtccag ctccggggtc atgatgatct ggcaagacag acgagagttc ggtttcagtt
      121 ccgcggtaac agattccagc ataccgattt cacgttcgtt agcagccgga actttgtcgg
      181 tgaacgcttc gttcacgtac acgtggcagg tcgcgcaaga cgcagaacca ccgcagtcac
      241 caacgatgtc gtagatgccg ttagaaactg ccgcctgcat cagagaaaca ccgtccgcaa
      301 cgtccagttc gcgacgggta ccgtcgtgag aaacgtaaac aactttagac atagagccac
      361 cacctccggg cggcggtgga ggtggaggag accaattcgt aatcatggtc atagctgttt
      421 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
      481 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
      541 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
      601 gggagaggcg gtttgcgtat tggagcttgg cactgggcca agctgaattt ctgccattca
      661 tccgcttatt atcacttatt caggcgtagc aaccaggcgt ttaagggcac caataactgc
      721 cttaaaaaaa ttacgccccg ccctgccact catcgcagta ctgttgtaat tcattaagca
      781 ttctgccgac atggaagcca tcacaaacgg catgatgaac ctgaatcgcc agcggcatca
      841 gcaccttgtc gccttgcgta taatatttgc ccatggtgaa aacgggggcg aagaagttgt
      901 ccatattggc cacgtttaaa tcaaaactgg tgaaactcac ccagggattg gctgagacga
      961 aaaacatatt ctcaataaac cctttaggga aataggccag gttt
//
