LOCUS       sequence-167222-        3487 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses the human HMG-CoA reductase isoform 1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3487)
  TITLE     Genethon pCMV-SPORT6-hHMGCR1 plasmid
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3487)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCMV-SPORT6-hHMGCR1
FEATURES             Location/Qualifiers
     source          1..3487
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     enhancer        1..303
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        304..507
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     458..478
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     504..528
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     633..649
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     633..649
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        660..678
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     660..677
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    679..703
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2915..2926
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
       61 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
      121 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
      181 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
      241 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
      301 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
      361 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
      421 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
      481 cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc
      541 catccacgct gttttgacct ccatagaaga caccgggacc gatccagcct ccggactcta
      601 gcctaggccg cgggacggat aacaatttca cacaggaaac agctatgacc attaggccta
      661 tttaggtgac actatagaac aagtttgtac aaaaaagcag gctggtaccg gtccggaatt
      721 cccgggatcg actgcgttaa ctggagccag gctgagcgtc ggcgccgggg ttcggtggcc
      781 tctagtgaga tctggaggat ccaaggattc tgtagctaca atgttgtcaa gactttttcg
      841 aatgcatggc ctctttgtgg cctcccatcc ctgggaagtc atagtgggga cagtgacact
      901 gaccatctgc atgatgtcca tgaacatgtt tactggtaac aataagatct gtggttggaa
      961 ttatgaatgt ccaaagtttg aagaggatgt tttgagcagt gacattataa ttctgacaat
     1021 aacacgatgc atagccatcc tgtatattta cttccagttc cagaatttac gtcaacttgg
     1081 atcaaaatat attttgggta ttgctggcct tttcacaatt ttctcaagtt ttgtattcag
     1141 tacagttgtc attcacttct tagacaaaga attgacaggc ttgaatgaag ctttgccctt
     1201 tttcctactt ttgattgacc tttccagagc aagcacatta gcaaagtttg ccctcagttc
     1261 caactcacag gatgaagtaa gggaaaatat tgctcgtgga atggcaattt taggtcctac
     1321 gtttaccctc gatgctcttg ttgaatgtct tgtgattgga gttggtacca tgtcaggggt
     1381 acgtcagctt gaaattatgt gctgctttgg ctgcatgtca gttcttgcca actacttcgt
     1441 gttcatgact ttcttcccag cttgtgtgtc cttggtatta gagctttctc gggaaagccg
     1501 cgagggtcgt ccaatttggc agctcagcca ttttgcccga gttttagaag aagaagaaaa
     1561 taagccgaat cctgtaactc agagggtcaa gatgattatg tctctaggct tggttcttgt
     1621 tcatgctcac agtcgctgga tagctgatcc ttctcctcaa aacagtacag cagatacttc
     1681 taaggtttca ttaggactgg atgaaaatgt gtccaagaga attgaaccaa gtgtttccct
     1741 ctggcagttt tatctctcta aaatgatcag catggatatt gaacaagtta ttaccctaag
     1801 tttagctctc cttctggctg tcaagtacat cttctttgaa caaacagaga cagaatctac
     1861 actctcatta aaaaacccta tcacatctcc tgtagtgaca caaaagaaag tcccagacaa
     1921 ttgttgtaga cgtgaaccta tgctggtcag aaataaccag aaatgtgatt cagtagagga
     1981 agagacaggg ataaaccgag aaagaaaagt tgaggttata aaacccttag tggctgaaac
     2041 agatacccca aacagagcta catttgtggt tggtaactcc tccttactcg atacttcatc
     2101 agtactggtg acacaggaac ctgaaattga acttcccagg gaacctcggc ctaatgaaga
     2161 atgtctacag atacttggga atgcagagaa aggtgcaaaa ttccttagtg atgctgagat
     2221 catccagtta gtcaatgcta agcatatccc agcctacaag ttggaaactc tgatggaaac
     2281 tcatgagcgt ggtgtatcta ttcgccgaca gttactttcc aagaagcttt cagaaccttc
     2341 ttctctccag tacctacctt acagggatta taattactcc ttggtgatgg gagcttgttg
     2401 tgagaatgtt attggatata tgcccatccc tgttggagtg gcaggacccc tttgcttaga
     2461 tgaaaaagaa tttcaggttc caatggcaac aacagaaggt tgtcttgtgg ccagcaccaa
     2521 tagaggctgc agagcaatag gtcttggtgg aggtgccagc agccgagtcc ttgcagatgg
     2581 gatgactcgt ggcccagttg tgcgtcttcc acgtgcttgt gactctgcag aagtgaaagc
     2641 ctggctcgaa acatctgaag ggttcgcagt gataaaggag gcatttgaca gcactagcag
     2701 atttgcacgt ctacagaaac ttcatacaag tatagctgga cgcaaccttt atatccgttt
     2761 ccagtccagg tcaggggatg ccatggggat gaacatgatt tcaaagggta cagagaaagc
     2821 actttcaaaa cttcacgagt atttccctga aatgcagatt ctagccgtta gtggtaacta
     2881 ttgtactgac aagaaacctg ctgctataaa ttggatagag ggaagaggaa aatctgttgt
     2941 ttgtgaagct gtcattccag ccaaggttgt cagagaagta ttaaagacta ccacagaggc
     3001 tatgattgag gtcaacatta acaagaattt agtgggctct gccatggctg ggagcatagg
     3061 aggctacaac gcccatgcag caaacattgt caccgccatc tacattgcct gtggacagga
     3121 tgcagcacag aatgttggta gttcaaactg tattacttta atggaagcaa gtggtcccac
     3181 aaatgaagat ttatatatca gctgcaccat gccatctata gagataggaa cggtgggtgg
     3241 tgggaccaac ctactacctc agcaagcctg tttgcagatg ctaggtgttc aaggagcatg
     3301 caaagataat cctggggaaa atgcccggca gcttgcccga attgtgtgtg ggaccgtaat
     3361 ggctggggaa ttgtcactta tggcagcatt ggcagcagga catcttgtca aaagtcacat
     3421 gattcacaac aggtcgaaga tcaatttaca agacctccaa ggagcttgca ccaagaagac
     3481 agcctga
//
