LOCUS       sequence-167233-       11550 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  target  miRNA1886.3  for destruction in Arabidopsis thaliana.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11550)
  AUTHORS   Tang G, Yan J, Gu Y, Qiao M, Fan R, Mao Y, Tang X
  TITLE     Construction of short tandem target mimic (STTM) to block the 
            functions of plant and animal microRNAs.
  JOURNAL   Methods. 2012 Oct;58(2):118-25. doi: 10.1016/j.ymeth.2012.10.006. 
            Epub 2012 Oct 23.
  PUBMED    23098881
REFERENCE   2  (bases 1 to 11550)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ymeth.2012.10.006"; journalName: "Methods"; date: 
            "2012-10"; volume: "58"; issue: "2"; pages: "118-25"
COMMENT     Sequence Label: pFGC5941-PacI-ath-STTM1886.3
FEATURES             Location/Qualifiers
     source          1..11550
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..346
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     289..309
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     polyA_signal    514..690
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(1252..1270)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1253..1270)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     1909..1928
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(2031..2036,2037..2053,2054..2059))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     promoter        2334..2714
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     CDS             2720..3271
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     terminator      3281..3533
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     misc_feature    3619..3643
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             4068..4862
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      4949..5537
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5691..5708
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    5723..5863
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(5789..5808)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5949..5971
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      6207..6401
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(6467..7540)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(7969..8598)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    9899..9923
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     10111..10133
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10125..10142
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10126..10142
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(10167..10874)
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     primer_bind     11125..11144
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        11125..11143
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tgagactttt caacaaaggg taatttcggg aaacctcctc ggattccatt gcccagctat
       61 ctgtcacctc atcgaaagga cagtagaaaa ggaaggtggc tcctacaaat gccatcattg
      121 cgataaagga aaggctatca ttcaagatgc ctctgccgac agtggtccca aagatggacc
      181 cccacccacg aggagcatcg tggaaaaaga agacgtccca accacgtctt caaagcaagt
      241 ggattgatgt gacatctcca ctgacataag ggatgacgca caaccccact atccttcgca
      301 agacccttcc tctatataag gaagttcatt tcatttggag aggacagccc aagcttagta
      361 agatgaactg atctttaatt gttgttgttg ttatggtcta atttgccatt taaatatggt
      421 ctaaagaaga agaatagtaa gatgaactga tctttaattg aattcggtac gctgaaatca
      481 ccagtctctc tctacaaatc tatctctctc tattttctcc ataaataatg tgtgagtagt
      541 ttcccgataa gggaaattag ggttcttata gggtttcgct catgtgttga gcatataaga
      601 aacccttagt atgtatttgt atttgtaaaa tacttctatc aataaaattt ctaattccta
      661 aaaccaaaat ccagtactaa aatccagatc tcctaaagtc cctatagatc tttgtcgtga
      721 atataaacca gacacgagac gactagacct ggagcccaga cgccgttcga agctagaagt
      781 accgcttagg caggaggccg ttagggaaaa gatgctaagg cagggttggt tacgttgact
      841 cccccgtagg tttggtttaa atatgatgaa gtggacggaa ggaaggagga agacaaggaa
      901 ggataaggct gcaggccctg tgcaaggtaa gaagatggaa atttgataga ggtacgctac
      961 tatacttata ctatacgcta agggaatgat tgtatttata ccctataccc cctaataacc
     1021 ccttatcaat ttaagaaata atccgcataa gcccccgctt aaaaattggt atcagagcca
     1081 tgaataggtc tatgaccaaa actcaagagg ataaaacctc accaaaatac gaaagagtcc
     1141 ttaactctaa agataaaaga tctttcaaga tcaaaactag ttccctcaca ccggtgacgg
     1201 ggatcgcatg cgacctctag accatcacat tggagcttca gctgctcgag ttctatagtg
     1261 tcacctaaat cgtatgcgta tgatacataa ggttatgcat taattgtagc cgcgttctta
     1321 cgtcgacgta ccaattacgc cccgccctgt cactcatcgc agtactgttg taattcatta
     1381 agcattctgc cgacatggaa gccatcacaa acggcatgat gaacctgaat cgccagcggc
     1441 atcagcacct tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag
     1501 ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg attggctgag
     1561 acgaaaaaca tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac
     1621 gccacatctt gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag
     1681 agcgatgaaa acgcttccgt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc
     1741 catattacca gctcaccgtc tttcactgcc atacggaact ccggatgagc attcatcagg
     1801 cgggcaagaa tgtgaataaa ggccggataa aacttgtgct tatttttctt tacggtcttt
     1861 aaaaaggccg taatatccag ctgaacggtc tggttatagg tacattgagc aactgactga
     1921 aatgcctcaa aatgttcttt acgatgccat tgggatatat caacggtggt atatccagtg
     1981 atttctttct ccattttagc ttccttagct cctgaaaatc tcgataactc attaaagctt
     2041 atcgatgata agctgtcaaa catgagaatt tagcccgcct aatgagcggg ctttttgttg
     2101 tgatccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     2161 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     2221 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     2281 cgcctttctt aattaactct ctagactcac ctaggatcca tgaattctga gatttttcaa
     2341 atcagtgcgc aagacgtgac gtaagtatcc gagtcagttt ttatttttct actaatttgg
     2401 tcgtttattt cggcgtgtag gacatggcaa ccgggcctga atttcgcggg tattctgttt
     2461 ctattccaac tttttcttga tccgcagcca ttaacgactt ttgaatagat acgctgacac
     2521 gccaagcctc gctagtcaaa agtgtaccaa acaacgcttt acagcaagaa cggaatgcgc
     2581 gtgacgctcg cggtgacgcc atttcgcctt ttcagaaatg gataaatagc cttgcttcct
     2641 attatatctt cccaaattac caatacatta cactagcatc tgaatttcat aaccaatctc
     2701 gatacaccaa atcgaatcga tgagcccaga acgacgcccg gccgacatcc gccgtgccac
     2761 cgaggcggac atgccggcgg tctgcaccat cgtcaaccac tacatcgaga caagcacggt
     2821 caacttccgt accgagccgc aggaaccgca ggagtggacg gacgacctcg tccgtctgcg
     2881 ggagcgctat ccctggctcg tcgccgaggt ggacggcgag gtcgccggca tcgcctacgc
     2941 gggcccctgg aaggcacgca acgcctacga ctggacggcc gagtcgaccg tgtacgtctc
     3001 cccccgccac cagcggacgg gactgggctc cacgctctac acccacctgc tgaagtccct
     3061 ggaggcacag ggcttcaaga gcgtggtcgc tgtcatcggg ctgcccaacg acccgagcgt
     3121 gcgcatgcac gaggcgctcg gatatgcccc ccgcggcatg ctgcgggcgg ccggcttcaa
     3181 gcacgggaac tggcatgacg tgggtttctg gcagctggac ttcagcctgc cggtaccgcc
     3241 ccgtccggtc ctgcccgtca ccgagatttg actgcagaat cttggactcc catgttggca
     3301 aaggcaacca aacaaacaat gaatgatccg ctcctgcata tggggcggtt tgagtatttc
     3361 aactgccatt tgggctgaat tgaagacatg ctcctgtcag aaattccgtg atcttactca
     3421 atattcagta atctcggcca atatcctaaa tgtgcgtggc tttatctgtc tttgtattgt
     3481 ttcatcaatt catgtaacgt ttgcttttct tatgaatttt caaataaatt atcagatccg
     3541 agctcgaatt aattcggcgt taattcagta cattaaaaac gtccgcaatg tgttattaag
     3601 ttgtctaagc gtcaatttgt ttacaccaca atatatcctg ccaccagcca gccaacagct
     3661 ccccgaccgg cagctcggca caaaatcacc actcgataca ggcagcccat cagtccggga
     3721 cggcgtcagc gggagagccg ttgtaaggcg gcagactttg ctcatgttac cgatgctatt
     3781 cggaagaacg gcaactaagc tgccgggttt gaaacacgga tgatctcgcg gagggtagca
     3841 tgttgattgt aacgatgaca gagcgttgct gcctgtgatc accgcggttt caaaatcggc
     3901 tccgtcgata ctatgttata cgccaacttt gaaaacaact ttgaaaaagc tgttttctgg
     3961 tatttaaggt tttagaatgc aaggaacagt gaattggagt tcgtcttgtt ataattagct
     4021 tcttggggta tctttaaata ctgtagaaaa gaggaaggaa ataataaatg gctaaaatga
     4081 gaatatcacc ggaattgaaa aaactgatcg aaaaataccg ctgcgtaaaa gatacggaag
     4141 gaatgtctcc tgctaaggta tataagctgg tgggagaaaa tgaaaaccta tatttaaaaa
     4201 tgacggacag ccggtataaa gggaccacct atgatgtgga acgggaaaag gacatgatgc
     4261 tatggctgga aggaaagctg cctgttccaa aggtcctgca ctttgaacgg catgatggct
     4321 ggagcaatct gctcatgagt gaggccgatg gcgtcctttg ctcggaagag tatgaagatg
     4381 aacaaagccc tgaaaagatt atcgagctgt atgcggagtg catcaggctc tttcactcca
     4441 tcgacatatc ggattgtccc tatacgaata gcttagacag ccgcttagcc gaattggatt
     4501 acttactgaa taacgatctg gccgatgtgg attgcgaaaa ctgggaagaa gacactccat
     4561 ttaaagatcc gcgcgagctg tatgattttt taaagacgga aaagcccgaa gaggaacttg
     4621 tcttttccca cggcgacctg ggagacagca acatctttgt gaaagatggc aaagtaagtg
     4681 gctttattga tcttgggaga agcggcaggg cggacaagtg gtatgacatt gccttctgcg
     4741 tccggtcgat cagggaggat atcggggaag aacagtatgt cgagctattt tttgacttac
     4801 tggggatcaa gcctgattgg gagaaaataa aatattatat tttactggat gaattgtttt
     4861 agtacctaga atgcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     4921 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     4981 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     5041 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
     5101 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     5161 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     5221 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     5281 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     5341 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     5401 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     5461 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     5521 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     5581 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     5641 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     5701 tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
     5761 tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     5821 agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac
     5881 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
     5941 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
     6001 gcagggtgcc ttgatgtggg cgccggcggt cgagtggcga cggcgcggct tgtccgcgcc
     6061 ctggtagatt gcctggccgt aggccagcca tttttgagcg gccagcggcc gcgataggcc
     6121 gacgcgaagc ggcggggcgt agggagcgca gcgaccgaag ggtaggcgct ttttgcagct
     6181 cttcggctgt gcgctggcca gacagttatg cacaggccag gcgggtttta agagttttaa
     6241 taagttttaa agagttttag gcggaaaaat cgcctttttt ctcttttata tcagtcactt
     6301 acatgtgtga ccggttccca atgtacggct ttgggttccc aatgtacggg ttccggttcc
     6361 caatgtacgg ctttgggttc ccaatgtacg tgctatccac aggaaagaga ccttttcgac
     6421 ctttttcccc tgctagggca atttgcccta gcatctgctc cgtacattag gaaccggcgg
     6481 atgcttcgcc ctcgatcagg ttgcggtagc gcatgactag gatcgggcca gcctgccccg
     6541 cctcctcctt caaatcgtac tccggcaggt catttgaccc gatcagcttg cgcacggtga
     6601 aacagaactt cttgaactct ccggcgctgc cactgcgttc gtagatcgtc ttgaacaacc
     6661 atctggcttc tgccttgcct gcggcgcggc gtgccaggcg gtagagaaaa cggccgatgc
     6721 cgggatcgat caaaaagtaa tcggggtgaa ccgtcagcac gtccgggttc ttgccttctg
     6781 tgatctcgcg gtacatccaa tcagctagct cgatctcgat gtactccggc cgcccggttt
     6841 cgctctttac gatcttgtag cggctaatca aggcttcacc ctcggatacc gtcaccaggc
     6901 ggccgttctt ggccttcttc gtacgctgca tggcaacgtg cgtggtgttt aaccgaatgc
     6961 aggtttctac caggtcgtct ttctgctttc cgccatcggc tcgccggcag aacttgagta
     7021 cgtccgcaac gtgtggacgg aacacgcggc cgggcttgtc tcccttccct tcccggtatc
     7081 ggttcatgga ttcggttaga tgggaaaccg ccatcagtac caggtcgtaa tcccacacac
     7141 tggccatgcc ggccggccct gcggaaacct ctacgtgccc gtctggaagc tcgtagcgga
     7201 tcacctcgcc agctcgtcgg tcacgcttcg acagacggaa aacggccacg tccatgatgc
     7261 tgcgactatc gcgggtgccc acgtcataga gcatcggaac gaaaaaatct ggttgctcgt
     7321 cgcccttggg cggcttccta atcgacggcg caccggctgc cggcggttgc cgggattctt
     7381 tgcggattcg atcagcggcc gcttgccacg attcaccggg gcgtgcttct gcctcgatgc
     7441 gttgccgctg ggcggcctgc gcggccttca acttctccac caggtcatca cccagcgccg
     7501 cgccgatttg taccgggccg gatggtttgc gaccgctcac gccgattcct cgggcttggg
     7561 ggttccagtg ccattgcagg gccggcaggc aacccagccg cttacgcctg gccaaccgcc
     7621 cgttcctcca cacatggggc attccacggc gtcggtgcct ggttgttctt gattttccat
     7681 gccgcctcct ttagccgcta aaattcatct actcatttat tcatttgctc atttactctg
     7741 gtagctgcgc gatgtattca gatagcagct cggtaatggt cttgccttgg cgtaccgcgt
     7801 acatcttcag cttggtgtga tcctccgccg gcaactgaaa gttgacccgc ttcatggctg
     7861 gcgtgtctgc caggctggcc aacgttgcag ccttgctgct gcgtgcgctc ggacggccgg
     7921 cacttagcgt gtttgtgctt ttgctcattt tctctttacc tcattaactc aaatgagttt
     7981 tgatttaatt tcagcggcca gcgcctggac ctcgcgggca gcgtcgccct cgggttctga
     8041 ttcaagaacg gttgtgccgg cggcggcagt gcctgggtag ctcacgcgct gcgtgatacg
     8101 ggactcaaga atgggcagct cgtacccggc cagcgcctcg gcaacctcac cgccgatgcg
     8161 cgtgcctttg atcgcccgcg acacgacaaa ggccgcttgt agccttccat ccgtgacctc
     8221 aatgcgctgc ttaaccagct ccaccaggtc ggcggtggcc catatgtcgt aagggcttgg
     8281 ctgcaccgga atcagcacga agtcggctgc cttgatcgcg gacacagcca agtccgccgc
     8341 ctggggcgct ccgtcgatca ctacgaagtc gcgccggccg atggccttca cgtcgcggtc
     8401 aatcgtcggg cggtcgatgc cgacaacggt tagcggttga tcttcccgca cggccgccca
     8461 atcgcgggca ctgccctggg gatcggaatc gactaacaga acatcggccc cggcgagttg
     8521 cagggcgcgg gctagatggg ttgcgatggt cgtcttgcct gacccgcctt tctggttaag
     8581 tacagcgata accttcatgc gttccccttg cgtatttgtt tatttactca tcgcatcata
     8641 tacgcagcga ccgcatgacg caagctgttt tactcaaata cacatcacct ttttagacgg
     8701 cggcgctcgg tttcttcagc ggccaagctg gccggccagg ccgccagctt ggcatcagac
     8761 aaaccggcca ggatttcatg cagccgcacg gttgagacgt gcgcgggcgg ctcgaacacg
     8821 tacccggccg cgatcatctc cgcctcgatc tcttcggtaa tgaaaaacgg ttcgtcctgg
     8881 ccgtcctggt gcggtttcat gcttgttcct cttggcgttc attctcggcg gccgccaggg
     8941 cgtcggcctc ggtcaatgcg tcctcacgga aggcaccgcg ccgcctggcc tcggtgggcg
     9001 tcacttcctc gctgcgctca agtgcgcggt acagggtcga gcgatgcacg ccaagcagtg
     9061 cagccgcctc tttcacggtg cggccttcct ggtcgatcag ctcgcgggcg tgcgcgatct
     9121 gtgccggggt gagggtaggg cgggggccaa acttcacgcc tcgggccttg gcggcctcgc
     9181 gcccgctccg ggtgcggtcg atgattaggg aacgctcgaa ctcggcaatg ccggcgaaca
     9241 cggtcaacac catgcggccg gccggcgtgg tggtgtcggc ccacggctct gccaggctac
     9301 gcaggcccgc gccggcctcc tggatgcgct cggcaatgtc cagtaggtcg cgggtgctgc
     9361 gggccaggcg gtctagcctg gtcactgtca caacgtcgcc agggcgtagg tggtcaagca
     9421 tcctggccag ctccgggcgg tcgcgcctgg tgccggtgat cttctcggaa aacagcttgg
     9481 tgcagccggc cgcgtgcagt tcggcccgtt ggttggtcaa gtcctggtcg tcggtgctga
     9541 cgcgggcata gcccagcagg ccagcggcgg cgctcttgtt catggcgtaa tgtctccggt
     9601 tctagtcgca agtattctac tttatgcgac taaaacacgc gacaagaaaa cgccaggaaa
     9661 agggcagggc ggcagcctgt cgcgtaactt aggacttgtg cgacatgtcg ttttcagaag
     9721 acggctgcac tgaacgtcag aagccgactg cactatagca gcggaggggt tggatcaaag
     9781 tactttgatc ccgaggggaa ccctgtggtt ggcatgcaca tacaaatgga cgaacggata
     9841 aaccttttca cgccctttta aatatccgat tattctaata aacgctcttt tctcttaggt
     9901 ttacccgcca atatatcctg tcaaacactg atagtttaaa ctgaaggcgg gaaacgacaa
     9961 tctgatccaa gctcaagctg ctctagcatt cgccattcag gctgcgcaac tgttgggaag
    10021 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
    10081 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
    10141 gtgccaagct tgcatgcctg caggtcctgc tgagcctcga catgttgtcg caaaattcgc
    10201 cctggacccg cccaacgatt tgtcgtcact gtcaaggttt gacctgcact tcatttgggg
    10261 cccacataca ccaaaaaaat gctgcataat tctcggggca gcaagtcggt tacccggccg
    10321 ccgtgctgga ccgggttgaa tggtgcccgt aactttcggt agagcggacg gccaatactc
    10381 aacttcaagg aatctcaccc atgcgcgccg gcggggaacc ggagttccct tcagtgagcg
    10441 ttattagttc gccgctcggt gtgtcgtaga tactagcccc tggggcactt ttgaaatttg
    10501 aataagattt atgtaatcag tcttttaggt ttgaccggtt ctgccgcttt ttttaaaatt
    10561 ggatttgtaa taataaaacg caattgtttg ttattgtggc gctctatcat agatgtcgct
    10621 ataaacctat tcagcacaat atattgtttt cattttaata ttgtacatat aagtagtagg
    10681 gtacaatcag taaattgaac ggagaatatt attcataaaa atacgatagt aacgggtgat
    10741 atattcatta gaatgaaccg aaaccggcgg taaggatctg agctacacat gctcaggttt
    10801 tttacaacgt gcacaacaga attgaaagca aatatcatgc gatcataggc gtctcgcata
    10861 tctcattaaa gcaggactct agggactagt cccgggtctt aattaagttt gcaagcagca
    10921 gattacgcgg atacctgtcc gcctttctta attaagaaag gcggacaggt atccggtaat
    10981 ctgctgcttg caaattacgc gcagaaaaaa aggatctcaa ggagatcctt tggatcacaa
    11041 aaaaaaagcc cgctcattag gcgggttaaa ttcccgggta ctgcagccga ttcattaatg
    11101 caggttaacc tggcttatcg aaattaatac gactcactat agggagaccg gcagatctga
    11161 tccctactcc aaaaatgtca aagatacagt ctcagaagac caaagggcta ttgagacttt
    11221 tcaacaaagg gtaatttcgg gaaacctcct cggattccat tgcccagcta tctgtcactt
    11281 catcgaaagg acagtggaaa aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg
    11341 aaaggctatc attcaagatg cctctgccga cagtggtccc aaagatggac ccccacccac
    11401 gaggagcatc gtgggaaaag aagacgttcc aaccacgtct tcaaagcaag tggattgatg
    11461 tgacatctcc actgacgtaa gggatgacgc acaatcccac ccctactcca aaaatgtcaa
    11521 agatactgtc tcagaagacc aaagggctat
//
