LOCUS       sequence-167234-         796 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human codon-optimized AsCpf1 protein and blasticidin 
            resistance from EFS promoter. Lentiviral backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Kim HK, Song M, Lee J, Menon AV, Jung S, Kang YM, Choi JW, Woo E, 
            Koh HC, Nam JW, Kim H
  TITLE     In vivo high-throughput profiling of CRISPR-Cpf1 activity.
  JOURNAL   Nat Methods. 2016 Dec 19. doi: 10.1038/nmeth.4104.
  PUBMED    27992409
REFERENCE   2  (bases 1 to 796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Dec 19. doi: 10.1038/nmeth.4104."
COMMENT     Sequence Label: Lenti-AsCpf1-Blast
FEATURES             Location/Qualifiers
     source          1..796
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt
       61 ttcgcaacgg gtttgccgcc agaacacagg accggttcta gcgtttaaac ttaagcttgg
      121 taccgccacc atgacacagt tcgagggctt taccaacctg tatcaggtga gcaagacact
      181 gcggtttgag ctgatcccac agggcaagac cctgaagcac atccaggagc agggcttcat
      241 cgaggaggac aaggcccgca atgatcacta caaggagctg aagcccatca tcgatcggat
      301 ctacaagacc tatgccgacc agtgcctgca gctggtgcag ctggattggg agaacctgag
      361 cgccgccatc gactcctata gaaaggagaa aaccgaggag acaaggaacg ccctgatcga
      421 ggagcaggcc acatatcgca atgccatcca cgactacttc atcggccgga cagacaacct
      481 gaccgatgcc atcaataaga gacacgccga gatctacaag ggcctgttca aggccgagct
      541 gtttaatggc aaggtgctga agcagctggg caccgtgacc acaaccgagc acgagaacgc
      601 cctgctgcgg agcttcgaca agtttacaac ctacttctcc ggcttttatg agaacaggaa
      661 gaacgtgttc agcgccgagg atatcagcac agccatccca caccgcatcg tgcaggacaa
      721 cttccccaag tttaaggaga attgtcacat cttcacacgc ctgatcaccg ccgtgcccag
      781 cctgcgggag cacttt
//
