LOCUS       sequence-167236-         858 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human codon-optimized AsCpf1 protein and blasticidin 
            resistance from EFS promoter. Lentiviral backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Kim HK, Song M, Lee J, Menon AV, Jung S, Kang YM, Choi JW, Woo E, 
            Koh HC, Nam JW, Kim H
  TITLE     In vivo high-throughput profiling of CRISPR-Cpf1 activity.
  JOURNAL   Nat Methods. 2016 Dec 19. doi: 10.1038/nmeth.4104.
  PUBMED    27992409
REFERENCE   2  (bases 1 to 858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Dec 19. doi: 10.1038/nmeth.4104."
COMMENT     Sequence Label: Lenti-AsCpf1-Blast
FEATURES             Location/Qualifiers
     source          1..858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(65..463)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             complement(464..520)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(530..577)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     regulatory      849..858
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cagtcaatct ttcacaaatt ttgtaatcca gaggttgatt accgataagc ttgatatcga
       61 attcttagcc ctcccacaca taaccagagg gcagcaattc acgaatccca actgccgtcg
      121 gctgtccatc actgtccttc actatggctt tgatcccagg atgcagatcg agaagcacct
      181 gtcggcaccg tccgcagggg ctcaagatgc ccctgttctc atttccgatc gcgacgatac
      241 aagtcaggtt gccagctgcc gcagcagcag cagtgcccag caccacgagt tctgcacaag
      301 gtcccccagt aaaatgatat acattgacac cagtgaagat gcggccgtcg ctagagagag
      361 ctgcgctggc gacgctgtag tcttcagaga tggggatgct gttgattgta gccgttgctc
      421 tttcaatgag ggtggattct tcttgagaca aaggcttggc catcggtcca ggattctctt
      481 cgacatctcc ggcttgtttc agcagagaga agtttgttgc gccggatccc tttttctttt
      541 ttgcctggcc ggcctttttc gtggccgccg gccttttgtt gcgcagctcc tggatgtagg
      601 ccagccagtc ctgattggag atgccgttct gcagcttcag atccttgctc tccttcaggt
      661 gattcagcag cagctggccc ttcagggcga tgtggtaggc gccattggca tcggcgtcca
      721 tgggccactc tgggttctga aaccgggagt cgaagcacac gccattcaga tcgcgcacgg
      781 ggctgttgat atagtcctcg cctgtggcgg cattggagtt ccgcatctgc agcacgctgc
      841 ggatcagggc caccatgg
//
