LOCUS       sequence-167237-         964 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human codon-optimized LbCpf1 protein and blasticidin 
            resistance from EFS promoter. Lentiviral backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 964)
  AUTHORS   Kim HK, Song M, Lee J, Menon AV, Jung S, Kang YM, Choi JW, Woo E, 
            Koh HC, Nam JW, Kim H
  TITLE     In vivo high-throughput profiling of CRISPR-Cpf1 activity.
  JOURNAL   Nat Methods. 2016 Dec 19. doi: 10.1038/nmeth.4104.
  PUBMED    27992409
REFERENCE   2  (bases 1 to 964)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Dec 19. doi: 10.1038/nmeth.4104."
COMMENT     Sequence Label: Lenti-LbCpf1-Blast
FEATURES             Location/Qualifiers
     source          1..964
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct
       61 ttttcgcaac gggtttgccg ccagaacaca ggaccggttc tagcgtttaa acttaagctt
      121 ggtaccgcca ccatgagcaa gctggagaag tttacaaact gctactccct gtctaagacc
      181 ctgaggttca aggccatccc tgtgggcaag acccaggaga acatcgacaa taagcggctg
      241 ctggtggagg acgagaagag agccgaggat tataagggcg tgaagaagct gctggatcgc
      301 tactatctgt cttttatcaa cgacgtgctg cacagcatca agctgaagaa tctgaacaat
      361 tacatcagcc tgttccggaa gaaaaccaga accgagaagg agaataagga gctggagaac
      421 ctggagatca atctgcggaa ggagatcgcc aaggccttca agggcaacga gggctacaag
      481 tccctgttta agaaggatat catcgagaca atcctgccag agttcctgga cgataaggac
      541 gagatcgccc tggtgaacag cttcaatggc tttaccacag ccttcaccgg cttctttgat
      601 aacagagaga atatgttttc cgaggaggcc aagagcacat ccatcgcctt caggtgtatc
      661 aacgagaatc tgacccgcta catctctaat atggacatct tcgagaaggt ggacgccatc
      721 tttgataagc acgaggtgca ggagatcaag gagaagatcc tgaacagcga ctatgatgtg
      781 gaggatttct ttgagggcga gttctttaac tttgtgctga cacaggaggg catcgacgtg
      841 tataacgcca tcatcggcgg cttcgtgacc gagagcggcg agaagatcaa gggcctgaac
      901 gagtacatca acctgtataa tcagaaaacc aagcagaagc tgcctaagtt taagccactg
      961 tata
//
