LOCUS       sequence-167239-       11619 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  target  miRNA2111  for destruction in Arabidopsis thaliana.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11619)
  AUTHORS   Tang G, Yan J, Gu Y, Qiao M, Fan R, Mao Y, Tang X
  TITLE     Construction of short tandem target mimic (STTM) to block the 
            functions of plant and animal microRNAs.
  JOURNAL   Methods. 2012 Oct;58(2):118-25. doi: 10.1016/j.ymeth.2012.10.006. 
            Epub 2012 Oct 23.
  PUBMED    23098881
REFERENCE   2  (bases 1 to 11619)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ymeth.2012.10.006"; journalName: "Methods"; date: 
            "2012-10"; volume: "58"; issue: "2"; pages: "118-25"
COMMENT     Sequence Label: pFGC5941-PacI-ath-STTM2111a-5P/b-5P
FEATURES             Location/Qualifiers
     source          1..11619
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..346
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     289..309
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     polyA_signal    655..831
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(1393..1411)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1394..1411)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     2050..2069
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(2172..2177,2178..2194,2195..2200))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     promoter        2475..2855
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     CDS             2861..3412
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     terminator      3422..3674
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     misc_feature    3760..3784
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             4209..5003
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      5090..5678
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5832..5849
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    5864..6004
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(5930..5949)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6090..6112
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      6348..6542
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(6608..7681)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(8110..8739)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    10040..10064
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     10252..10274
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10266..10283
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10267..10283
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(10308..11015)
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     primer_bind     11194..11213
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        11194..11212
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tgagactttt caacaaaggg taatttcggg aaacctcctc ggattccatt gcccagctat
       61 ctgtcacctc atcgaaagga cagtagaaaa ggaaggtggc tcctacaaat gccatcattg
      121 cgataaagga aaggctatca ttcaagatgc ctctgccgac agtggtccca aagatggacc
      181 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt cagagcaagt
      241 ggattgatgt gacatctcca ctgacataag ggatgacgca caaccccact atccttcgca
      301 agacccttcc tctatataag gaagttcatt tcatttggag aggacagccc aagctttaaa
      361 cctcaggcta atgcagatta gttgttgttg ttatggtcta atttaaatat ggtctaaaga
      421 agaagaatta aacctcaggc taatgcagat tagaattcgg tacgctgaaa tcaccaggcc
      481 atttggagag gacagcccaa gctttaaacc tcaggctaat gcagattagt tgttgttgtt
      541 atggtctaat ttaaatatgg tctaaagaag aagaattaaa cctcaggcta atgcagatta
      601 gaattcggta cgctgaaatc accagtctct ctctacaaat ctatctctct ctattttctc
      661 cataaataat gtgtgagtag tttcccgata agggaaatta gggttcttat agggtttcgc
      721 tcatgtgttg agcatataag aaacccttag tatgtatttg tatttgtaaa atacttctat
      781 caataaaatt tctaattcct aaaaccaaaa tccagtacta aaatccagat ctcctaaagt
      841 ccctatagat ctttgtcgtg aatataaacc agacacgaga cgactagacc tggagcccag
      901 acgccgttcg aagctagaag taccgcttag gcaggaggcc gttagggaaa agatgctaag
      961 gcagggttgg ttacgttgac tcccccgtag gtttggttta aatatgatga agtggacggt
     1021 aggaaggagg aagacaagga aggataaggt tgcaggccct gtgcaaggta agaagatgga
     1081 aatttgatag aggtacgcta ctatacttat actatacgct aagggaatga ttgtatttat
     1141 accctatacc ccctaataac cccttatcaa tttaagaaat aatccgcata agcccccgct
     1201 taaaaattgg tatcagagcc atgaataggt ctatgaccaa aactcaagag gataaaacct
     1261 caccaaaata cgagagagtc cctaactcta aagataaaag atctttcaag atcaaaacta
     1321 gttccctcac accggtgacg gggatcgcat gcgacctcta gaccatcaca ttggagcttc
     1381 agctgctcga gttctatagt gtcacctaaa tcgtatgtgt atgatacata aggttatgta
     1441 ttaattgtag ccgcgttctt acgtcgacgt accaattacg ccccgccctg tcactcatcg
     1501 cagtactgtt gtaattcatt aagcattctg ccgacatgga agccatcaca aacggcatga
     1561 tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt gcgtataata tttgcccatg
     1621 gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa actggtgaaa
     1681 ttcacccagg gattggctga gacgaaaaac atattctcaa taaacccttt agggaaatag
     1741 gccaggtttt caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa
     1801 tcgtcgcggt attcactcca gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg
     1861 taacaagggt gaacactatc ccatattacc agctcaccgt ctttcattgc catacggaac
     1921 tccggatgag cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc
     1981 ttatttttct ttacggtctt taaaaaggcc gtaatatcca gctgaacggt ctggttatag
     2041 gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata
     2101 tcaacggtgg tatatccagt gatttctttc tccattttag cttccttagc tcctgaaaat
     2161 ctcgataact cattaaagct tatcgatgat aagctgtcaa acatgagaat ttagcccgcc
     2221 taatgagcgg gctttttgtt gtgatccgcg ttgctggcgt ttttccatag gctccgcccc
     2281 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     2341 taaagatacc aggcgtttcc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     2401 gccgcttacc ggatacctgt ccgcctttct taattaactc tctagactca cctaggatcc
     2461 atgaattctg agatttttca aatcagtgcg caagacgtga cgtaagtatc cgagtcagtt
     2521 tttatttttc tactaatttg gtcgtttatt tcggcgtgta ggacatggca accgggcctg
     2581 aatttcgcgg gtattctgtt tctattccaa ctttttcttg atccgcagcc attaacgact
     2641 tttgaataga tacgctgaca cgccaagcct cgctagtcaa aagtgtacca aacaacgctt
     2701 tacagcaaga acggaatgcg cgtgacgctc gcggtgacgc catttcgcct tttcagaaat
     2761 ggataaatag ccttgcttcc tattatatct tcccaaatta ccaatacatt acactagcat
     2821 ctgaatttca taaccaatct cgatacacca aatcgaatcg atgagcccag aacgacgccc
     2881 ggccgacatc cgccgtgcca ccgaggcgga catgccggcg gtctgcacca tcgtcaacca
     2941 ctacatcgag acaagcacgg tcaacttccg taccgagccg caggaaccgc aggagtggac
     3001 ggacgacctc gtccgtctgc gggagcgcta tccctggctc gtcgccgagg tggacggcga
     3061 ggtcgccggc atcgcctacg cgggcccctg gaaggcacgc aacgcctacg actggacggc
     3121 cgagtcgacc gtgtacgtct ccccccgcca ccagcggacg ggactgggct ccacgctcta
     3181 cacccacctg ctgaagtccc tggaggcaca gggcttcaag agcgtggtcg ctgtcatcgg
     3241 gctgcccaac gacccgagcg tgcgcatgca cgaggcgctc ggatatgccc cccgcggcat
     3301 gctgcgggcg gccggcttca agcacgggaa ctggcatgac gtgggtttct ggcagctgga
     3361 cttcagcctg ccggtaccgc cccgtccggt cctgcccgtc accgagattt gactgcagaa
     3421 tcttggactc ccatgttggc aaaggcaacc aaacaaacaa tgaatgatcc gctcctgcat
     3481 atggggcggt ttgagtattt caactgccat ttgggctgaa ttgaagacat gctcctgtca
     3541 gaaattccgt gatcttactc aatattcagt aatctcggcc aatatcctaa atgtgcgtgg
     3601 ctttatctgt ctttgtattg tttcatcaat tcatgtaacg tttgcttttc ttatgaattt
     3661 tcaaataaat tatcagatcc gagctcgaat taattcggcg ttaattcagt acattaaaaa
     3721 cgtccgcaat gtgttattaa gttgtctaag cgtcaatttg tttacaccac aatatatcct
     3781 gccaccagcc agccaacagc tccccgaccg gcagctcggc acaaaatcac cactcgatac
     3841 aggcagccca tcagtccggg acggcgtcag cgggagagcc gttgtaaggc ggcagacttt
     3901 gctcatgtta ccgatgctat tcggaagaac ggcaactaag ctgccgggtt tgaaacacgg
     3961 atgatctcgc ggagggtagc atgttgattg taacgatgac agagcgttgc tgcctgtgat
     4021 caccgcggtt tcaaaatcgg ctccgtcgat actatgttat acgccaactt tgaaaacaac
     4081 tttgaaaaag ctgttttctg gtatttaagg ttttagaatg caaggaacag tgaattggag
     4141 ttcgtcttgt tataattagc ttcttggggt atctttaaat actgtagaaa agaggaagga
     4201 aataataaat ggctaaaatg agaatatcac cggaattgaa aaaactgatc gaaaaatacc
     4261 gctgcgtaaa agatacggaa ggaatgtctc ctgctaaggt atataagctg gtgggagaaa
     4321 atgaaaacct atatttaaaa atgacggaca gccggtataa agggaccacc tatgatgtgg
     4381 aacgggaaaa ggacatgatg ctatggctgg aaggaaagct gcctgttcca aaggtcctgc
     4441 actttgaacg gcatgatggc tggagcaatc tgctcatgag tgaggccgat ggcgtccttt
     4501 gctcggaaga gtatgaagat gaacaaagcc ctgaaaagat tatcgagctg tatgcggagt
     4561 gcatcaggct ctttcactcc atcgacatat cggattgtcc ctatacgaat agcttagaca
     4621 gccgcttagc cgaattggat tacttactga ataacgatct ggccgatgtg gattgcgaaa
     4681 actgggaaga agacactcca tttaaagatc cgcgcgagct gtatgatttt ttaaagacgg
     4741 aaaagcccga agaggaactt gtcttttccc acggcgacct gggagacagc aacatctttg
     4801 tgaaagatgg caaagtaagt ggctttattg atcttgggag aagcggcagg gcggacaagt
     4861 ggtatgacat tgccttctgc gtccggtcga tcagggagga tatcggggaa gaacagtatg
     4921 tcgagctatt ttttgactta ctggggatca agcctgattg ggagaaaata aaatattata
     4981 ttttactgga tgaattgttt tagtacctag aatgcatgac caaaatccct taacgtgagt
     5041 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     5101 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     5161 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     5221 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     5281 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     5341 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     5401 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     5461 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     5521 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     5581 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     5641 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     5701 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     5761 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     5821 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc
     5881 tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct
     5941 ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc
     6001 tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
     6061 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
     6121 gtcatcaccg aaacgcgcga ggcagggtgc cttgatgtgg gcgccggcgg tcgagtggcg
     6181 acggcgcggc ttgtccgcgc cctggtagat tgcctggccg taggccagcc atttttgagc
     6241 ggccagcggc cgcgataggc cgacgcgaag cggcggggcg tagggagcgc agcgaccgaa
     6301 gggtaggcgc tttttgcagc tcttcggctg tgcgctggcc agacagttat gcacaggcca
     6361 ggcgggtttt aagagtttta ataagtttta aagagtttta ggcggaaaaa tcgccttttt
     6421 tctcttttat atcagtcact tacatgtgtg accggttccc aatgtacggc tttgggttcc
     6481 caatgtacgg gttccggttc ccaatgtacg gctttgggtt cccaatgtac gtgctatcca
     6541 caggaaagag accttttcga cctttttccc ctgctagggc aatttgccct agcatctgct
     6601 ccgtacatta ggaaccggcg gatgcttcgc cctcgatcag gttgcggtag cgcatgacta
     6661 ggatcgggcc agcctgcccc gcctcctcct tcaaatcgta ctccggcagg tcatttgacc
     6721 cgatcagctt gcgcacggtg aaacagaact tcttgaactc tccggcgctg ccactgcgtt
     6781 cgtagatcgt cttgaacaac catctggctt ctgccttgcc tgcggcgcgg cgtgccaggc
     6841 ggtagagaaa acggccgatg ccgggatcga tcaaaaagta atcggggtga accgtcagca
     6901 cgtccgggtt cttgccttct gtgatctcgc ggtacatcca atcagctagc tcgatctcga
     6961 tgtactccgg ccgcccggtt tcgctcttta cgatcttgta gcggctaatc aaggcttcac
     7021 cctcggatac cgtcaccagg cggccgttct tggccttctt cgtacgctgc atggcaacgt
     7081 gcgtggtgtt taaccgaatg caggtttcta ccaggtcgtc tttctgcttt ccgccatcgg
     7141 ctcgccggca gaacttgagt acgtccgcaa cgtgtggacg gaacacgcgg ccgggcttgt
     7201 ctcccttccc ttcccggtat cggttcatgg attcggttag atgggaaacc gccatcagta
     7261 ccaggtcgta atcccacaca ctggccatgc cggccggccc tgcggaaacc tctacgtgcc
     7321 cgtctggaag ctcgtagcgg atcacctcgc cagctcgtcg gtcacgcttc gacagacgga
     7381 aaacggccac gtccatgatg ctgcgactat cgcgggtgcc cacgtcatag agcatcggaa
     7441 cgaaaaaatc tggttgctcg tcgcccttgg gcggcttcct aatcgacggc gcaccggctg
     7501 ccggcggttg ccgggattct ttgcggattc gatcagcggc cgcttgccac gattcaccgg
     7561 ggcgtgcttc tgcctcgatg cgttgccgct gggcggcctg cgcggccttc aacttctcca
     7621 ccaggtcatc acccagcgcc gcgccgattt gtaccgggcc ggatggtttg cgaccgctca
     7681 cgccgattcc tcgggcttgg gggttccagt gccattgcag ggccggcagg caacccagcc
     7741 gcttacgcct ggccaaccgc ccgttcctcc acacatgggg cattccacgg cgtcggtgcc
     7801 tggttgttct tgattttcca tgccgcctcc tttagccgct aaaattcatc tactcattta
     7861 ttcatttgct catttactct ggtagctgcg cgatgtattc agatagcagc tcggtaatgg
     7921 tcttgccttg gcgtaccgcg tacatcttca gcttggtgtg atcctccgcc ggcaactgaa
     7981 agttgacccg cttcatggct ggcgtgtctg ccaggctggc caacgttgca gccttgctgc
     8041 tgcgtgcgct cggacggccg gcacttagcg tgtttgtgct tttgctcatt ttctctttac
     8101 ctcattaact caaatgagtt ttgatttaat ttcagcggcc agcgcctgga cctcgcgggc
     8161 agcgtcgccc tcgggttctg attcaagaac ggttgtgccg gcggcggcag tgcctgggta
     8221 gctcacgcgc tgcgtgatac gggactcaag aatgggcagc tcgtacccgg ccagcgcctc
     8281 ggcaacctca ccgccgatgc gcgtgccttt gatcgcccgc gacacgacaa aggccgcttg
     8341 tagccttcca tccgtgacct caatgcgctg cttaaccagc tccaccaggt cggcggtggc
     8401 ccatatgtcg taagggcttg gctgcaccgg aatcagcacg aagtcggctg ccttgatcgc
     8461 ggacacagcc aagtccgccg cctggggcgc tccgtcgatc actacgaagt cgcgccggcc
     8521 gatggccttc acgtcgcggt caatcgtcgg gcggtcgatg ccgacaacgg ttagcggttg
     8581 atcttcccgc acggccgccc aatcgcgggc actgccctgg ggatcggaat cgactaacag
     8641 aacatcggcc ccggcgagtt gcagggcgcg ggctagatgg gttgcgatgg tcgtcttgcc
     8701 tgacccgcct ttctggttaa gtacagcgat aaccttcatg cgttcccctt gcgtatttgt
     8761 ttatttactc atcgcatcat atacgcagcg accgcatgac gcaagctgtt ttactcaaat
     8821 acacatcacc tttttagacg gcggcgctcg gtttcttcag cggccaagct ggccggccag
     8881 gccgccagct tggcatcaga caaaccggcc aggatttcat gcagccgcac ggttgagacg
     8941 tgcgcgggcg gctcgaacac gtacccggcc gcgatcatct ccgcctcgat ctcttcggta
     9001 atgaaaaacg gttcgtcctg gccgtcctgg tgcggtttca tgcttgttcc tcttggcgtt
     9061 cattctcggc ggccgccagg gcgtcggcct cggtcaatgc gtcctcacgg aaggcaccgc
     9121 gccgcctggc ctcggtgggc gtcacttcct cgctgcgctc aagtgcgcgg tacagggtcg
     9181 agcgatgcac gccaagcagt gcagccgcct ctttcacggt gcggccttcc tggtcgatca
     9241 gctcgcgggc gtgcgcgatc tgtgccgggg tgagggtagg gcgggggcca aacttcacgc
     9301 ctcgggcctt ggcggcctcg cgcccgctcc gggtgcggtc gatgattagg gaacgctcga
     9361 actcggcaat gccggcgaac acggtcaaca ccatgcggcc ggccggcgtg gtggtgtcgg
     9421 cccacggctc tgccaggcta cgcaggcccg cgccggcctc ctggatgcgc tcggcaatgt
     9481 ccagtaggtc gcgggtgctg cgggccaggc ggtctagcct ggtcactgtc acaacgtcgc
     9541 cagggcgtag gtggtcaagc atcctggcca gctccgggcg gtcgcgcctg gtgccggtga
     9601 tcttctcgga aaacagcttg gtgcagccgg ccgcgtgcag ttcggcccgt tggttggtca
     9661 agtcctggtc gtcggtgctg acgcgggcat agcccagcag gccagcggcg gcgctcttgt
     9721 tcatggcgta atgtctccgg ttctagtcgc aagtattcta ctttatgcga ctaaaacacg
     9781 cgacaagaaa acgccaggaa aagggcaggg cggcagcctg tcgcgtaact taggacttgt
     9841 gcgacatgtc gttttcagaa gacggctgca ctgaacgtca gaagccgact gcactatagc
     9901 agcggagggg ttggatcaaa gtactttgat cccgagggga accctgtggt tggcatgcac
     9961 atacaaatgg acgaacggat aaaccttttc acgccctttt aaatatccga ttattctaat
    10021 aaacgctctt ttctcttagg tttacccgcc aatatatcct gtcaaacact gatagtttaa
    10081 actgaaggcg ggaaacgaca atctgatcca agctcaagct gctctagcat tcgccattca
    10141 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg
    10201 cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac
    10261 gacgttgtaa aacgacggcc agtgccaagc ttgcatgcct gcaggtcctg ctgagcctcg
    10321 acatgttgtc gcaaaattcg ccctggaccc gcccaacgat ttgtcgtcac tgtcaaggtt
    10381 tgacctgcac ttcatttggg gcccacatac accaaaaaaa tgctgcataa ttctcggggc
    10441 agcaagtcgg ttacccggcc gccgtgctgg accgggttga atggtgcccg taactttcgg
    10501 tagagcggac ggccaatact caacttcaag gaatctcacc catgcgcgcc ggcggggaac
    10561 cggagttccc ttcagtgagc gttattagtt cgccgctcgg tgtgtcgtag atactagccc
    10621 ctggggcact tttgaaattt gaataagatt tatgtaatca gtcttttagg tttgaccggt
    10681 tctgccgctt tttttaaaat tggatttgta ataataaaac gcaattgttt gttattgtgg
    10741 cgctctatca tagatgtcgc tataaaccta ttcagcacaa tatattgttt tcattttaat
    10801 attgtacata taagtagtag ggtacaatca gtaaattgaa cggagaatat tattcataaa
    10861 aatacgatag taacgggtga tatattcatt agaatgaacc gaaaccggcg gtaaggatct
    10921 gagctacaca tgctcaggtt ttttacaacg tgcacaacag aattgaaagc aaatatcatg
    10981 cgatcatagg cgtctcgcat atctcattaa agcaggactc tagggactag tcccgggtct
    11041 taattaagtt tgcaagcagc agattacgcg cagaaaaaag ggatctcaag gagatccttt
    11101 ggatcacaaa aaaaaagtcc gctcattagg cgggttaaat tcccgggtac tgcagccgat
    11161 tcattaatgc aggttaacct ggcttttcga aattaatacg actcactata gggagaccgg
    11221 cagatctgat ccctactcca aaaatgtcaa agatacagtc tcagaagacc aaagggctat
    11281 tgagactttt caacaaaggg taatttcggg aaacctcctc ggattccatt gcccagctat
    11341 ctgtcacttc atcgaaagga cagtggaaaa ggaaggtggc tcctacaaat gccatcattg
    11401 cgataaagga aaggctatca ttcaagatgc ctctgccgac agtggtccca aagatggacc
    11461 cccacccacg aggagcatcg tgggaaaaga agacgttcca accacgtctt caaagcaagt
    11521 ggattgatgt gacatctcca ctgacgtaag ggatgacgca caatcccacc cctactccaa
    11581 aaatgtcaaa gatactgtct cagaagacca aagggctat
//
