LOCUS       sequence-167246-       11470 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  target  miRNA319/159  for destruction in Arabidopsis thaliana.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11470)
  AUTHORS   Tang G, Yan J, Gu Y, Qiao M, Fan R, Mao Y, Tang X
  TITLE     Construction of short tandem target mimic (STTM) to block the 
            functions of plant and animal microRNAs.
  JOURNAL   Methods. 2012 Oct;58(2):118-25. doi: 10.1016/j.ymeth.2012.10.006. 
            Epub 2012 Oct 23.
  PUBMED    23098881
REFERENCE   2  (bases 1 to 11470)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ymeth.2012.10.006"; journalName: "Methods"; date: 
            "2012-10"; volume: "58"; issue: "2"; pages: "118-25"
COMMENT     Sequence Label: pFGC5941-PacI-ath-STTM319/159
FEATURES             Location/Qualifiers
     source          1..11470
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..346
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     289..309
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     promoter        complement(1244..1262)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1245..1262)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     1901..1920
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(2023..2028,2029..2045,2046..2051))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     terminator      2313..3020
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     primer_bind     complement(3045..3062)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3045..3061)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3054..3076)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    3264..3288
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4589..5218
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5647..6720
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      6786..6980
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(7216..7238)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7324..7464
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7379..7398
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7479..7496)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7650..8238)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8325..9119)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    9544..9568
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(9654..9906)
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     CDS             complement(9916..10467)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(10473..10853)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     primer_bind     11045..11064
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        11045..11063
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tgagactttt caacaaaggg taatttcggg aaacctcctc ggattccatt gcccagctat
       61 ctgtcacctc atcgaaagga cagtagaaaa ggaaggtggc tcctacaaat gccatcattg
      121 cgataaagga aaggctatca ttcaagatgc ctctgccgac agtggtccca aagatggacc
      181 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
      241 ggattgatgt gacatctcca ctgacataag ggatgacgca caaccccact atccttcgca
      301 agacccttcc tctatataag gaagttcatt tcatttggag aggacagccc aagcttcaga
      361 gctccctcta tcaatccaaa gttgttgttg ttatggtcta atttaaatat ggtctaaaga
      421 agaagaatgg gagcacccct attcagtcca agaattcggt acgctgaaat caccagtctc
      481 tctctacaaa tctatctctc tctattttct ccataaataa tgtgtgagta gtttcccgat
      541 aagggaaatt agggttctta tagggtttcg ctcatgtgtt gagcatataa gaaaccctca
      601 gtatgtattt gtatttgtaa aatacttcta tcaataaaat ttctaattcc taaaaccaga
      661 atccagtact aaaatccaga tctcctaaag tccctataga tctttgtcgt gaatataaac
      721 cagacacgag acgactagac ctggagccca gacgccgttc gaagctagaa gtaccgctta
      781 ggcaggaggc cgttagggaa aagatgctaa ggcagggttg gttacgttga ctcccccgta
      841 ggtttggttt aaatatgatg aagtggacgg aaggaaggag gaagacaagg aaggataagg
      901 ttgcaggccc tgtgcaaggt aagaagatgg aaatttgata gaggtacgct actatactta
      961 tactatacgc taagggaatg attgtattta taccctatac cccctaataa ccccttatca
     1021 atttaagaaa taatccgcat aagcccccgc ttaaaaattg gtatcagagc catgaatagg
     1081 tctatgacca aaactcaaga ggataaaacc tcaccaaaat acgaaagagt ccttaactct
     1141 aaagataaaa gatctttcaa gatcaaaact agttccctca caccggtgac ggggatcgca
     1201 tgcgacctct agaccatcac attggagctt cagctgctcg agttctatag tgtcacctaa
     1261 atcgtatgtg tatgatacat aaggttatgt attaattgta gccgcgttct tacgtcgacg
     1321 taccaattac gccccgccct gtcactcatc gcagtactgt tgtaattcat taagcattct
     1381 gccgacatgg aagccatcac aaacggcatg atgaacctga atcgccagcg gcatcagcac
     1441 cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga agttgtccat
     1501 attggccacg tttaaatcaa aactggtgaa actcacccag ggattggctg agacgaaaaa
     1561 catattctca ataaaccctt tagggaaata ggccaggttt tcaccgtaac acgccacatc
     1621 ttgcgaatac atgtgtagaa actgccggaa atcgtcgtgg tattcactcc agagcgatga
     1681 aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg tgaacactat cccatattac
     1741 cagctcaccg tctttcattg ccatacggaa ctccggatga gcattcatca ggcgggcaag
     1801 aatgtgaata aaggccggat aaaacttgtg cttatttttc tttacggtct ttaaaaaggc
     1861 cgtaatatcc agctgaacag tctggttata ggtacattga gcaactgact gaaatgcctc
     1921 aaaatgttct ttacgatgcc attgggatat atcaacggtg gtatatccag tgatttcttt
     1981 ctccatttta gcttccttag ctcctgaaaa tctcgataac tcattaaagc ttatcgatga
     2041 taagctgtca aacatgagaa tttagcccgc ctaatgagcg ggctttttgt tgtgatccgc
     2101 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     2161 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc ccccctggaa
     2221 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     2281 ttaattaaga cccgggacta gtccctagag tcctgcttta atgagatatg cgagacgcct
     2341 atgatcgcat gatatttgct ttcaattctg ttgtgcacgt tgtaaaaaac ctgagcatgt
     2401 gtagctcaga tccttaccgc cggtttcggt tcattctaat gaatatatca cccgttacta
     2461 tcgtattttt atgaataata ttctccgttc aatttactga ttgtacccta ctacttatat
     2521 gtacaatatt aaaatgaaaa caatatattg tgctgaatag gtttatagcg acatctatga
     2581 tagagcgcca caataacaaa caattgcgtt ttattattac aaatccaatt ttaaaaaaag
     2641 cggcagaacc ggtcaaacct aaaagactga ttacataaat cttattcaaa tttcaaaagt
     2701 gccccagggg ctagtatcta cgacacaccg agcggcgaac taataacgct cactgaaggg
     2761 aactccggtt ccccgccggc gcgcatgggt gagattcctt gaagttgagt attggccgtc
     2821 cgctctaccg aaagttacgg gcaccattca acccggtcca gcacggcggc cgggtaaccg
     2881 acttgctgcc ccgagaatta tgcagcattt ttttggtgta tgtgggcccc aaatgaagtg
     2941 caggtcaaac cttgacagtg acgacaaatc gttgggcggg tccagggcga attttgcgac
     3001 aacatgtcga ggctcagcag gacctgcagg catgcaagct tggcactggc cgtcgtttta
     3061 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
     3121 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     3181 cgcagcctga atggcgaatg ctagagcagc ttgagcttgg atcagattgt cgtttcccgc
     3241 cttcagttta aactatcagt gtttgacagg atatattggc gggtaaacct aagagaaaag
     3301 agcgtttatt agaataatcg gatatttaaa agggcgtgaa aaggtttatc cgttcgtcca
     3361 tttgtatgtg catgccaacc acagggttcc cctcgggatc aaagtacttt gatccaaccc
     3421 ctccgctgct atagtgcagt cggcttctga cgttcagtgc agccgtcttc tgaaaacgac
     3481 atgtcgcaca agtcctaagt tacgcgacag gctgccgccc tgcccttttc ctggcgtttt
     3541 cttgtcgcgt gttttagtcg cataaagtag aatacttgcg actagaaccg gagacattac
     3601 gccatgaaca agagcgccgc cgctggcctg ctgggctatg cccgcgtcag caccgacgac
     3661 caggacttga ccaaccaacg ggccgaactg cacgcggccg gctgcaccaa gctgttttcc
     3721 gagaagatca ccggcaccag gcgcgaccgc ccggagctgg ccaggatgct tgaccaccta
     3781 cgccctggcg acgttgtgac agtgaccagg ctagaccgcc tggcccgcag cacccgcgac
     3841 ctactggaca ttgccgagcg catccaggag gccggcgcgg gcctgcgtag cctggcagag
     3901 ccgtgggccg acaccaccac gccggccggc cgcatggtgt tgaccgtgtt cgccggcatt
     3961 gccgagttcg agcgttccct aatcatcgac cgcacccgga gcgggcgcga ggccgccaag
     4021 gcccgaggcg tgaagtttgg cccccgccct accctcaccc cggcacagat cgcgcacgcc
     4081 cgcgagctga tcgaccagga aggccgcacc gtgaaagagg cggctgcact gcttggcgtg
     4141 catcgctcga ccctgtaccg cgcacttgag cgcagcgagg aagtgacgcc caccgaggcc
     4201 aggcggcgcg gtgccttccg tgaggacgca ttgaccgagg ccgacgccct ggcggccgcc
     4261 gagaatgaac gccaagagga acaagcatga aaccgcacca ggacggccag gacgaaccgt
     4321 ttttcattac cgaagagatc gaggcggaga tgatcgcggc cgggtacgtg ttcgagccgc
     4381 ccgcgcacgt ctcaaccgtg cggctgcatg aaatcctggc cggtttgtct gatgccaagc
     4441 tggcggcctg gccggccagc ttggccgctg aagaaaccga gcgccgccgt ctaaaaaggt
     4501 gatgtgtatt tgagtaaaac agcttgcgtc atgcggtcgc tgcgtatatg atgcgatgag
     4561 taaataaaca aatacgcaag gggaacgcat gaaggttatc gctgtactta accagaaagg
     4621 cgggtcaggc aagacgacca tcgcaaccca tctagcccgc gccctgcaac tcgccggggc
     4681 cgatgttctg ttagtcgatt ccgatcccca gggcagtgcc cgcgattggg cggccgtgcg
     4741 ggaagatcaa ccgctaaccg ttgtcggcat cgaccgcccg acgattgacc gcgacgtgaa
     4801 ggccatcggc cggcgcgact tcgtagtgat cgacggagcg ccccaggcgg cggacttggc
     4861 tgtgtccgcg atcaaggcag ccgacttcgt gctgattccg gtgcagccaa gcccttacga
     4921 catatgggcc accgccgacc tggtggagct ggttaagcag cgcattgagg tcacggatgg
     4981 aaggctacaa gcggcctttg tcgtgtcgcg ggcgatcaaa ggcacgcgca tcggcggtga
     5041 ggttgccgag gcgctggccg ggtacgagct gcccattctt gagtcccgta tcacgcagcg
     5101 cgtgagctac ccaggcactg ccgccgccgg cacaaccgtt cttgaatcag aacccgaggg
     5161 cgacgctgcc cgcgaggtcc aggcgctggc cgctgaaatt aaatcaaaac tcatttgagt
     5221 taatgaggta aagagaaaat gagcaaaagc acaaacacgc taagtgccgg ccgtccgagc
     5281 gcacgcagca gcaaggctgc aacgttggcc agcctggcag acacgccagc catgaagcgg
     5341 gtcaactttc agttgccggc ggaggatcac accaagctga agatgtacgc ggtacgccaa
     5401 ggcaagacca ttaccgagct gctatctgaa tacatcgcgc agctaccaga gtaaatgagc
     5461 aaatgaataa atgagtagat gaattttagc ggctaaagga ggcggcatgg aaaatcaaga
     5521 acaaccaggc accgacgccg tggaatgccc catgtgtgga ggaacgggcg gttggccagg
     5581 cgtaagcggc tgggttgcct gccggccctg caatggcact ggaaccccca agcccgagga
     5641 atcggcgtga gcggtcgcaa accatccggc ccggtacaaa tcggcgcggc gctgggtgat
     5701 gacctggtgg agaagttgaa ggccgcgcag gccgcccagc ggcaacgcat cgaggcagaa
     5761 gcacgccccg gtgaatcgtg gcaagcggcc gctgatcgaa tccgcaaaga atcccggcaa
     5821 ccgccggcag ccggtgcgcc gtcgattagg aagccgccca agggcgacga gcaaccagat
     5881 tttttcgttc cgatgctcta tgacgtgggc acccgcgata gtcgcagcat catggacgtg
     5941 gccgttttcc gtctgtcgaa gcgtgaccga cgagctggcg aggtgatccg ctacgagctt
     6001 ccagacgggc acgtagaggt ttccgcaggg ccggccggca tggccagtgt gtgggattac
     6061 gacctggtac tgatggcggt ttcccatcta accgaatcca tgaaccgata ccgggaaggg
     6121 aagggagaca agcccggccg cgtgttccgt ccacacgttg cggacgtact caagttctgc
     6181 cggcgagccg atggcggaaa gcagaaagac gacctggtag aaacctgcat tcggttaaac
     6241 accacgcacg ttgccatgca gcgtacgaag aaggccaaga acggccgcct ggtgacggta
     6301 tccgagggtg aagccttgat tagccgctac aagatcgtaa agagcgaaac cgggcggccg
     6361 gagtacatcg agatcgagct agctgattgg atgtaccgcg agatcacaga aggcaagaac
     6421 ccggacgtgc tgacggttca ccccgattac tttttgatcg atcccggcat cggccgtttt
     6481 ctctaccgcc tggcacgccg cgccgcaggc aaggcagaag ccagatggtt gttcaagacg
     6541 atctacgaac gcagtggcag cgccggagag ttcaagaagt tctgtttcac cgtgcgcaag
     6601 ctgatcgggt caaatgacct gccggagtac gatttgaagg aggaggcggg gcaggctggc
     6661 ccgatcctag tcatgcgcta ccgcaacctg atcgagggcg aagcatccgc cggttcctaa
     6721 tgtacggagc agatgctagg gcaaattgcc ctagcagggg aaaaaggtcg aaaaggtctc
     6781 tttcctgtgg atagcacgta cattgggaac ccaaagccgt acattgggaa ccggaacccg
     6841 tacattggga acccaaagcc gtacattggg aaccggtcac acatgtaagt gactgatata
     6901 aaagagaaaa aaggcgattt ttccgcctaa aactctttaa aacttattaa aactcttaaa
     6961 acccgcctgg cctgtgcata actgtctggc cagcgcacag ccgaagagct gcaaaaagcg
     7021 cctacccttc ggtcgctgcg ctccctacgc cccgccgctt cgcgtcggcc tatcgcggcc
     7081 gctggccgct caaaaatggc tggcctacgg ccaggcaatc taccagggcg cggacaagcc
     7141 gcgccgtcgc cactcgaccg ccggcgccca catcaaggca ccctgcctcg cgcgtttcgg
     7201 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
     7261 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
     7321 gggcgcagcc atgacccagt cacgtagcga tagcggagtg tatactggct taactatgcg
     7381 gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc
     7441 gtaaggagaa aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc
     7501 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     7561 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     7621 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     7681 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     7741 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     7801 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     7861 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     7921 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     7981 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     8041 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
     8101 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     8161 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     8221 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     8281 aacgaaaact cacgttaagg gattttggtc atgcattcta ggtactaaaa caattcatcc
     8341 agtaaaatat aatattttat tttctcccaa tcaggcttga tccccagtaa gtcaaaaaat
     8401 agctcgacat actgttcttc cccgatatcc tccctgatcg accggacgca gaaggcaatg
     8461 tcataccact tgtccgccct gccgcttctc ccaagatcaa taaagccact tactttgcca
     8521 tctttcacaa agatgttgct gtctcccagg tcgccgtggg aaaagacaag ttcctcttcg
     8581 ggcttttccg tctttaaaaa atcatacagc tcgcgcggat ctttaaatgg agtgtcttct
     8641 tcccagtttt cgcaatccac atcggccaga tcgttattca gtaagtaatc caattcggct
     8701 aagcggctgt ctaagctatt cgtataggga caatccgata tgtcgatgga gtgaaagagc
     8761 ctgatgcact ccgcatacag ctcgataatc ttttcagggc tttgttcatc ttcatactct
     8821 tccgagcaaa ggacgccatc ggcctcactc atgagcagat tgctccagcc atcatgccgt
     8881 tcaaagtgca ggacctttgg aacaggcagc tttccttcca gccatagcat catgtccttt
     8941 tcccgttcca catcataggt ggtcccttta taccggctgt ccgtcatttt taaatatagg
     9001 ttttcatttt ctcccaccag cttatatacc ttagcaggag acattccttc cgtatctttt
     9061 acgcagcggt atttttcgat cagttttttc aattccggtg atattctcat tttagccatt
     9121 tattatttcc ttcctctttt ctacagtatt taaagatacc ccaagaagct aattataaca
     9181 agacgaactc caattcactg ttccttgcat tctaaaacct taaataccag aaaacagctt
     9241 tttcaaagtt gttttcaaag ttggcgtata acatagtatc gacggagccg attttgaaac
     9301 cgcggtgatc acaggcagca acgctctgtc atcgttacaa tcaacatgct accctccgcg
     9361 agatcatccg tgtttcaaac ccggcagctt agttgccgtt cttccgaata gcatcggtaa
     9421 catgagcaaa gtctgccgcc ttacaacggc tctcccgctg acgccgtccc ggactgatgg
     9481 gctgcctgta tcgagtggtg attttgtgcc gagctgccgg tcggggagct gttggctggc
     9541 tggtggcagg atatattgtg gtgtaaacaa attgacgctt agacaactta ataacacatt
     9601 gcggacgttt ttaatgtact gaattaacgc cgaattaatt cgagctcgga tctgataatt
     9661 tatttgaaaa ttcataagaa aagcaaacgt tacatgaatt gatgaaacaa tacaaagaca
     9721 gataaagcca cgcacattta ggatattggc cgagattact gaatattgag taagatcacg
     9781 gaatttctga caggagcatg tcttcaattc agcccaaatg gcagttgaaa tactcaaacc
     9841 gccccatatg caggagcgga tcattcattg tttgtttggt tgcctttgcc aacatgggag
     9901 tccaagattc tgcagtcaaa tctcggtgac gggcaggacc ggacggggcg gtaccggcag
     9961 gctgaagtcc agctgccaga aacccacgtc atgccagttc ccgtgcttga agccggccgc
    10021 ccgcagcatg ccgcgggggg catatccgag cgcctcgtgc atgcgcacgc tcgggtcgtt
    10081 gggcagcccg atgacagcga ccacgctctt gaagccctgt gcctccaggg acttcagcag
    10141 gtgggtgtag agcgtggagc ccagtcccgt ccgctggtgg cggggggaga cgtacacggt
    10201 cgactcggcc gtccagtcgt aggcgttgcg tgccttccag gggcccgcgt aggcgatgcc
    10261 ggcgacctcg ccgtccacct cggcgacgag ccagggatag cgctcccgca gacggacgag
    10321 gtcgtccgtc cactcctgcg gttcctgcgg ctcggtacgg aagttgaccg tgcttgtctc
    10381 gatgtagtgg ttgacgatgg tgcagaccgc cggcatgtcc gcctcggtgg cacggcggat
    10441 gtcggccggg cgtcgttctg ggctcatcga ttcgatttgg tgtatcgaga ttggttatga
    10501 aattcagatg ctagtgtaat gtattggtaa tttgggaaga tataatagga agcaaggcta
    10561 tttatccatt tctgaaaagg cgaaatggcg tcaccgcgag cgtcacgcgc attccgttct
    10621 tgctgtaaag cgttgtttgg tacacttttg actagcgagg cttggcgtgt cagcgtatct
    10681 attcaaaagt cgttaatggc tgcggatcaa gaaaaagttg gaatagaaac agaatacccg
    10741 cgaaattcag gcccggttgc catgtcctac acgccgaaat aaacgaccaa attagtagaa
    10801 aaataaaaac tgactcggat acttacgtca cgtcttgcgc actgatttga aaaatctcag
    10861 aattcatgga tcctaggtga gtctagagag ttaattaagt ttgcaagcag cagattacgc
    10921 gcagaaaaaa aggatctcaa ggagatcctt tggatcacaa aaaaaaagcc cgctcattag
    10981 gcgggttaaa ttcccgggta ctgcagccga ttcattaatg caggttaacc tggcttatcg
    11041 aaattaatac gactcactat agggagaccg gcagatctga tccctactcc aaaaatgtca
    11101 aagatacagt ctcagaagac caaagggcta ttgagacttt tcaacaaagg gtaatttcgg
    11161 gaaacctcct cggattccat tgcccagcta tctgtcactt catcgaaagg acagtggaaa
    11221 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggctatc attcaagatg
    11281 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtgggaaaag
    11341 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgacatctcc actgacgtaa
    11401 gggatgacgc acaatcccac ccctactcca aaaatgtcaa agatactgtc tcagaagacc
    11461 aaagggctat
//
