LOCUS       sequence-167248-       13242 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  target  miRNA390  for destruction in Arabidopsis thaliana.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13242)
  AUTHORS   Tang G, Yan J, Gu Y, Qiao M, Fan R, Mao Y, Tang X
  TITLE     Construction of short tandem target mimic (STTM) to block the 
            functions of plant and animal microRNAs.
  JOURNAL   Methods. 2012 Oct;58(2):118-25. doi: 10.1016/j.ymeth.2012.10.006. 
            Epub 2012 Oct 23.
  PUBMED    23098881
REFERENCE   2  (bases 1 to 13242)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ymeth.2012.10.006"; journalName: "Methods"; date: 
            "2012-10"; volume: "58"; issue: "2"; pages: "118-25"
COMMENT     Sequence Label: pFGC5941DR5-PacI-ath-STTM390
FEATURES             Location/Qualifiers
     source          1..13242
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     360..379
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        360..378
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        786..1131
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    1292..1468
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(2030..2048)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2031..2048)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     2687..2706
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(2809..2814,2815..2831,2832..2837))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     promoter        3112..3492
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     CDS             3498..4049
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     terminator      4059..4311
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     misc_feature    4397..4421
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             4846..5640
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      5727..6315
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6469..6486
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6501..6641
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(6567..6586)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6727..6749
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      6985..7179
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(7245..8318)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(8747..9376)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    10677..10701
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     10889..10911
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10903..10920
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10904..10920
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        11009..11055
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     CDS             join(11074..11088,11279..13096)
                     /codon_start=1
                     /product="beta-glucuronidase"
                     /label=GUS
                     /note="This version of the gusA gene has a 5' extension 
                     with a catalase intron to ensure expression in plants but 
                     not bacteria."
                     /translation="MVDLRNRRLVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWES
                     ALQESRAIAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGK
                     VWVNNQEVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGK
                     KKQSYFHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSV
                     ELRDADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRS
                     VAVKGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYP
                     YAEEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQA
                     IKELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDT
                     LAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKK
                     GIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     intron          11089..11278
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
     CDS             13103..13120
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      join(13155..13242,1..165)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
ORIGIN
        1 acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga tgggttttta
       61 tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata tagcgcgcaa
      121 actaggataa attatcgcgc gcggtgtcat ctatgttact agatcgggaa ttaaactatc
      181 agtgtttgac aggatatatt ggcggttaat taagtttgca agcagcagat tacgcgcaga
      241 aaaaaaggat ctcaaggaga tcctttggat cacaaaaaaa aagcccgctc attaggcggg
      301 ttaaattccc gggtactgca gccgattcat taatgcaggt taacctggct tatcgaaatt
      361 aatacgactc actataggga gaccggcaga tctgatccct actccaaaaa tgtcaaagat
      421 acagtctcag aagatcaaag ggctattgag acttttcaac aaagggtaat ttcgggaaac
      481 ctcctcggat tccattgccc agctatctgt cacttcatcg aaaggacagt ggaaaaggaa
      541 ggtggctcct acaaatgcca tcattgcgat aaaggaaagg ctatcattca agatgcctct
      601 gccgacagtg gtcccaaaga tggaccccca cccacgagga gcatcgtggg aaaagaagac
      661 gttccaacca cgtcttcaaa gcaagtggat tgatgtgaca tctccactga cgtaagggat
      721 gacgcacaat cccaccccta ctccaaaaat gtcaaagata ctgtctcaga agaccaaagg
      781 gctattgaga cttttcaaca aagggtaatt tcgggaaacc tcctcggatt ccattgccca
      841 gctatctgtc acctcatcga aaggacagta gaaaaggaag gtggctccta caaatgccat
      901 cattgcgata aaggaaaggc tatcattcaa gatgcctctg ccgacagtgg tcccaaagat
      961 ggacccccac ccacgaggag catcgtggaa aaagaagacg ttccaaccac gtcttcaaag
     1021 caagtggatt gatgtgacat ctccactgac ataagggatg acgcacaacc ccactatcct
     1081 tcgcaagacc cttcctctat ataaggaagt tcatttcatt tggagaggac agcccaagct
     1141 tggcgctatc ccctatcctg agcttgttgt tgttgttatg gtctaattta aatatggtct
     1201 aaagaagaag aatggcgcta tcccctatcc tgagcttgaa ttcggtacgc tgaaatcacc
     1261 agtctctctc tacaaatcta tctctctcta ttttctccat aaataatgtg tgagtagttt
     1321 cccgataagg gaaattaggg ttcttatagg gtttcgctca tgtgttgagc atataagaaa
     1381 cccttagtat gtatttgtat ttgtaaaata cttctatcaa taaaatttct aattcctaaa
     1441 accaaaatcc agtactaaaa tccagatctc ctaaagtccc tatagatctt tgtcgtgaat
     1501 ataaaccaga cacgagacga ctagacctgg agcccagacg ccgttcgaag ctagaagtac
     1561 cgcttaggca ggaggccgtt agggaaaaga tgctaaggca gggttggtta cgttgactcc
     1621 cccgtaggtt tggtttaaat atgatgaagt ggacggaagg aaggaggaag acaaggaagg
     1681 ataaggttgc aggccctgtg caaggtaaga agatggaaat ttgatagagg tacgctacta
     1741 tacttatact atacgctaag ggaatgattg tatttatacc ctataccccc taataacccc
     1801 ttatcaattt aagaaataat ccgcataagc ccccgcttaa aaattggtat cagagccatg
     1861 aataggtcta tgaccaaaac tcaagaggat aaaacctcac caaaatacga aagagtcctt
     1921 aactctaaag ataaaagatc tttcaaaatc aaaactagtt ccctcacacc ggtgacgggg
     1981 atcgcatgcg acctctagac catcacattg gagcttcagc tgctcgagtt ctatagtgtc
     2041 acctaaatcg tatgtgtatg atacataagg ttatgtatta attgtagccg cgttcttacg
     2101 tcgacgcacc aattacgccc cgccctgtca ctcatcgcag tactgttgta attcattaag
     2161 cattctgccg acatggaagc catcacaaac ggcatgatga acctgaatcg ccagcggcat
     2221 cagcaccttg tcgccttgcg tataatattt gcccatggtg aaaacggggg cgaagaagtt
     2281 gtccatattg gccacgttta aatcaaaact ggtgaaactc acccagggat tggctgagac
     2341 gaaaaacata ttctcaataa accctttagg gaaataggcc aggttttcac cgtaacacgc
     2401 cacatcttgc gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt cactccagag
     2461 cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa caagggtgaa cactatccca
     2521 tattaccagc tcaccgtctt tcattgccat acggaactcc ggatgagcat tcatcaggcg
     2581 ggcaagaatg tgaataaagg ccggataaaa cttgtgctta tttttcttta cggtctttaa
     2641 aaaggccgta atatccagct gaacggtctg gttataggta cattgagcaa ctgactgaaa
     2701 tgcctcaaaa tgttctttac gatgccattg ggatatatca acggtggtat atccagtgat
     2761 ttctttctcc attttagctt ccttagctcc tgaaaatctc gataactcat taaagcttat
     2821 cgatgataag ctgtcaaaca tgagaattta gcccgcctaa tgagcgggct ttttgttgtg
     2881 atccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     2941 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     3001 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     3061 cctttcttaa ttaactctct agactcacct aggatccatg aattctgaga tttttcaaat
     3121 cagtgcgcaa gacgtgacgt aagtatccga gtcagttttt atttttctac taatttggtc
     3181 gtttatttcg gcgtgtagga catggcaacc gggcctgaat ttcgcgggta ttctgtttct
     3241 attccaactt tttcttgatc cgcagccatt aacgactttt gaatagatac gctgacacgc
     3301 caagcctcgc tagtcaaaag tgtaccaaac aacgctttac agcaagaacg gaatgcgcgt
     3361 gacgctcgcg gtgacgccat ttcgcctttt cagaaatgga taaatagcct tgcttcctat
     3421 tatatcttcc caaattacca atacattaca ctagcatctg aatttcataa ccaatctcga
     3481 tacaccaaat cgaatcgatg agcccagaac gacgcccggc cgacatccgc cgtgccaccg
     3541 aggcggacat gccggcggtc tgcaccatcg tcaaccacta catcgagaca agcacggtca
     3601 acttccgtac cgagccgcag gaaccgcagg agtggacgga cgacctcgtc cgtctgcggg
     3661 agcgctatcc ctggctcgtc gccgaggtgg acggcgaggt cgccggcatc gcctacgcgg
     3721 gcccctggaa ggcacgcaac gcctacgact ggacggccga gtcgaccgtg tacgtctccc
     3781 cccgccacca gcggacggga ctgggctcca cgctctacac ccacctgctg aagtccctgg
     3841 aggcacaggg cttcaagagc gtggtcgctg tcatcgggct gcccaacgac ccgagcgtgc
     3901 gcatgcacga ggcgctcgga tatgcccccc gcggcatgct gcgggcggcc ggcttcaagc
     3961 acgggaactg gcatgacgtg ggtttctggc agctggactt cagcctgccg gtaccgcccc
     4021 gtccggtcct gcccgtcacc gagatttgac tgcagaatct tggactccca tgttggcaaa
     4081 ggcaaccaaa caaacaatga atgatccgct cctgcatatg gggcggtttg agtatttcaa
     4141 ctgccatttg ggctgaattg aagacatgct cctgtcagaa attccgtgat cttactcaat
     4201 attcagtaat ctcggccaat atcctaaatg tgcgtggctt tatctgtctt tgtattgttt
     4261 catcaattca tgtaacgttt gcttttctta tgaattttca aataaattat cagatccgag
     4321 ctcgaattaa ttcggcgtta attcagtaca ttaaaaacgt ccgcaatgtg ttattaagtt
     4381 gtctaagcgt caatttgttt acaccacaat atatcctgcc accagccagc caacagctcc
     4441 ccgaccggca gctcggcaca aaatcaccac tcgatacagg cagcccatca gtccgggacg
     4501 gcgtcagcgg gagagccgtt gtaaggcggc agactttgct catgttaccg atgctattcg
     4561 gaagaacggc aactaagctg ccgggtttga aacacggatg atctcgcgga gggtagcatg
     4621 ttgattgtaa cgatgacaga gcgttgctgc ctgtgatcac cgcggtttca aaatcggctc
     4681 cgtcgatact atgttatacg ccaactttga aaacaacttt gaaaaagctg ttttctggta
     4741 tttaaggttt tagaatgcaa ggaacagtga attggagttc gtcttgttat aattagcttc
     4801 ttggggtatc tttaaatact gtagaaaaga ggaaggaaat aataaatggc taaaatgaga
     4861 atatcaccgg aattgaaaaa actgatcgaa aaataccgct gcgtaaaaga tacggaagga
     4921 atgtctcctg ctaaggtata taagctggtg ggagaaaatg aaaacctata tttaaaaatg
     4981 acggacagcc ggtataaagg gaccacctat gatgtggaac gggaaaagga catgatgcta
     5041 tggctggaag gaaagctgcc tgttccaaag gtcctgcact ttgaacggca tgatggctgg
     5101 agcaatctgc tcatgagtga ggccgatggc gtcctttgct cggaagagta tgaagatgaa
     5161 caaagccctg aaaagattat cgagctgtat gcggagtgca tcaggctctt tcactccatc
     5221 gacatatcgg attgtcccta tacgaatagc ttagacagcc gcttagccga attggattac
     5281 ttactgaata acgatctggc cgatgtggat tgcgaaaact gggaagaaga cactccattt
     5341 aaagatccgc gcgagctgta tgatttttta aagacggaaa agcccgaaga ggaacttgtc
     5401 ttttcccacg gcgacctggg agacagcaac atctttgtga aagatggcaa agtaagtggc
     5461 tttattgatc ttgggagaag cggcagggcg gacaagtggt atgacattgc cttctgcgtc
     5521 cggtcgatca gggaggatat cggggaagaa cagtatgtcg agctattttt tgacttactg
     5581 gggatcaagc ctgattggga gaaaataaaa tattatattt tactggatga attgttttag
     5641 tacctagaat gcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     5701 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     5761 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     5821 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
     5881 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     5941 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     6001 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     6061 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     6121 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     6181 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     6241 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     6301 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     6361 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     6421 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     6481 agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
     6541 tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     6601 tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac
     6661 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
     6721 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc
     6781 agggtgcctt gatgtgggcg ccggcggtcg agtggcgacg gcgcggcttg tccgcgccct
     6841 ggtagattgc ctggccgtag gccagccatt tttgagcggc cagcggccgc gataggccga
     6901 cgcgaagcgg cggggcgtag ggagcgcagc gaccgaaggg taggcgcttt ttgcagctct
     6961 tcggctgtgc gctggccaga cagttatgca caggccaggc gggttttaag agttttaata
     7021 agttttaaag agttttaggc ggaaaaatcg ccttttttct cttttatatc agtcacttac
     7081 atgtgtgacc ggttcccaat gtacggcttt gggttcccaa tgtacgggtt ccggttccca
     7141 atgtacggct ttgggttccc aatgtacgtg ctatccacag gaaagagacc ttttcgacct
     7201 ttttcccctg ctagggcaat ttgccctagc atctgctccg tacattagga accggcggat
     7261 gcttcgccct cgatcaggtt gcggtagcgc atgactagga tcgggccagc ctgccccgcc
     7321 tcctccttca aatcgtactc cggcaggtca tttgacccga tcagcttgcg cacggtgaaa
     7381 cagaacttct tgaactctcc ggcgctgcca ctgcgttcgt agatcgtctt gaacaaccat
     7441 ctggcttctg ccttgcctgc ggcgcggcgt gccaggcggt agagaaaacg gccgatgccg
     7501 ggatcgatca aaaagtaatc ggggtgaacc gtcagcacgt ccgggttctt gccttctgtg
     7561 atctcgcggt acatccaatc agctagctcg atctcgatgt actccggccg cccggtttcg
     7621 ctctttacga tcttgtagcg gctaatcaag gcttcaccct cggataccgt caccaggcgg
     7681 ccgttcttgg ccttcttcgt acgctgcatg gcaacgtgcg tggtgtttaa ccgaatgcag
     7741 gtttctacca ggtcgtcttt ctgctttccg ccatcggctc gccggcagaa cttgagtacg
     7801 tccgcaacgt gtggacggaa cacgcggccg ggcttgtctc ccttcccttc ccggtatcgg
     7861 ttcatggatt cggttagatg ggaaaccgcc atcagtacca ggtcgtaatc ccacacactg
     7921 gccatgccgg ccggccctgc ggaaacctct acgtgcccgt ctggaagctc gtagcggatc
     7981 acctcgccag ctcgtcggtc acgcttcgac agacggaaaa cggccacgtc catgatgctg
     8041 cgactatcgc gggtgcccac gtcatagagc atcggaacga aaaaatctgg ttgctcgtcg
     8101 cccttgggcg gcttcctaat cgacggcgca ccggctgccg gcggttgccg ggattctttg
     8161 cggattcgat cagcggccgc ttgccacgat tcaccggggc gtgcttctgc ctcgatgcgt
     8221 tgccgctggg cggcctgcgc ggccttcaac ttctccacca ggtcatcacc cagcgccgcg
     8281 ccgatttgta ccgggccgga tggtttgcga ccgctcacgc cgattcctcg ggcttggggg
     8341 ttccagtgcc attgcagggc cggcaggcaa cccagccgct tacgcctggc caaccgcccg
     8401 ttcctccaca catggggcat tccacggcgt cggtgcctgg ttgttcttga ttttccatgc
     8461 cgcctccttt agccgctaaa attcatctac tcatttattc atttgctcat ttactctggt
     8521 agctgcgcga tgtattcaga tagcagctcg gtaatggtct tgccttggcg taccgcgtac
     8581 atcttcagct tggtgtgatc ctccgccggc aactgaaagt tgacccgctt catggctggc
     8641 gtgtctgcca ggctggccaa cgttgcagcc ttgctgctgc gtgcgctcgg acggccggca
     8701 cttagcgtgt ttgtgctttt gctcattttc tctttacctc attaactcaa atgagttttg
     8761 atttaatttc agcggccagc gcctggacct cgcgggcagc gtcgccctcg ggttctgatt
     8821 caagaacggt tgtgccggcg gcggcagtgc ctgggtagct cacgcgctgc gtgatacggg
     8881 actcaagaat gggcagctcg tacccggcca gcgcctcggc aacctcaccg ccgatgcgcg
     8941 tgcctttgat cgcccgcgac acgacaaagg ccgcttgtag ccttccatcc gtgacctcaa
     9001 tgcgctgctt aaccagctcc accaggtcgg cggtggccca tatgtcgtaa gggcttggct
     9061 gcaccggaat cagcacgaag tcggctgcct tgatcgcgga cacagccaag tccgccgcct
     9121 ggggcgctcc gtcgatcact acgaagtcgc gccggccgat ggccttcacg tcgcggtcaa
     9181 tcgtcgggcg gtcgatgccg acaacggtta gcggttgatc ttcccgcacg gccgcccaat
     9241 cgcgggcact gccctgggga tcggaatcga ctaacagaac atcggccccg gcgagttgca
     9301 gggcgcgggc tagatgggtt gcgatggtcg tcttgcctga cccgcctttc tggttaagta
     9361 cagcgataac cttcatgcgt tccccttgcg tatttgttta tttactcatc gcatcatata
     9421 cgcagcgacc gcatgacgca agctgtttta ctcaaataca catcaccttt ttagacggcg
     9481 gcgctcggtt tcttcagcgg ccaagctggc cggccaggcc gccagcttgg catcagacaa
     9541 accggccagg atttcatgca gccgcacggt tgagacgtgc gcgggcggct cgaacacgta
     9601 cccggccgcg atcatctccg cctcgatctc ttcggtaatg aaaaacggtt cgtcctggcc
     9661 gtcctggtgc ggtttcatgc ttgttcctct tggcgttcat tctcggcggc cgccagggcg
     9721 tcggcctcgg tcaatgcgtc ctcacggaag gcaccgcgcc gcctggcctc ggtgggcgtc
     9781 acttcctcgc tgcgctcaag tgcgcggtac agggtcgagc gatgcacgcc aagcagtgca
     9841 gccgcctctt tcacggtgcg gccttcctgg tcgatcagct cgcgggcgtg cgcgatctgt
     9901 gccggggtga gggtagggcg ggggccaaac ttcacgcctc gggccttggc ggcctcgcgc
     9961 ccgctccggg tgcggtcgat gattagggaa cgctcgaact cggcaatgcc ggcgaacacg
    10021 gtcaacacca tgcggccggc cggcgtggtg gtgtcggccc acggctctgc caggctacgc
    10081 aggcccgcgc cggcctcctg gatgcgctcg gcaatgtcca gtaggtcgcg ggtgctgcgg
    10141 gccaggcggt ctagcctggt cactgtcaca acgtcgccag ggcgtaggtg gtcaagcatc
    10201 ctggccagct ccgggcggtc gcgcctggtg ccggtgatct tctcggaaaa cagcttggtg
    10261 cagccggccg cgtgcagttc ggcccgttgg ttggtcaagt cctggtcgtc ggtgctgacg
    10321 cgggcatagc ccagcaggcc agcggcggcg ctcttgttca tggcgtaatg tctccggttc
    10381 tagtcgcaag tattctactt tatgcgacta aaacacgcga caagaaaacg ccaggaaaag
    10441 ggcagggcgg cagcctgtcg cgtaacttag gacttgtgcg acatgtcgtt ttcagaagac
    10501 ggctgcactg aacgtcagaa gccgactgca ctatagcagc ggaggggttg gatcaaagta
    10561 ctttgatccc gaggggaacc ctgtggttgg catgcacata caaatggacg aacggataaa
    10621 ccttttcacg cccttttaaa tatccgatta ttctaataaa cgctcttttc tcttaggttt
    10681 acccgccaat atatcctgtc aaacactgat agtttaaact gaaggcggga aacgacaatc
    10741 tgatccaagc tcaagctgct ctagcattcg ccattcaggc tgcgcaactg ttgggaaggg
    10801 cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg
    10861 cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt
    10921 gccaagcttc cttttgtctc ccttttgtct cccttttgtc tcccttttgt ctcccttttg
    10981 tctccctttt gtctcccttt tgtctctcgc aagacccttc ctctatataa ggaagttcat
    11041 ttcatttgga gagaacacgg gggactcttg accatggtag atctgagggt aaatttctag
    11101 tttttctcct tcattttctt ggttaggacc cttttctctt tttatttttt tgagctttga
    11161 tctttcttta aactgatcta ttttttaatt gattggttat ggtgtaaata ttacatagct
    11221 ttaactgata atctgattac tttatttcgt gtgtctatga tgatgatgat agttacagaa
    11281 ccgacgactc gtccgtcctg tagaaacccc aacccgtgaa atcaaaaaac tcgacggcct
    11341 gtgggcattc agtctggatc gcgaaaactg tggaattgat cagcgttggt gggaaagcgc
    11401 gttacaagaa agccgggcaa ttgctgtgcc aggcagtttt aacgatcagt tcgccgatgc
    11461 agatattcgt aattatgcgg gcaacgtctg gtatcagcgc gaagtcttta taccgaaagg
    11521 ttgggcaggc cagcgtatcg tgctgcgttt cgatgcggtc actcattacg gcaaagtgtg
    11581 ggtcaataat caggaagtga tggagcatca gggcggctat acgccatttg aagccgatgt
    11641 cacgccgtat gttattgccg ggaaaagtgt acgtatcacc gtttgtgtga acaacgaact
    11701 gaactggcag actatcccgc cgggaatggt gattaccgac gaaaacggca agaaaaagca
    11761 gtcttacttc catgatttct ttaactatgc cggaatccat cgcagcgtaa tgctctacac
    11821 cacgccgaac acctgggtgg acgatatcac cgtggtgacg catgtcgcgc aagactgtaa
    11881 ccacgcgtct gttgactggc aggtggtggc caatggtgat gtcagcgttg aactgcgtga
    11941 tgcggatcaa caggtggttg caactggaca aggcactagc gggactttgc aagtggtgaa
    12001 tccgcacctc tggcaaccgg gtgaaggtta tctctatgaa ctgtgcgtca cagccaaaag
    12061 ccagacagag tgtgatatct acccgcttcg cgtcggcatc cggtcagtgg cagtgaaggg
    12121 cgaacagttc ctgattaacc acaaaccgtt ctactttact ggctttggtc gtcatgaaga
    12181 tgcggacttg cgtggcaaag gattcgataa cgtgctgatg gtgcacgacc acgcattaat
    12241 ggactggatt ggggccaact cctaccgtac ctcgcattac ccttacgctg aagagatgct
    12301 cgactgggca gatgaacatg gcatcgtggt gattgatgaa actgctgctg tcggctttaa
    12361 cctctcttta ggcattggtt tcgaagcggg caacaagccg aaagaactgt acagcgaaga
    12421 ggcagtcaac ggggaaactc agcaagcgca cttacaggcg attaaagagc tgatagcgcg
    12481 tgacaaaaac cacccaagcg tggtgatgtg gagtattgcc aacgaaccgg atacccgtcc
    12541 gcaaggtgca cgggaatatt tcgcgccact ggcggaagca acgcgtaaac tcgacccgac
    12601 gcgtccgatc acctgcgtca atgtaatgtt ctgcgacgct cacaccgata ccatcagcga
    12661 tctctttgat gtgctgtgcc tgaaccgtta ttacggatgg tatgtccaaa gcggcgattt
    12721 ggaaacggca gagaaggtac tggaaaaaga acttctggcc tggcaggaga aactgcatca
    12781 gccgattatc atcaccgaat acggcgtgga tacgttagcc gggctgcact caatgtacac
    12841 cgacatgtgg agtgaagagt atcagtgtgc atggctggat atgtatcacc gcgtctttga
    12901 tcgcgtcagc gccgtcgtcg gtgaacaggt atggaatttc gccgattttg cgacctcgca
    12961 aggcatattg cgcgttggcg gtaacaagaa agggatcttc actcgcgacc gcaaaccgaa
    13021 gtcggcggct tttctgctgc aaaaacgctg gactggcatg aacttcggtg aaaaaccgca
    13081 gcagggaggc aaacaagcta gccaccacca ccaccaccac gtgtgaatta caggtgacca
    13141 gctcgaattt ccccgatcgt tcaaacattt ggcaataaag tttcttaaga ttgaatcctg
    13201 ttgccggtct tgcgatgatt atcatataat ttctgttgaa tt
//
