LOCUS       sequence-167253-        1063 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For Flpe/FRT-based Supernova, pK036 should be used with pK068 etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Luo W, Mizuno H, Iwata R, Nakazawa S, Yasuda K, Itohara S, Iwasato T
  TITLE     Supernova: A Versatile Vector System for Single-Cell Labeling and 
            Gene Function Studies in vivo.
  JOURNAL   Sci Rep. 2016 Oct 24;6:35747. doi: 10.1038/srep35747.
  PUBMED    27775045
REFERENCE   2  (bases 1 to 1063)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-10-24"; volume: "6:35747. doi"; pages: " 
            10.1038/srep35747"
COMMENT     Sequence Label: pK036.TRE-Flpe-WPRE (Supernova)
FEATURES             Location/Qualifiers
     source          1..1063
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     93..111
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        128..442
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    136..154
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    172..190
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    207..225
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    243..261
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    279..297
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    314..332
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    350..368
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     415..439
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      512..521
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             524..544
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag
       61 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
      121 ttcactcgag tttactccct atcagtgata gagaacgtat gtcgagttta ctccctatca
      181 gtgatagaga acgatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt
      241 actccctatc agtgatagag aacgtatgtc gagtttactc cctatcagtg atagagaacg
      301 tatgtcgagt ttatccctat cagtgataga gaacgtatgt cgagtttact ccctatcagt
      361 gatagagaac gtatgtcgag gtaggcgtgt acggtgggag gcctatataa gcagagctcg
      421 tttagtgaac cgtcagatcg cctggagaat tcgagctcgg tacccgggga tccttgtgct
      481 gtctcatcat tttggcaaag aattacccgc cgccaccatg gctcccaaga agaagaggaa
      541 ggtgatgagt caatttgata tattatgtaa aacaccacct aaggtcctgg ttcgtcagtt
      601 tgtggaaagg tttgaaagac cttcagggga aaaaatagca tcatgtgctg ctgaactaac
      661 ctatttatgt tggatgatta ctcataacgg aacagcaatc aagagagcca cattcatgag
      721 ctataatact atcataagca attcgctgag tttcgatatt gtcaacaaat cactccagtt
      781 taaatacaag acgcaaaaag caacaattct ggaagcctca ttaaagaaat taattcctgc
      841 ttgggaattt acaattattc cttacaatgg acaaaaacat caatctgata tcactgatat
      901 tgtaagtagt ttgcaattac agttcgaatc atcggaagaa gcagataagg gaaatagcca
      961 cagtaaaaaa atgcttaaag cacttctaag tgagggtgaa agcatctggg agatcactga
     1021 gaaaatacta aattcgtttg agtatacctc gagatttaca aaa
//
