LOCUS       sequence-167254-        4365 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing CDK17 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4365)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4365)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_CDK17_p.R177H
FEATURES             Location/Qualifiers
     source          1..4365
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2244..2343)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2360..2379)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2361..2379)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2384..2400)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2384..2400)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2831..3622
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3715..4303
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4204..4223
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcacc atgaaaaaat ttaagagaag gctatccctc acactccgag gaagtcagac
      721 tattgatgaa tcattgtctg aattggctga acaaatgact attgaagaaa acagcagcaa
      781 ggataatgag cctattgtga agaatggcag gcctccaacg tctcacagta tgcattcctt
      841 cctccaccag tacacaggat ctttcaagaa gcccccattg cggagaccac acagtgttat
      901 tggagggagc cttggctcct tcatggcaat gcccagaaat ggaagcagat tagatattgt
      961 tcatgaaaat ctaaaaatgg gatcagatgg tgagagtgac caagcttctg ggacatcatc
     1021 tgatgaagtc cagtcaccta caggtgtttg tctcagaaat cgtatacata gacggatctc
     1081 aatggaggat ttaaataagc ggttatcact gcctgcagac atcagaatac ctgatggata
     1141 tcttgaaaag ttgcagataa acagtccacc atttgaccaa ccaatgagtc gaaggtctca
     1201 tagagcttcc ttatcagaaa ttggctttgg aaaaatggaa acctacatca aattggaaaa
     1261 gcttggagag ggtacatatg caacagtata taaaggaaga agtaaattga cagagaattt
     1321 ggtggcatta aaagagatcc gattggaaca tgaagaaggt gcaccctgca cagctataag
     1381 agaagtttca ctattaaagg atttaaaaca tgcaaatata gtaaccttac atgacattgt
     1441 tcacacagat aaatccttga ctttggtgtt tgagtatctg gataaagacc tgaaacagta
     1501 catggatgac tgtggaaaca tcatgagtat gcacaacgta aagctgtttc tgtaccaaat
     1561 tctacgtggt ttggcatatt gccatagaag aaaggtattg catcgagact tgaaaccaca
     1621 gaacctcctc attaatgaga aaggagaatt aaagctagca gattttggac tagcccgagc
     1681 caagtcagtt cccacaaaga cctactcaaa tgaagttgtc acactatggt accggccacc
     1741 tgatgtgctt cttggttcct cggagtactc aacacagatt gacatgtggg gtgttggttg
     1801 cattttcttt gaaatggctt ctggaagacc tttatttcca ggatcaaccg tggaagatga
     1861 actgcactta attttccgac tgctaggaac tccatctcag gaaacttggc caggtatttc
     1921 ttcaaatgag gagttcaaga actacaactt tccaaaatat aaaccacagc ctctaattaa
     1981 ccacgcaccc aggttagact ctgaaggaat tgagttgata acaaaatttc ttcagtatga
     2041 atctaagaaa agggtttcag ctgaagaggc catgaaacat gtgtactttc gaagtctggg
     2101 accaagaata catgctttac cagaaagtgt atcaatattc agtttgaaag agattcagtt
     2161 gcaaaaggac ccgggttttc gaaattcttc ttatccagag acaggacatg ggaagaacag
     2221 aagacagagc atgctctttt gattgccaac tttcttgtac aaagttggca ttataagaaa
     2281 gcattgctta tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata aaatcattat
     2341 ttgccatcca gctgatatcc cctatagtga gtcgtattac atggtcatag ctgtttcctg
     2401 gcagctctgg cccgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc
     2461 atcatgcctc ctctagacca gccaggacag aaatgcctcg acttcgctgc tacccaaggt
     2521 tgccgggtga cgcacaccgt ggaaacggat gaaggcacga acccagtgga cataagcctg
     2581 ttcggttcgt aagctgtaat gcaagtagcg tatgcgctca cgcaactggt ccagaacctt
     2641 gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt atgactgttt
     2701 ttttggggta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg
     2761 atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa
     2821 gttaaacatt atgagggaag cggtgatcgc cgaagtatcg actcaactat cagaggtagt
     2881 tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt acggctccgc
     2941 agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg tgaccgtaag
     3001 gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt cggcttcccc
     3061 tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg acgacatcat
     3121 tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc gcaatgacat
     3181 tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct tgctgacaaa
     3241 agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct ttgatccggt
     3301 tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga actcgccgcc
     3361 cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc
     3421 agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc
     3481 ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac aagaagaaga
     3541 tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc cactacgtga aaggcgagat
     3601 caccaaggta gtcggcaaat aaccctcgag ccacccatga ccaaaatccc ttaacgtgag
     3661 ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     3721 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     3781 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     3841 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     3901 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     3961 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     4021 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     4081 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     4141 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     4201 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     4261 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     4321 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgtt
//
