LOCUS       sequence-167255-       12902 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  target  miRNA393  for destruction in Arabidopsis thaliana.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12902)
  AUTHORS   Tang G, Yan J, Gu Y, Qiao M, Fan R, Mao Y, Tang X
  TITLE     Construction of short tandem target mimic (STTM) to block the 
            functions of plant and animal microRNAs.
  JOURNAL   Methods. 2012 Oct;58(2):118-25. doi: 10.1016/j.ymeth.2012.10.006. 
            Epub 2012 Oct 23.
  PUBMED    23098881
REFERENCE   2  (bases 1 to 12902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ymeth.2012.10.006"; journalName: "Methods"; date: 
            "2012-10"; volume: "58"; issue: "2"; pages: "118-25"
COMMENT     Sequence Label: pFGC5941DR5-PacI-ath-STTM393
FEATURES             Location/Qualifiers
     source          1..12902
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..346
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    507..683
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(1245..1263)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1246..1263)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     1902..1921
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(2024..2029,2030..2046,2047..2052))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     terminator      complement(2329..2581)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(2616..2633)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(join(2640..4457,4648..4662))
                     /codon_start=1
                     /product="beta-glucuronidase"
                     /label=GUS
                     /note="This version of the gusA gene has a 5' extension 
                     with a catalase intron to ensure expression in plants but 
                     not bacteria."
                     /translation="MVDLRNRRLVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWES
                     ALQESRAIAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGK
                     VWVNNQEVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGK
                     KKQSYFHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSV
                     ELRDADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRS
                     VAVKGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYP
                     YAEEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQA
                     IKELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDT
                     LAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKK
                     GIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     intron          4458..4647
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
     promoter        complement(4681..4727)
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     primer_bind     complement(4816..4833)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4816..4832)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4825..4847)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    5035..5059
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6360..6989
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             7418..8491
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      8557..8751
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(8987..9009)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    9095..9235
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     9150..9169
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(9250..9267)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9421..10009)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10096..10890)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    11315..11339
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(11425..11677)
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     CDS             complement(11687..12238)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(12244..12624)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     primer_bind     12816..12835
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        12816..12834
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tgagactttt caacaaaggg taatttcggg aaacctcctc ggattccatt gcccagctat
       61 ctgtcacttc atcgaaagga cagtggaaaa ggaaggtggc tcctacaaat gccatcattg
      121 cgataaagga aaggctatca ttcaagatgc ctctgccgac agtggtccca aagatggacc
      181 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
      241 ggattgatgt gacatctcca ctgacataag ggatgacgca caaccccact atccttcgca
      301 agacccttcc tctatataag gaagttcatt tcatttggag aggacagccc aagcttgatc
      361 aatgcgacta tccctttgga gttgttgttg ttatggtcta atttaaatat ggtctaaaga
      421 agaagaatga tcaatgcgac tatccctttg gagaattcgg tacgctgaaa tcaccagtct
      481 ctctctacaa atctatctct ctctattttc tccataaata atgtgtgagt agtttcccga
      541 taagggaaat tagggttctt atagggtttc gctcatgtgt cgagcatata agaaaccctt
      601 agtatgtatt tgtatttgta aaatacttct atcaataaaa tttctaattc ctaaaaccaa
      661 aatccagtac taaaatccag atctcctaaa gtccctatag atctttgtcg tgaatataaa
      721 ccagacacga gacgactaga cctggagccc agacgccgtt cgaagctaga agtaccgctt
      781 aggcaggagg ccgttaggga aaagatgcta aggcagggtt ggttacgttg actcccccgt
      841 aggtttggtt taaatatgat gaagtggacg gaaggaagga ggaagacaag gaaggataag
      901 gttgcaggcc ctgtgcaagg taagaagatg gaaatttgat agaggtacgc tactatactt
      961 atactatacg ctaagggaat gattgtattt ataccctata ccccctaata accccttatc
     1021 aatttaagaa ataatccgca taagcccccg cttaaaaatt ggtatcagag ccatgaatag
     1081 gtctatgacc aaaactcaag aggataaaac ctcaccaaaa tacgaaagag tccttaactc
     1141 taaagataaa agatctttca agatcaaaac tagttccctc acaccggtga cggggatcgc
     1201 atgcgacctc tagaccatca cattggagct tcagctgctc gagttctata gtgtcaccta
     1261 aatcgtatgt gtatgataca taaggttatg tattaattgt agccgcgttc ttacgtcgac
     1321 gtaccaatta cgccccgccc tgtcactcat cgcagtactg ttgtaattca ttaagcattc
     1381 tgccgacatg gaagccatca caaacggcat gatgaacctg aatcgccagc ggcatcagca
     1441 ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca
     1501 tattggccac gtttaaatca aaactggtga aactcaccca gggattggct gagacgaaaa
     1561 acatattctc aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat
     1621 cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg
     1681 aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatta
     1741 ccagctcacc gtctttcatt gccatacgga actccggatg agcattcatc aggcgggcaa
     1801 gaatgtgaat aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg
     1861 ccgtaatatc cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct
     1921 caaaatgttc tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttctt
     1981 tctccatttt agcttcctta gctcctgaaa atctcgataa ctcattaaag cttatcgatg
     2041 ataagctgtc aaacatgaga atttagcccg cctaatgagc gggctttttg ttgtgatccg
     2101 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     2161 caagtcagag gtggcgaaac ccgacaggac tataaaggta ccaggcgttt cccccctgga
     2221 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgctttc
     2281 ttaattaacc gccaatatat cctgtcaaac actgatagtt taattcccga tctagtaaca
     2341 tagatgacac cgcgcgcgat aatttatcct agtttgcgcg ctatattttg ttttctatcg
     2401 cgtattaaat gtataattgc gggactctaa tcataaaaac ccatctcata aataacgtca
     2461 tgcattacat gttaattatt acatgcttaa cgtaattcaa cagaaattat atgataatca
     2521 tcgcaagacc ggcaacagga ttcaatctta agaaacttta ttgccaaatg tttgaacgat
     2581 cggggaaatt cgagctggtc acctgtaatt cacacgtggt ggtggtggtg gtggctagct
     2641 tgtttgcctc cctgctgcgg tttttcaccg aagttcatgc cagtccagcg tttttgcagc
     2701 agaaaagccg ccgacttcgg tttgcggtcg cgagtgaaga tccctttctt gttaccgcca
     2761 acgcgcaata tgccttgcga ggtcgcaaaa tcggcgaaat tccatacctg ttcaccgacg
     2821 acggcgctga cgcgatcaaa gacgcggtga tacatatcca gccatgcaca ctgatactct
     2881 tcactccaca tgtcggtgta cattgagtgc agcccggcta acgtatccac gccgtattcg
     2941 gtgatgataa tcggctgatg cagtttctcc tgccaggcca gaagttcttt ttccagtacc
     3001 ttctctgccg tttccaaatc gccgctttgg acataccatc cgtaataacg gttcaggcac
     3061 agcacatcaa agagatcgct gatggtatcg gtgtgagcgt cgcagaacat tacattgacg
     3121 caggtgatcg gacgcgtcgg gtcgagttta cgcgttgctt ccgccagtgg cgcgaaatat
     3181 tcccgtgcac cttgcggacg ggtatccggt tcgttggcaa tactccacat caccacgctt
     3241 gggtggtttt tgtcacgcgc tatcagctct ttaatcgcct gtaagtgcgc ttgctgagtt
     3301 tccccgttga ctgcctcttc gctgtacagt tctttcggct tgttgcccgc ttcgaaacca
     3361 atgcctaaag agaggttaaa gccgacagca gcagtttcat caatcaccac gatgccatgt
     3421 tcatctgccc agtcgagcat ctcttcagcg taagggtaat gcgaggtacg gtaggagttg
     3481 gccccaatcc agtccattaa tgcgtggtcg tgcaccatca gcacgttatc gaatcctttg
     3541 ccacgcaagt ccgcatcttc atgacgacca aagccagtaa agtagaacgg tttgtggtta
     3601 atcaggaact gttcgccctt cactgccact gaccggatgc cgacgcgaag cgggtagata
     3661 tcacactctg tctggctttt ggctgtgacg cacagttcat agagataacc ttcacccggt
     3721 tgccagaggt gcggattcac cacttgcaaa gtcccgctag tgccttgtcc agttgcaacc
     3781 acctgttgat ccgcatcacg cagttcaacg ctgacatcac cattggccac cacctgccag
     3841 tcaacagacg cgtggttaca gtcttgcgcg acatgcgtca ccacggtgat atcgtccacc
     3901 caggtgttcg gcgtggtgta gagcattacg ctgcgatgga ttccggcata gttaaagaaa
     3961 tcatggaagt aagactgctt tttcttgccg ttttcgtcgg taatcaccat tcccggcggg
     4021 atagtctgcc agttcagttc gttgttcaca caaacggtga tacgtacact tttcccggca
     4081 ataacatacg gcgtgacatc ggcttcaaat ggcgtatagc cgccctgatg ctccatcact
     4141 tcctgattat tgacccacac tttgccgtaa tgagtgaccg catcgaaacg cagcacgata
     4201 cgctggcctg cccaaccttt cggtataaag acttcgcgct gataccagac gttgcccgca
     4261 taattacgaa tatctgcatc ggcgaactga tcgttaaaac tgcctggcac agcaattgcc
     4321 cggctttctt gtaacgcgct ttcccaccaa cgctgatcaa ttccacagtt ttcgcgatcc
     4381 agactgaatg cccacaggcc gtcgagtttt ttgatttcac gggttggggt ttctacagga
     4441 cggacgagtc gtcggttctg taactatcat catcatcata gacacacgaa ataaagtaat
     4501 cagattatca gttaaagcta tgtaatattt acaccataac caatcaatta aaaaatagat
     4561 cagtttaaag aaagatcaaa gctcaaaaaa ataaaaagag aaaagggtcc taaccaagaa
     4621 aatgaaggag aaaaactaga aatttaccct cagatctacc atggtcaaga gtcccccgtg
     4681 ttctctccaa atgaaatgaa cttccttata tagaggaagg gtcttgcgag agacaaaagg
     4741 gagacaaaag ggagacaaaa gggagacaaa agggagacaa aagggagaca aaagggagac
     4801 aaaaggaagc ttggcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     4861 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     4921 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gctagagcag
     4981 cttgagcttg gatcagattg tcgtttcccg ccttcagttt aaactatcag tgtttgacag
     5041 gatatattgg cgggtaaacc taagagaaaa gagcgtttat tagaataatc ggatatttaa
     5101 aagggcgtga aaaggtttat ccgttcgtcc atttgtatgt gcatgccaac cacagggttc
     5161 ccctcgggat caaagtactt tgatccaacc cctccgctgc tatagtgcag tcggcttctg
     5221 acgttcagtg cagccgtctt ctgaaaacga catgtcgcac aagtcctaag ttacgcgaca
     5281 ggctgccgcc ctgccctttt cctggcgttt tcttgtcgcg tgttttagtc gcataaagta
     5341 gaatacttgc gactagaacc ggagacatta cgccatgaac aagagcgccg ccgctggcct
     5401 gctgggctat gcccgcgtca gcaccgacga ccaggacttg accaaccaac gggccgaact
     5461 gcacgcggcc ggctgcacca agctgttttc cgagaagatc accggcacca ggcgcgaccg
     5521 cccggagctg gccaggatgc ttgaccacct acgccctggc gacgttgtga cagtgaccag
     5581 gctagaccgc ctggcccgca gcacccgcga cctactggac attgccgagc gcatccagga
     5641 ggccggcgcg ggcctgcgta gcctggcaga gccgtgggcc gacaccacca cgccggccgg
     5701 ccgcatggtg ttgaccgtgt tcgccggcat tgccgagttc gagcgttccc taatcatcga
     5761 ccgcacccgg agcgggcgcg aggccgccaa ggcccgaggc gtgaagtttg gcccccgccc
     5821 taccctcacc ccggcacaga tcgcgcacgc ccgcgagctg atcgaccagg aaggccgcac
     5881 cgtgaaagag gcggctgcac tgcttggcgt gcatcgctcg accctgtacc gcgcacttga
     5941 gcgcagcgag gaagtgacgc ccaccgaggc caggcggcgc ggtgccttcc gtgaggacgc
     6001 attgaccgag gccgacgccc tggcggccgc cgagaatgaa cgccaagagg aacaagcatg
     6061 aaaccgcacc aggacggcca ggacgaaccg tttttcatta ccgaagagat cgaggcggag
     6121 atgatcgcgg ccgggtacgt gttcgagccg cccgcgcacg tctcaaccgt gcggctgcat
     6181 gaaatcctgg ccggtttgtc tgatgccaag ctggcggcct ggccggccag cttggccgct
     6241 gaagaaaccg agcgccgccg tctaaaaagg tgatgtgtat ttgagtaaaa cagcttgcgt
     6301 catgcggtcg ctgcgtatat gatgcgatga gtaaataaac aaatacgcaa ggggaacgca
     6361 tgaaggttat cgctgtactt aaccagaaag gcgggtcagg caagacgacc atcgcaaccc
     6421 atctagcccg cgccctgcaa ctcgccgggg ccgatgttct gttagtcgat tccgatcccc
     6481 agggcagtgc ccgcgattgg gcggccgtgc gggaagatca accgctaacc gttgtcggca
     6541 tcgaccgccc gacgattgac cgcgacgtga aggccatcgg ccggcgcgac ttcgtagtga
     6601 tcgacggagc gccccaggcg gcggacttgg ctgtgtccgc gatcaaggca gccgacttcg
     6661 tgctgattcc ggtgcagcca agcccttacg acatatgggc caccgccgac ctggtggagc
     6721 tggttaagca gcgcattgag gtcacggatg gaaggctaca agcggccttt gtcgtgtcgc
     6781 gggcgatcaa aggcacgcgc atcggcggtg aggttgccga ggcgctggcc gggtacgagc
     6841 tgcccattct tgagtcccgt atcacgcagc gcgtgagcta cccaggcact gccgccgccg
     6901 gcacaaccgt tcttgaatca gaacccgagg gcgacgctgc ccgcgaggtc caggcgctgg
     6961 ccgctgaaat taaatcaaaa ctcatttgag ttaatgaggt aaagagaaaa tgagcaaaag
     7021 cacaaacacg ctaagtgccg gccgtccgag cgcacgcagc agcaaggctg caacgttggc
     7081 cagcctggca gacacgccag ccatgaagcg ggtcaacttt cagttgccgg cggaggatca
     7141 caccaagctg aagatgtacg cggtacgcca aggcaagacc attaccgagc tgctatctga
     7201 atacatcgcg cagctaccag agtaaatgag caaatgaata aatgagtaga tgaattttag
     7261 cggctaaagg aggcggcatg gaaaatcaag aacaaccagg caccgacgcc gtggaatgcc
     7321 ccatgtgtgg aggaacgggc ggttggccag gcgtaagcgg ctgggttgcc tgccggccct
     7381 gcaatggcac tggaaccccc aagcccgagg aatcggcgtg agcggtcgca aaccatccgg
     7441 cccggtacaa atcggcgcgg cgctgggtga tgacctggtg gagaagttga aggccgcgca
     7501 ggccgcccag cggcaacgca tcgaggcaga agcacgcccc ggtgaatcgt ggcaagcggc
     7561 cgctgatcga atccgcaaag aatcccggca accgccggca gccggtgcgc cgtcgattag
     7621 gaagccgccc aagggcgacg agcaaccaga ttttttcgtt ccgatgctct atgacgtggg
     7681 cacccgcgat agtcgcagca tcatggacgt ggccgttttc cgtctgtcga agcgtgaccg
     7741 acgagctggc gaggtgatcc gctacgagct tccagacggg cacgtagagg tttccgcagg
     7801 gccggccggc atggccagtg tgtgggatta cgacctggta ctgatggcgg tttcccatct
     7861 aaccgaatcc atgaaccgat accgggaagg gaagggagac aagcccggcc gcgtgttccg
     7921 tccacacgtt gcggacgtac tcaagttctg ccggcgagcc gatggcggaa agcagaaaga
     7981 cgacctggta gaaacctgca ttcggttaaa caccacgcac gttgccatgc agcgtacgaa
     8041 gaaggccaag aacggccgcc tggtgacggt atccgagggt gaagccttga ttagccgcta
     8101 caagatcgta aagagcgaaa ccgggcggcc ggagtacatc gagatcgagc tagctgattg
     8161 gatgtaccgc gagatcacag aaggcaagaa cccggacgtg ctgacggttc accccgatta
     8221 ctttttgatc gatcccggca tcggccgttt tctctaccgc ctggcacgcc gcgccgcagg
     8281 caaggcagaa gccagatggt tgttcaagac gatctacgaa cgcagtggca gcgccggaga
     8341 gttcaagaag ttctgtttca ccgtgcgcaa gctgatcggg tcaaatgacc tgccggagta
     8401 cgatttgaag gaggaggcgg ggcaggctgg cccgatccta gtcatgcgct accgcaacct
     8461 gatcgagggc gaagcatccg ccggttccta atgtacggag cagatgctag ggcaaattgc
     8521 cctagcaggg gaaaaaggtc gaaaaggtct ctttcctgtg gatagcacgt acattgggaa
     8581 cccaaagccg tacattggga accggaaccc gtacattggg aacccaaagc cgtacattgg
     8641 gaaccggtca cacatgtaag tgactgatat aaaagagaaa aaaggcgatt tttccgccta
     8701 aaactcttta aaacttatta aaactcttaa aacccgcctg gcctgtgcat aactgtctgg
     8761 ccagcgcaca gccgaagagc tgcaaaaagc gcctaccctt cggtcgctgc gctccctacg
     8821 ccccgccgct tcgcgtcggc ctatcgcggc cgctggccgc tcaaaaatgg ctggcctacg
     8881 gccaggcaat ctaccagggc gcggacaagc cgcgccgtcg ccactcgacc gccggcgccc
     8941 acatcaaggc accctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc
     9001 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc
     9061 agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg
     9121 atagcggagt gtatactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
     9181 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc
     9241 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     9301 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     9361 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     9421 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     9481 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     9541 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     9601 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     9661 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     9721 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     9781 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     9841 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     9901 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     9961 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
    10021 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
    10081 catgcattct aggtactaaa acaattcatc cagtaaaata taatatttta ttttctccca
    10141 atcaggcttg atccccagta agtcaaaaaa tagctcgaca tactgttctt ccccgatatc
    10201 ctccctgatc gaccggacgc agaaggcaat gtcataccac ttgtccgccc tgccgcttct
    10261 cccaagatca ataaagccac ttactttgcc atctttcaca aagatgttgc tgtctcccag
    10321 gtcgccgtgg gaaaagacaa gttcctcttc gggcttttcc gtctttaaaa aatcatacag
    10381 ctcgcgcgga tctttaaatg gagtgtcttc ttcccagttt tcgcaatcca catcggccag
    10441 atcgttattc agtaagtaat ccaattcggc taagcggctg tctaagctat tcgtataggg
    10501 acaatccgat atgtcgatgg agtgaaagag cctgatgcac tccgcataca gctcgataat
    10561 cttttcaggg ctttgttcat cttcatactc ttccgagcaa aggacgccat cggcctcact
    10621 catgagcaga ttgctccagc catcatgccg ttcaaagtgc aggacctttg gaacaggcag
    10681 ctttccttcc agccatagca tcatgtcctt ttcccgttcc acatcatagg tggtcccttt
    10741 ataccggctg tccgtcattt ttaaatatag gttttcattt tctcccacca gcttatatac
    10801 cttagcagga gacattcctt ccgtatcttt tacgcagcgg tatttttcga tcagtttttt
    10861 caattccggt gatattctca ttttagccat ttattatttc cttcctcttt tctacagtat
    10921 ttaaagatac cccaagaagc taattataac aagacgaact ccaattcact gttccttgca
    10981 ttctaaaacc ttaaatacca gaaaacagct ttttcaaagt tgttttcaaa gttggcgtat
    11041 aacatagtat cgacggagcc gattttgaaa ccgcggtgat cacaggcagc aacgctctgt
    11101 catcgttaca atcaacatgc taccctccgc gagatcatcc gtgtttcaaa cccggcagct
    11161 tagttgccgt tcttccgaat agcatcggta acatgagcaa agtctgccgc cttacaacgg
    11221 ctctcccgct gacgccgtcc cggactgatg ggctgcctgt atcgagtggt gattttgtgc
    11281 cgagctgccg gtcggggagc tgttggctgg ctggtggcag gatatattgt ggtgtaaaca
    11341 aattgacgct tagacaactt aataacacat tgcggacgtt tttaatgtac tgaattaacg
    11401 ccgaattaat tcgagctcgg atctgataat ttatttgaaa attcataaga aaagcaaacg
    11461 ttacatgaat tgatgaaaca atacaaagac agataaagcc acgcacattt aggatattgg
    11521 ccgagattac tgaatattga gtaagatcac ggaatttctg acaggagcat gtcttcaatt
    11581 cagcccaaat ggcagttgaa atactcaaac cgccccatat gcaggagcgg atcattcatt
    11641 gtttgtttgg ttgcctttgc caacatggga gtccaagatt ctgcagtcaa atctcggtga
    11701 cgggcaggac cggacggggc ggtaccggca ggctgaagtc cagctgccag aaacccacgt
    11761 catgccagtt cccgtgcttg aagccggccg cccgcagcat gccgcggggg gcatatccga
    11821 gcgcctcgtg catgcgcacg ctcgggtcgt tgggcagccc gatgacagcg accacgctct
    11881 tgaagccctg tgcctccagg gacttcagca ggtgggtgta gagcgtggag cccagtcccg
    11941 tccgctggtg gcggggggag acgtacacgg tcgactcggc cgtccagtcg taggcgttgc
    12001 gtgccttcca ggggcccgcg taggcgatgc cggcgacctc gccgtccacc tcggcgacga
    12061 gccagggata gcgctcccgc agacggacga ggtcgtccgt ccactcctgc ggttcctgcg
    12121 gctcggtacg gaagttgacc gtgcttgtct cgatgtagtg gttgacgatg gtgcagaccg
    12181 ccggcatgtc cgcctcggtg gcacggcgga tgtcggccgg gcgtcgttct gggctcatcg
    12241 attcgatttg gtgtatcgag attggttatg aaattcagat gctagtgtaa tgtattggta
    12301 atttgggaag atataatagg aagcaaggct atttatccat ttctgaaaag gcgaaatggc
    12361 gtcaccgcga gcgtcacgcg cattccgttc ttgctgtaaa gcgttgtttg gtacactttt
    12421 gactagcgag gcttggcgtg tcagcgtatc tattcaaaag tcgttaatgg ctgcggatca
    12481 agaaaaagtt ggaatagaaa cagaataccc gcgaaattca ggcccggttg ccatgtccta
    12541 cacgccgaaa taaacgacca aattagtaga aaaataaaaa ctgactcgga tacttacgtc
    12601 acgtcttgcg cactgatttg aaaaatctca gaattcatgg atcctaggtg agtctagaga
    12661 gttaattaag tttgcaagca gcagattacg cgcagaaaaa aaggatctca aggagatcct
    12721 ttggatcaca aaaaaaaagc ccgctcatta ggcgggttaa attcccgggt actgcagccg
    12781 attcattaat gcaggttaac ctggcttatc gaaattaata cgactcacta tagggagacc
    12841 ggcagatctg atccctactc caaaaatgtc aaagatacag tctcagaaga ccaaagggct
    12901 at
//
