LOCUS       sequence-167260-        1072 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For AAV-TRE-Cre based Supernova, pK170 should be used with pK168 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1072)
  AUTHORS   Luo W, Mizuno H, Iwata R, Nakazawa S, Yasuda K, Itohara S, Iwasato T
  TITLE     Supernova: A Versatile Vector System for Single-Cell Labeling and 
            Gene Function Studies in vivo.
  JOURNAL   Sci Rep. 2016 Oct 24;6:35747. doi: 10.1038/srep35747.
  PUBMED    27775045
REFERENCE   2  (bases 1 to 1072)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-10-24"; volume: "6:35747. doi"; pages: " 
            10.1038/srep35747"
COMMENT     Sequence Label: pK170.AAV-TRE-Cre-WPRE
            (Supernova)
FEATURES             Location/Qualifiers
     source          1..1072
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             18..38
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             39..1067
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="SNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKMLL
                     SVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLP
                     RPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFL
                     GIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVER
                     WISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQR
                     YLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLED
                     GD"
ORIGIN
        1 cactgtcgac catgggccca aagaagaaga gaaaggtttc gaatttactg accgtacacc
       61 aaaatttgcc tgcattaccg gtcgatgcaa cgagtgatga ggttcgcaag aacctgatgg
      121 acatgttcag ggatcgccag gcgttttctg agcatacctg gaaaatgctt ctgtccgttt
      181 gccggtcgtg ggcggcatgg tgcaagttga ataaccggaa atggtttccc gcagaacctg
      241 aagatgttcg cgattatctt ctatatcttc aggcgcgcgg tctggcagta aaaactatcc
      301 agcaacattt gggccagcta aacatgcttc atcgtcggtc cgggctgcca cgaccaagtg
      361 acagcaatgc tgtttcactg gttatgcggc ggatccgaaa agaaaacgtt gatgccggtg
      421 aacgtgcaaa acaggctcta gcgttcgaac gcactgattt cgaccaggtt cgttcactca
      481 tggaaaatag cgatcgctgc caggatatac gtaatctggc atttctgggg attgcttata
      541 acaccctgtt acgtatagcc gaaattgcca ggatcagggt taaagatatc tcacgtactg
      601 acggtgggag aatgttaatc catattggca gaacgaaaac gctggttagc accgcaggtg
      661 tagagaaggc acttagcctg ggggtaacta aactggtcga gcgatggatt tccgtctctg
      721 gtgtagctga tgatccgaat aactacctgt tttgccgggt cagaaaaaat ggtgttgccg
      781 cgccatctgc caccagccag ctatcaactc gcgccctgga agggattttt gaagcaactc
      841 atcgattgat ttacggcgct aaggatgact ctggtcagag atacctggcc tggtctggac
      901 acagtgcccg tgtcggagcc gcgcgagata tggcccgcgc tggagtttca ataccggaga
      961 tcatgcaagc tggtggctgg accaatgtaa atattgtcat gaactatatc cgtaacctgg
     1021 atagtgaaac aggggcaatg gtgcgcctgc tggaagatgg cgattaggga ta
//
