LOCUS       sequence-167261-        1081 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For AAV-TRE-Cre based Supernova, pK170 should be used with pK168 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Luo W, Mizuno H, Iwata R, Nakazawa S, Yasuda K, Itohara S, Iwasato T
  TITLE     Supernova: A Versatile Vector System for Single-Cell Labeling and 
            Gene Function Studies in vivo.
  JOURNAL   Sci Rep. 2016 Oct 24;6:35747. doi: 10.1038/srep35747.
  PUBMED    27775045
REFERENCE   2  (bases 1 to 1081)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-10-24"; volume: "6:35747. doi"; pages: " 
            10.1038/srep35747"
COMMENT     Sequence Label: pK170.AAV-TRE-Cre-WPRE
            (Supernova)
FEATURES             Location/Qualifiers
     source          1..1081
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    215..803
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             321..332
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     730..750
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 ggttgtcttc ccaacttgcc ccttgctcca taccaccccc ctccacccca taatattata
       61 gaaggacacc tagtcagaca aaatgatgca acttaatttt attaggacaa ggctggtggg
      121 cactggagtg gcaacttcca gggccaggag aggcactggg gaggggtcac agggatgcca
      181 cccgtagatc tctcgagcag cgctcggtat cgatgcgggg aggcggccca aagggagatc
      241 cgactcgtct gagggcgaag gcgaagacgc ggaagaggcc gcagagccgg cagcaggccg
      301 cgggaaggaa ggtccgctgg attgagggcc gaagggacgt agcagaagga cgtcccgcgc
      361 agaatccagg tggcaacata ggcgagcagc caaggaaagg acgatgattt ccccgacaac
      421 accacggaat tgtcagtgcc caacagccga gcccctgtcc agcagcgggc aaggcaggcg
      481 gcgatgagtt ccgccgtggc aatagggagg gggaaagcga aagtcccgga aaggagctga
      541 caggtggtgg caatgcccca accagtgggg gttgcgtcag caaacacagt gcacaccacg
      601 ccacgttgcc tgacaacggg ccacaactcc tcataaagag acagcaacca ggatttatac
      661 aaggaggaga aaatgaaagc catacgggaa gcaatagcat gatacaaagg cattaaagca
      721 gcgtatccac atagcgtaaa aggagcaaca tagttaagaa taccagtcaa tctttcacaa
      781 attttgtaat ccagaggttg attatcgata agcttgtcga cgctagcgga tctgatatcc
      841 ctaatcgcca tcttccagca ggcgcaccat tgcccctgtt tcactatcca ggttacggat
      901 atagttcatg acaatattta cattggtcca gccaccagct tgcatgatct ccggtattga
      961 aactccagcg cgggccatat ctcgcgcggc tccgacacgg gcactgtgtc cagaccaggc
     1021 caggtatctc tgaccagagt catccttagc gccgtaaatc aatcgatgag ttgcttcaaa
     1081 a
//
