LOCUS       sequence-167267-         896 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid should be used for Cre/loxP-based 
            Supernova-CRISPR/Cas9..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Luo W, Mizuno H, Iwata R, Nakazawa S, Yasuda K, Itohara S, Iwasato T
  TITLE     Supernova: A Versatile Vector System for Single-Cell Labeling and 
            Gene Function Studies in vivo.
  JOURNAL   Sci Rep. 2016 Oct 24;6:35747. doi: 10.1038/srep35747.
  PUBMED    27775045
REFERENCE   2  (bases 1 to 896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-10-24"; volume: "6:35747. doi"; pages: " 
            10.1038/srep35747"
COMMENT     Sequence Label: pK237.U6-Chimeric-CAG-loxP-stop-loxP-hspCas9
            (...
FEATURES             Location/Qualifiers
     source          1..896
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(47..80)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      116..125
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             125..190
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             197..217
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ggatctgaca tggtaagtaa gcttgggctg caggtcgagg gacctaataa cttcgtatag
       61 catacattat acgaagttat atgtcgacgg taccgcgggc ccgggatcca ccggtgccac
      121 catggactat aaggaccacg acggagacta caaggatcat gatattgatt acaaagacga
      181 tgacgataag atggccccaa agaagaagcg gaaggtcggt atccacggag tcccagcagc
      241 cgacaagaag tacagcatcg gcctggacat cggcaccaac tctgtgggct gggccgtgat
      301 caccgacgag tacaaggtgc ccagcaagaa attcaaggtg ctgggcaaca ccgaccggca
      361 cagcatcaag aagaacctga tcggagccct gctgttcgac agcggcgaaa cagccgaggc
      421 cacccggctg aagagaaccg ccagaagaag atacaccaga cggaagaacc ggatctgcta
      481 tctgcaagag atcttcagca acgagatggc caaggtggac gacagcttct tccacagact
      541 ggaagagtcc ttcctggtgg aagaggataa gaagcacgag cggcacccca tcttcggcaa
      601 catcgtggac gaggtggcct accacgagaa gtaccccacc atctaccacc tgagaaagaa
      661 actggtggac agcaccgaca aggccgacct gcggctgatc tatctggccc tggcccacat
      721 gatcaagttc cggggccact tcctgatcga gggcgacctg aaccccgaca acagcgacgt
      781 ggacaagctg ttcatccagc tggtgcagac ctacaaccag ctgttcgagg aaaaccccat
      841 caacgccagc ggcgtggacg ccaaggccat cctgtctgcc agactgagca agagca
//
