LOCUS       sequence-167270-        1010 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid should be used for Cre/loxP-based 
            Supernova-CRISPR/Cas9..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Luo W, Mizuno H, Iwata R, Nakazawa S, Yasuda K, Itohara S, Iwasato T
  TITLE     Supernova: A Versatile Vector System for Single-Cell Labeling and 
            Gene Function Studies in vivo.
  JOURNAL   Sci Rep. 2016 Oct 24;6:35747. doi: 10.1038/srep35747.
  PUBMED    27775045
REFERENCE   2  (bases 1 to 1010)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-10-24"; volume: "6:35747. doi"; pages: " 
            10.1038/srep35747"
COMMENT     Sequence Label: pK237.U6-Chimeric-CAG-loxP-stop-loxP-hspCas9
            (...
FEATURES             Location/Qualifiers
     source          1..1010
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(22..55)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     intron          589..654
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             784..804
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tcattttggc aaagaattct aataacttcg tatagcatac attatacgaa gttatattaa
       61 gggttccgga tcctcgggga caccaaatat ggcgatctcg gccttttcgt ttcttggagc
      121 tgggacatgt ttgccatcga tccatctacc accagaacgg ccgttagatc tgctgccacc
      181 gttgtttcca ccgaagaaac caccgttgcc gtaaccacca cgacggttgt tgctaaagaa
      241 gctgccaccg ccacggccac cgttgtagcc gccgttgttg ttattgtagt tgctactgtt
      301 atttctggca cttcttggtt ttcctcttaa gtgaggagga acataaccat tctcgttgtt
      361 gtcgttgatg cttaaatttt gcacttgttc gctcagttca gccataatat gaaatgcttt
      421 tcttgttgtt cttacggaat accacttgcc acctatcacc acaactaact ttttcccgtt
      481 cctccatctc ttttatattt tttttctcga gggatctttg tgaaggaacc ttacttctgt
      541 ggtgtgacat aattggacaa actacctaca gagatttaaa gctctaaggt aaatataaaa
      601 tttttaagtg tataatgtgt taaactactg attctaattg tttgtgtatt ttagattcca
      661 acctatggaa ctgatgaatg ggagcagtgg tggaatgcct ttaatgagga aaacctgttt
      721 tgctcagaag aaatgccatc tagtgatgat gaggctactg ctgactctca acattctact
      781 cctccaaaaa agaagagaaa ggtagaagac cccaaggact ttccttcaga attgctaagt
      841 tttttgagtc atgctgtgtt tagtaataga actcttgctt gctttgctat ttacaccaca
      901 aaggaaaaag ctgcactgct atacaagaaa attatggaaa aatattctgt aacctttata
      961 agtaggcata acagttataa tcataacata ctgttttttc ttactccaca
//
