LOCUS       sequence-167275-        4335 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing ACVR2A , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4335)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4335)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_ACVR2A_p.A381V
FEATURES             Location/Qualifiers
     source          1..4335
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2214..2313)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2330..2349)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2331..2349)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2354..2370)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2354..2370)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2801..3592
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3685..4273
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4174..4193
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcacc atgggagctg ctgcaaagtt ggcgtttgcc gtctttctta tctcctgttc
      721 ttcaggtgct atacttggta gatcagaaac tcaggagtgt cttttcttta atgctaattg
      781 ggaaaaagac agaaccaatc aaactggtgt tgaaccgtgt tatggtgaca aagataaacg
      841 gcggcattgt tttgctacct ggaagaatat ttctggttcc attgaaatag tgaaacaagg
      901 ttgttggctg gatgatatca actgctatga caggactgat tgtgtagaaa aaaaagacag
      961 ccctgaagta tatttttgtt gctgtgaggg caatatgtgt aatgaaaagt tttcttattt
     1021 tccagagatg gaagtcacac agcccacttc aaatccagtt acacctaagc caccctatta
     1081 caacatcctg ctctattcct tggtgccact tatgttaatt gcggggattg tcatttgtgc
     1141 attttgggtg tacaggcatc acaagatggc ctaccctcct gtacttgttc caactcaaga
     1201 cccaggacca cccccacctt ctccattact aggtttgaaa ccactgcagt tattagaagt
     1261 gaaagcaagg ggaagatttg gttgtgtctg gaaagcccag ttgcttaacg aatatgtggc
     1321 tgtcaaaata tttccaatac aggacaaaca gtcatggcaa aatgaatacg aagtctacag
     1381 tttgcctgga atgaagcatg agaacatatt acagttcatt ggtgcagaaa aacgaggcac
     1441 cagtgttgat gtggatcttt ggctgatcac agcatttcat gaaaagggtt cactatcaga
     1501 ctttcttaag gctaatgtgg tctcttggaa tgaactgtgt catattgcag aaaccatggc
     1561 tagaggattg gcatatttac atgaggatat acctggccta aaagatggcc acaaacctgc
     1621 catatctcac agggacatca aaagtaaaaa tgtgctgttg aaaaacaacc tgacagcttg
     1681 cattgctgac tttgggttgg ccttaaaatt tgaggctggc aagtctgcag gcgataccca
     1741 tggacaggtt ggtacccgga ggtacatggc tccagaggta ttagagggtg ctataaactt
     1801 ccaaagggat gtatttttga ggatagatat gtatgccatg ggattagtcc tatgggaact
     1861 ggcttctcgc tgtactgctg cagatggacc tgtagatgaa tacatgttgc catttgagga
     1921 ggaaattggc cagcatccat ctcttgaaga catgcaggaa gttgttgtgc ataaaaaaaa
     1981 gaggcctgtt ttaagagatt attggcagaa acatgctgga atggcaatgc tctgtgaaac
     2041 cattgaagaa tgttgggatc acgacgcaga agccaggtta tcagctggat gtgtaggtga
     2101 aagaattacc cagatgcaga gactaacaaa tattattacc acagaggaca ttgtaacagt
     2161 ggtcacaatg gtgacaaatg ttgactttcc tcccaaagaa tctagtctat gattgccaac
     2221 tttcttgtac aaagttggca ttataagaaa gcattgctta tcaatttgtt gcaacgaaca
     2281 ggtcactatc agtcaaaata aaatcattat ttgccatcca gctgatatcc cctatagtga
     2341 gtcgtattac atggtcatag ctgtttcctg gcagctctgg cccgtgtctc aaaatctctg
     2401 atgttacatt gcacaagata aaaatatatc atcatgcctc ctctagacca gccaggacag
     2461 aaatgcctcg acttcgctgc tacccaaggt tgccgggtga cgcacaccgt ggaaacggat
     2521 gaaggcacga acccagtgga cataagcctg ttcggttcgt aagctgtaat gcaagtagcg
     2581 tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta acggcgcagt
     2641 ggcggttttc atggcttgtt atgactgttt ttttggggta cagtctatgc ctcgggcatc
     2701 caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt
     2761 tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatt atgagggaag cggtgatcgc
     2821 cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac
     2881 gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc cacacagtga
     2941 tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc gagctttgat
     3001 caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc gcgctgtaga
     3061 agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta agcgcgaact
     3121 gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc cagccacgat
     3181 cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg ccttggtagg
     3241 tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg aggcgctaaa
     3301 tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc gaaatgtagt
     3361 gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt
     3421 cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca tacttgaagc
     3481 tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga
     3541 agaatttgtc cactacgtga aaggcgagat caccaaggta gtcggcaaat aaccctcgag
     3601 ccacccatga ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc
     3661 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     3721 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     3781 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     3841 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     3901 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     3961 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     4021 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     4081 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     4141 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     4201 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     4261 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     4321 ttttgctcac atgtt
//
