LOCUS       sequence-167279-         773 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  target  miRNA397  for destruction in Arabidopsis thaliana.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Tang G, Yan J, Gu Y, Qiao M, Fan R, Mao Y, Tang X
  TITLE     Construction of short tandem target mimic (STTM) to block the 
            functions of plant and animal microRNAs.
  JOURNAL   Methods. 2012 Oct;58(2):118-25. doi: 10.1016/j.ymeth.2012.10.006. 
            Epub 2012 Oct 23.
  PUBMED    23098881
REFERENCE   2  (bases 1 to 773)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ymeth.2012.10.006"; journalName: "Methods"; date: 
            "2012-10"; volume: "58"; issue: "2"; pages: "118-25"
COMMENT     Sequence Label: pFGC5941-PacI-ath-STTM397
FEATURES             Location/Qualifiers
     source          1..773
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     118..137
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        join(279..284,285..301,302..307)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(410..429)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 gaaatctcag aattcatgga tcctagggtg agtctagaga gttaattaag aaaggcggac
       61 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccagggggg
      121 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
      181 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggatcacaa
      241 caaaaagccc gctcattagg cgggctaaat tctcatgttt gacagcttat catcgataag
      301 ctttaatgag ttatcgagat tttcaggagc taaggaagct aaaatggaga aagaaatcac
      361 tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca
      421 gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa
      481 gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct
      541 gatgaatgct catccggagt tccgtatggc aatgaaagac ggtgagctgg taatatggga
      601 tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg
      661 gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg
      721 ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt ttt
//
