LOCUS       sequence-167286-        1080 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For AAV-TRE-Cre based Supernova-RFP, pK168 should be used with 
            pK170..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Luo W, Mizuno H, Iwata R, Nakazawa S, Yasuda K, Itohara S, Iwasato T
  TITLE     Supernova: A Versatile Vector System for Single-Cell Labeling and 
            Gene Function Studies in vivo.
  JOURNAL   Sci Rep. 2016 Oct 24;6:35747. doi: 10.1038/srep35747.
  PUBMED    27775045
REFERENCE   2  (bases 1 to 1080)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-10-24"; volume: "6:35747. doi"; pages: " 
            10.1038/srep35747"
COMMENT     Sequence Label: pK168.AAV-EF1-alpha-DIO-tTA-P2A-RFP-WPRE (Supernova)
FEATURES             Location/Qualifiers
     source          1..1080
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(48..81)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(132..165)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(174..869)
                     /codon_start=1
                     /product="red fluorescent protein from Entacmaea 
                     quadricolor"
                     /label=TurboRFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
                     VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA
                     TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK
                     LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC
                     DLPSKLGHR"
     CDS             complement(870..926)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(948..1058)
                     /codon_start=1
                     /product="3 tandem repeats of the minimal activation domain
                     of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP48
                     /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML"
ORIGIN
        1 catttcaggt gtcgtgaggt accggatcct ctagagtcga ctccggaata acttcgtata
       61 ggatacttta tacgaagtta tgcagaatgg tagctggatt gtagctgcta ttagcaatat
      121 gaaacctctt aataacttcg tatagcatac attatacgaa gttatggcgc gcctcatctg
      181 tgccccagtt tgctagggag gtcgcagtac ttggccacag ccatctcgtg ctgctcgacg
      241 taggtctctt tgtcggcctc cttgattctt tccagtctgt ggtccacgaa gtggaagccg
      301 ggcatcttga ggttcttagc gggtttcttg gatctgtatg tggtcttgaa ggagcagtgc
      361 aggtagcccc cgcccacgag cttcagggcc atctggctgt ggcctctcag gccgccgtca
      421 gcggggtaca gcatctcggt gttggcctcc cagccgcgtg ttttcttctg catcacaggg
      481 ccgttggatg ggaagttcac cccgttgatc ttgacgttgt agatgatgca gccgttctgg
      541 aagctggtgt cctgggtagc ggtcagcacg cccccgtctt cgtatgtggt gattctctcc
      601 catgtgaagc cctcagggaa ggactgctta aagaagtcgg ggatgccctg ggtgtggttg
      661 atgaaggctt tgctgccgta catgaagctg gtagccagga tgtcgaaggc gaaggggaga
      721 gggccgccct cgaccacctt gatcttcatg gtctgggtgc cctcgtaggg cttgccttcg
      781 ccctcggatg tgcacttgaa gtggtggttg ttcacggtgc cctccatgta cagcttcatg
      841 tgcatgttct ccttgatcag ctcgctcata ggtccagggt tctcctccac gtctccagcc
      901 tgcttcagca ggctgaagtt agtagctccg cttccggatc ccccggggag catgtcaagg
      961 tcaaaatcgt caagagcgtc agcaggcagc atatcaaggt caaagtcgtc aagggcatcg
     1021 gctgggagca tgtctaagtc aaaatcgtca agggcgtcgg ccggcccgcc gctttcgcac
//
