LOCUS       sequence-167292-       10265 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For Cre/loxP-based Supernova-GFP, pK038 should be used with pK031. 
            The GFP fragment of pK038 can be replaced with another gene by using
            SalI and EcoRV sites..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10265)
  AUTHORS   Luo W, Mizuno H, Iwata R, Nakazawa S, Yasuda K, Itohara S, Iwasato T
  TITLE     Supernova: A Versatile Vector System for Single-Cell Labeling and 
            Gene Function Studies in vivo.
  JOURNAL   Sci Rep. 2016 Oct 24;6:35747. doi: 10.1038/srep35747.
  PUBMED    27775045
REFERENCE   2  (bases 1 to 10265)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-10-24"; volume: "6:35747. doi"; pages: " 
            10.1038/srep35747"
COMMENT     Sequence Label: pK038.CAG-loxP-stop-loxP-EGFP-ires-tTA-WPRE
            (S...
FEATURES             Location/Qualifiers
     source          1..10265
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        10..389
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        392..667
                     /label=chicken beta-actin promoter
     intron          668..1676
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1600..1622
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     protein_bind    complement(1733..1766)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     intron          2300..2365
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2495..2515
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2940..3074
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2977..2996)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3031..3050
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(3116..3149)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      3158..3167
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3164..3166,3167..3169,3170..3883)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3164..3166,3167..3169,3170..3880)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3209..3230)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3470..3489)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3817..3838
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4083..4100)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4311..4330
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(4483..5100,5101..5106,5107..5142,5143..5145,5146..5181,
                     5182..5184,5185..5220,5221..5229)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=tTA-Advanced
                     /note="In the Tet-Off(R) system, tTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(5316..5336)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5734..5745)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5874..5893)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5939..5994
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5993..6012)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     rep_origin      complement(6377..6965)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6457..6476)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    7294..7341
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     7366..7385
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(7573..8361)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDQEHQGL
                     APAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALA
                     TRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7684..7703)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     8288..8307
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(8396..8756)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8410..8548
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        8565..8756
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     8713..8733
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             complement(join(8816..9607,9608..9676))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9439..9458
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9677..9781)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      9808..10263
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9895..9914)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10105..10126
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gcgcgcgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
       61 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
      121 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
      181 caatagggac tttccattga cgtcaatggg tggactattt acggtaaact gcccacttgg
      241 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
      301 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
      361 tctacgtatt agtcatcgct attaccatgg gtcgaggtga gccccacgtt ctgcttcact
      421 ctccccatct cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt
      481 tgtgcagcga tgggggcggg gggggggggg gcgcgcgcca ggcggggcgg ggcggggcga
      541 ggggcggggc ggggcgaggc ggagaggtgc ggcggcagcc aatcagagcg gcgcgctccg
      601 aaagtttcct tttatggcga ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg
      661 gcgggcggga gtcgctgcgt tgccttcgcc ccgtgccccg ctccgcgccg cctcgcgccg
      721 cccgccccgg ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc
      781 tcctccgggc tgtaattagc gcttggttta atgacggctc gtttcttttc tgtggctgcg
      841 tgaaagcctt aaagggctcc gggagggccc tttgtgcggg ggggagcggc tcggggggtg
      901 cgtgcgtgtg tgtgtgcgtg gggagcgccg cgtgcggccc gcgctgcccg gcggctgtga
      961 gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcgtg tgcgcgaggg gagcgcggcc
     1021 gggggcggtg ccccgcggtg cgggggggct gcgaggggaa caaaggctgc gtgcggggtg
     1081 tgtgcgtggg ggggtgagca gggggtgtgg gcgcggcggt cgggctgtaa cccccccctg
     1141 cacccccctc cccgagttgc tgagcacggc ccggcttcgg gtgcggggct ccgtgcgggg
     1201 cgtggcgcgg ggctcgccgt gccgggcggg gggtggcggc aggtgggggt gccgggcggg
     1261 gcggggccgc ctcgggccgg ggagggctcg ggggaggggc gcggcggccc cggagcgccg
     1321 gcggctgtcg aggcgcggcg agccgcagcc attgcctttt atggtaatcg tgcgagaggg
     1381 cgcagggact tcctttgtcc caaatctggc ggagccgaaa tctgggaggc gccgccgcac
     1441 cccctctagc gggcgcgggc gaagcggtgc ggcgccggca ggaaggaaat gggcggggag
     1501 ggccttcgtg cgtcgccgcg ccgccgtccc cttctccatc tccagcctcg gggctgccgc
     1561 agggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga
     1621 ccggcggctc tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc
     1681 tgggcaacgt gctggttgtt gtgctgtctc atcattttgg caaagaattc taataacttc
     1741 gtatagcata cattatacga agttatatta agggttccgg atcctcgggg acaccaaata
     1801 tggcgatctc ggccttttcg tttcttggag ctgggacatg tttgccatcg atccatctac
     1861 caccagaacg gccgttagat ctgctgccac cgttgtttcc accgaagaaa ccaccgttgc
     1921 cgtaaccacc acgacggttg ttgctaaaga agctgccacc gccacggcca ccgttgtagc
     1981 cgccgttgtt gttattgtag ttgctcatgt tatttctggc acttcttggt tttcctctta
     2041 agtgaggagg aacataacca ttctcgttgt tgtcgttgat gcttaaattt tgcacttgtt
     2101 cgctcagttc agccataata tgaaatgctt ttcttgttgt tcttacggaa taccacttgc
     2161 cacctatcac cacaactaac tttttcccgt tcctccatct cttttatatt ttttttctcg
     2221 agggatcttt gtgaaggaac cttacttctg tggtgtgaca taattggaca aactacctac
     2281 agagatttaa agctctaagg taaatataaa atttttaagt gtataatgtg ttaaactact
     2341 gattctaatt gtttgtgtat tttagattcc aacctatgga actgatgaat gggagcagtg
     2401 gtggaatgcc tttaatgagg aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga
     2461 tgaggctact gctgactctc aacattctac tcctccaaaa aagaagagaa aggtagaaga
     2521 ccccaaggac tttccttcag aattgctaag ttttttgagt catgctgtgt ttagtaatag
     2581 aactcttgct tgctttgcta tttacaccac aaaggaaaaa gctgcactgc tatacaagaa
     2641 aattatggaa aaatattctg taacctttat aagtaggcat aacagttata atcataacat
     2701 actgtttttt cttactccac acaggcatag agtgtctgct attaataact atgctcaaaa
     2761 attgtgtacc tttagctttt taatttgtaa aggggttaat aaggaatatt tgatgtatag
     2821 tgccttgact agagatcata atcagccata ccacatttgt agaggtttta cttgctttaa
     2881 aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta
     2941 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     3001 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     3061 atcatgtctg gatctgacat ggtaagtaag cttgggctgc aggtcgaggg acctaataac
     3121 ttcgtatagc atacattata cgaagttata tgtcgacgcc accatggtga gcaagggcga
     3181 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     3241 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     3301 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
     3361 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
     3421 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     3481 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     3541 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     3601 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
     3661 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     3721 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
     3781 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     3841 cgccgccggg atcactctcg gcatggacga gctgtacaag taagatatcc tagatcgagt
     3901 taattaagga ttccgcccct ctccctcccc cccccctaac gttactggcc gaagccgctt
     3961 ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg
     4021 caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc
     4081 ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga
     4141 agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc
     4201 tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc
     4261 acaaccccag tgccacgttg tgagttggat agttgtggaa agagtaaaaa tggctctcct
     4321 caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg
     4381 atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa aaacgtctag
     4441 gccccccgaa ccacggggac gtggtttcct ttgaaaaaca cgatgtctag actggacaag
     4501 agcaaagtca taaactctgc tctggaatta ctcaatgaag tcggtatcga aggcctgacg
     4561 acaaggaaac tcgctcaaaa gctgggagtt gagcagccta ccctgtactg gcacgtgaag
     4621 aacaagcggg ccctgctcga tgccctggca atcgagatgc tggacaggca tcatacccac
     4681 ttctgccccc tggaaggcga gtcatggcaa gactttctgc ggaacaacgc caagtcattc
     4741 cgctgtgctc tcctctcaca tcgcgacggg gctaaagtgc atctcggcac ccgcccaaca
     4801 gagaaacagt acgaaaccct ggaaaatcag ctcgcgttcc tgtgtcagca aggcttctcc
     4861 ctggagaacg cactgtacgc tctgtccgcc gtgggccact ttacactggg ctgcgtattg
     4921 gaggatcagg agcatcaagt agcaaaagag gaaagagaga cacctaccac cgattctatg
     4981 cccccacttc tgagacaagc aattgagctg ttcgaccatc agggagccga acctgccttc
     5041 cttttcggcc tggaactaat catatgtggc ctggagaaac agctaaagtg cgaaagcggc
     5101 gggccggccg acgcccttga cgattttgac ttagacatgc tcccagccga tgcccttgac
     5161 gactttgacc ttgatatgct gcctgctgac gctcttgacg attttgacct tgacatgctc
     5221 cccgggtaac taagtaagcg gccgcgaatt cgcccttcgg ccgctcaacc tctggattac
     5281 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     5341 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     5401 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     5461 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     5521 acctgtcagc tcctttccgg aactttcgct ttccccctcc ctattgccac ggcggaactc
     5581 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     5641 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctatgt tgccacctgg
     5701 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     5761 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     5821 agtcggatct ccctttgggc cgcctccccg cagcggccgc ggcgactcga ggatcttttt
     5881 ccctctgcca aaaattatgg ggacatcatg aagccccttg agcatctgac ttctggctaa
     5941 taaaggaaat ttattttcat tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga
     6001 aggacatatg ggagggcaaa tcatttaaaa catcagaatg agtatttggt ttagagtttg
     6061 gcaacatatg ccatatgctg gctgccatga acaaaggtgg ctataaagag gtcatcagta
     6121 tatgaaacag ccccctgctg tccattcctt attccataga aaagccttga cttgaggtta
     6181 gatttttttt atattttgtt ttgtgttatt tttttcttta acatccctaa aattttcctt
     6241 acatgtttta ctagccagat ttttcctcct ctcctgacta ctcccagtca tagctgtccc
     6301 tcttctctta tgaagccatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     6361 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     6421 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     6481 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     6541 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     6601 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     6661 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     6721 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     6781 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     6841 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     6901 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     6961 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     7021 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     7081 aaaaatgaag ttttaaatca atctaaagta tatatgagta acctgaggct atggcagggc
     7141 ctgccgcccc gacgttggct gcgagccctg ggccttcacc cgaacttggg gggtggggtg
     7201 gggaaaagga agaaacgcgg gcgtattggc cccaatgggg tctcggtggg gtatcgacag
     7261 agtgccagcc ctgggaccga accccgcgtt tatgaacaaa cgacccaaca ccgtgcgttt
     7321 tattctgtct ttttattgcc gtcatagcgc gggttccttc cggtattgtc tccttccgtg
     7381 tttcagttag cctcccccta gggtgggcga agaactccag catgagatcc ccgcgctgga
     7441 ggatcatcca gccggcgtcc cggaaaacga ttccgaagcc caacctttca tagaaggcgg
     7501 cggtggaatc gaaatctcgt gatggcaggt tgggcgtcgc ttggtcggtc atttcgaacc
     7561 ccagagtccc gctcagaaga actcgtcaag aaggcgatag aaggcgatgc gctgcgaatc
     7621 gggagcggcg ataccgtaaa gcacgaggaa gcggtcagcc cattcgccgc caagctcttc
     7681 agcaatatca cgggtagcca acgctatgtc ctgatagcgg tccgccacac ccagccggcc
     7741 acagtcgatg aatccagaaa agcggccatt ttccaccatg atattcggca agcaggcatc
     7801 gccatgggtc acgacgagat cctcgccgtc gggcatgctc gccttgagcc tggcgaacag
     7861 ttcggctggc gcgagcccct gatgctcttg atcatcctga tcgacaagac cggcttccat
     7921 ccgagtacgt gctcgctcga tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg
     7981 atcaagcgta tgcagccgcc gcattgcatc agccatgatg gatactttct cggcaggagc
     8041 aaggtgagat gacaggagat cctgccccgg cacttcgccc aatagcagcc agtcccttcc
     8101 cgcttcagtg acaacgtcga gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga
     8161 tagccgcgct gcctcgtctt gcagttcatt cagggcaccg gacaggtcgg tcttgacaaa
     8221 aagaaccggg cgcccctgcg ctgacagccg gaacacggcg gcatcagagc agccgattgt
     8281 ctgttgtgcc cagtcatagc cgaatagcct ctccacccaa gcggccggag aacctgcgtg
     8341 caatccatct tgttcaatca tgcgaaacga tcctcatcct gtctcttgat cgatctttgc
     8401 aaaagcctag gcctccaaaa aagcctcctc actacttctg gaatagctca gaggccgagg
     8461 aggcggcctc ggcctctgca taaataaaaa aaattagtca gccatggggc ggagaatggg
     8521 cggaactggg cggagttagg ggcgggatgg gcggagttag gggcgggact atggttgctg
     8581 actaattgag atgcatgctt tgcatacttc tgcctgctgg ggagcctggg gactttccac
     8641 acctggttgc tgactaattg agatgcatgc tttgcatact tctgcctgct ggggagcctg
     8701 gggactttcc acaccctaac tgacacacat tccacagctg gttctttccg cctcaggact
     8761 cttccttttt caataaatca atctaaagta tatatgagta aacttggtct gacagttacc
     8821 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     8881 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     8941 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     9001 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     9061 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     9121 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     9181 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     9241 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     9301 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     9361 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     9421 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     9481 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     9541 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     9601 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     9661 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     9721 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     9781 gcacatttcc ccgaaaagtg ccacctgacg cgccctgtag cggcgcatta agcgcggcgg
     9841 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     9901 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     9961 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
    10021 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
    10081 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
    10141 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
    10201 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa
    10261 tttac
//
