LOCUS       sequence-167294-       11519 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  target  miRNA406  for destruction in Arabidopsis thaliana.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11519)
  AUTHORS   Tang G, Yan J, Gu Y, Qiao M, Fan R, Mao Y, Tang X
  TITLE     Construction of short tandem target mimic (STTM) to block the 
            functions of plant and animal microRNAs.
  JOURNAL   Methods. 2012 Oct;58(2):118-25. doi: 10.1016/j.ymeth.2012.10.006. 
            Epub 2012 Oct 23.
  PUBMED    23098881
REFERENCE   2  (bases 1 to 11519)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ymeth.2012.10.006"; journalName: "Methods"; date: 
            "2012-10"; volume: "58"; issue: "2"; pages: "118-25"
COMMENT     Sequence Label: pFGC5941-PacI-ath-STTM406
FEATURES             Location/Qualifiers
     source          1..11519
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..346
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     289..309
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     promoter        complement(1245..1263)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1246..1263)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     1902..1921
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(2024..2029,2030..2046,2047..2052))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     terminator      2362..3069
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     primer_bind     complement(3094..3111)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3094..3110)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3103..3125)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    3313..3337
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4638..5267
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5696..6769
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      6835..7029
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(7265..7287)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7373..7513
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7428..7447
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7528..7545)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7699..8287)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8374..9168)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    9593..9617
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(9703..9955)
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     CDS             complement(9965..10516)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(10522..10902)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     primer_bind     11094..11113
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        11094..11112
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tgagactttt caacaaaggg taatttcggg aaacctcctc ggattccatt gcccagctat
       61 ctgtcacctc atcgaaagga cagtagaaaa ggaaggtggc tcctacaaat gccatcattg
      121 cgataaagga aaggctatca ttcaagatgc ctctgccgac agtggtccca aagatggacc
      181 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
      241 ggattgatgt gacatctcca ctgacataag ggatgacgca caaccccact atccttcgca
      301 agacccttcc tctatataag gaagttcatt tcatttggag aggacagccc aagcttctgg
      361 attacaactg tagcattcta gttgttgttg ttatggtcta atttaaatat ggtctaaaga
      421 agaagaatct ggattacaac tgtagcattc tagaattcgg tacgctgaaa tcaccagtct
      481 ctctctacaa atctatccct ctctattttc tccataaata atgtgtgagt agtttcccga
      541 taagggaaat tagggttctt atagggtctc gctcatgtgt tgagcatata agaaaccctt
      601 agtatgtatt tgtatttgta aaatacttct accaataaaa tttctaattc ctaaaaccaa
      661 aatccagtac taaaatccag atctcctaaa gtccctatag atctttgtcg tgaatataaa
      721 ccagacacga gacgactaga cctggagccc agacgccgtt cgaagctaga agtaccgctt
      781 aggcaggagg ccgttaggga aaagatgcta aggcagggtt ggttacgttg actcccccgt
      841 aggtttggct taaatatgat gaagtggacg gaaggaagga ggaagacaag gaaggataag
      901 gttgcaggcc ctgtgcaagg taagaagatg gaaatttgat agaggtacgc tactatactt
      961 atactatacg ctaagggaat gattgtattt ataccctata ccccctaata accccttatc
     1021 aatttaagaa ataatccgca taagcccccg cttaaaaatt ggtatcagag ccatgaatag
     1081 gtctatgacc aaaactcaag aggataaaac ctcaccaaaa tacaaaagag tccttaactc
     1141 taaagataaa agatctttca agatcaaaac tagttccctc acaccggtga cggggatcgc
     1201 atgcgacctc tagaccatca cattggagct tcagctgctc gagttctata gtgtcaccta
     1261 aatcgtatgt gtatgataca taaggttatg tattaattgt agccgcgttc ttacgtcgac
     1321 gtaccaatta cgccccgccc tgtcactcat cgcagtactg ttgtaattca ttaagcattc
     1381 tgccgacatg gaagccatca caaacggcat gatgaacctg aatcgccagc ggcatcagca
     1441 ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca
     1501 tattggccac gtttaaatca aaactggtga aactcaccca gggattggct gagacgaaaa
     1561 acatattctc aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat
     1621 cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg
     1681 aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatta
     1741 ccagctcacc gtctttcatt gccatacgga actccggatg agcattcatc aggcgggcaa
     1801 gaatgtgaat aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg
     1861 ccgtaatatc cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct
     1921 caaaatgctc tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttctt
     1981 tctccatttt agcttcctta gctcctgaaa atctcgataa ctcattaaag cttatcgatg
     2041 ataagctgtc aaacatgaga atttagcccg cctaatgagc gggctttttg ttgtgatccg
     2101 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     2161 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt cccccctgga
     2221 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     2281 cttaattaag tttgcaagca gcagattacc ggatacctgt ccgcctttct taattaagac
     2341 ccgggactag tccctagagt cctgctttaa tgagatatgc gagacgccta tgatcgcatg
     2401 atatttgctt tcaattctgt tgtgcacgtt gtaaaaaacc tgagcatgtg tagctcagat
     2461 ccttaccgcc ggtttcggtt cattctaatg aatatatcac ccgttactat cgtattttta
     2521 tgaataatat tctccgttca atttactgat tgtaccctac tacttatatg tacaatatta
     2581 aaatgaaaac aatatattgt gctgaatagg tttatagcga catctatgat agagcgccac
     2641 aataacaaac aattgcgttt tattattaca aatccaattt taaaaaaagc ggcagaaccg
     2701 gtcaaaccta aaagactgat tacataaatc ttattcaaat ttcaaaagtg ccccaggggc
     2761 tagtatctac gacacaccga gcggcgaact aataacgctc actgaaggga actccggttc
     2821 cccgccggcg cgcatgggtg agattccttg aagttgagta ttggccgtcc gctctaccga
     2881 aagttacggg caccattcaa cccggtccag cacggcggcc gggtaaccga cttgctgccc
     2941 cgagaattat gcagcatttt tttggtgtat gtgggcccca aatgaagtgc aggtcaaacc
     3001 ttgacagtga cgacaaatcg ttgggcgggt ccagggcgaa ttttgcgaca acatgtcgag
     3061 gctcagcagg acctgcaggc atgcaagctt ggcactggcc gtcgttttac aacgtcgtga
     3121 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
     3181 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
     3241 tggcgaatgc tagagcagct tgagcttgga tcagattgtc gtttcccgcc ttcagtttaa
     3301 actatcagtg tttgacagga tatattggcg ggtaaaccta agagaaaaga gcgtttatta
     3361 gaataatcgg atatttaaaa gggcgtgaaa aggtttatcc gttcgtccat ttgtatgtgc
     3421 atgccaacca cagggttccc ctcgggatca aagtactttg atccaacccc tccgctgcta
     3481 tagtgcagtc ggcttctgac gttcagtgca gccgtcttct gaaaacgaca tgtcgcacaa
     3541 gtcctaagtt acgcgacagg ctgccgccct gcccttttcc tggcgttttc ttgtcgcgtg
     3601 ttttagtcgc ataaagtaga atacttgcga ctagaaccgg agacattacg ccatgaacaa
     3661 gagcgccgcc gctggcctgc tgggctatgc ccgcgtcagc accgacgacc aggacttgac
     3721 caaccaacgg gccgaactgc acgcggccgg ctgcaccaag ctgttttccg agaagatcac
     3781 cggcaccagg cgcgaccgcc cggagctggc caggatgctt gaccacctac gccctggcga
     3841 cgttgtgaca gtgaccaggc tagaccgcct ggcccgcagc acccgcgacc tactggacat
     3901 tgccgagcgc atccaggagg ccggcgcggg cctgcgtagc ctggcagagc cgtgggccga
     3961 caccaccacg ccggccggcc gcatggtgtt gaccgtgttc gccggcattg ccgagttcga
     4021 gcgttcccta atcatcgacc gcacccggag cgggcgcgag gccgccaagg cccgaggcgt
     4081 gaagtttggc ccccgcccta ccctcacccc ggcacagatc gcgcacgccc gcgagctgat
     4141 cgaccaggaa ggccgcaccg tgaaagaggc ggctgcactg cttggcgtgc atcgctcgac
     4201 cctgtaccgc gcacttgagc gcagcgagga agtgacgccc accgaggcca ggcggcgcgg
     4261 tgccttccgt gaggacgcat tgaccgaggc cgacgccctg gcggccgccg agaatgaacg
     4321 ccaagaggaa caagcatgaa accgcaccag gacggccagg acgaaccgtt tttcattacc
     4381 gaagagatcg aggcggagat gatcgcggcc gggtacgtgt tcgagccgcc cgcgcacgtc
     4441 tcaaccgtgc ggctgcatga aatcctggcc ggtttgtctg atgccaagct ggcggcctgg
     4501 ccggccagct tggccgctga agaaaccgag cgccgccgtc taaaaaggtg atgtgtattt
     4561 gagtaaaaca gcttgcgtca tgcggtcgct gcgtatatga tgcgatgagt aaataaacaa
     4621 atacgcaagg ggaacgcatg aaggttatcg ctgtacttaa ccagaaaggc gggtcaggca
     4681 agacgaccat cgcaacccat ctagcccgcg ccctgcaact cgccggggcc gatgttctgt
     4741 tagtcgattc cgatccccag ggcagtgccc gcgattgggc ggccgtgcgg gaagatcaac
     4801 cgctaaccgt tgtcggcatc gaccgcccga cgattgaccg cgacgtgaag gccatcggcc
     4861 ggcgcgactt cgtagtgatc gacggagcgc cccaggcggc ggacttggct gtgtccgcga
     4921 tcaaggcagc cgacttcgtg ctgattccgg tgcagccaag cccttacgac atatgggcca
     4981 ccgccgacct ggtggagctg gttaagcagc gcattgaggt cacggatgga aggctacaag
     5041 cggcctttgt cgtgtcgcgg gcgatcaaag gcacgcgcat cggcggtgag gttgccgagg
     5101 cgctggccgg gtacgagctg cccattcttg agtcccgtat cacgcagcgc gtgagctacc
     5161 caggcactgc cgccgccggc acaaccgttc ttgaatcaga acccgagggc gacgctgccc
     5221 gcgaggtcca ggcgctggcc gctgaaatta aatcaaaact catttgagtt aatgaggtaa
     5281 agagaaaatg agcaaaagca caaacacgct aagtgccggc cgtccgagcg cacgcagcag
     5341 caaggctgca acgttggcca gcctggcaga cacgccagcc atgaagcggg tcaactttca
     5401 gttgccggcg gaggatcaca ccaagctgaa gatgtacgcg gtacgccaag gcaagaccat
     5461 taccgagctg ctatctgaat acatcgcgca gctaccagag taaatgagca aatgaataaa
     5521 tgagtagatg aattttagcg gctaaaggag gcggcatgga aaatcaagaa caaccaggca
     5581 ccgacgccgt ggaatgcccc atgtgtggag gaacgggcgg ttggccaggc gtaagcggct
     5641 gggttgcctg ccggccctgc aatggcactg gaacccccaa gcccgaggaa tcggcgtgag
     5701 cggtcgcaaa ccatccggcc cggtacaaat cggcgcggcg ctgggtgatg acctggtgga
     5761 gaagttgaag gccgcgcagg ccgcccagcg gcaacgcatc gaggcagaag cacgccccgg
     5821 tgaatcgtgg caagcggccg ctgatcgaat ccgcaaagaa tcccggcaac cgccggcagc
     5881 cggtgcgccg tcgattagga agccgcccaa gggcgacgag caaccagatt ttttcgttcc
     5941 gatgctctat gacgtgggca cccgcgatag tcgcagcatc atggacgtgg ccgttttccg
     6001 tctgtcgaag cgtgaccgac gagctggcga ggtgatccgc tacgagcttc cagacgggca
     6061 cgtagaggtt tccgcagggc cggccggcat ggccagtgtg tgggattacg acctggtact
     6121 gatggcggtt tcccatctaa ccgaatccat gaaccgatac cgggaaggga agggagacaa
     6181 gcccggccgc gtgttccgtc cacacgttgc ggacgtactc aagttctgcc ggcgagccga
     6241 tggcggaaag cagaaagacg acctggtaga aacctgcatt cggttaaaca ccacgcacgt
     6301 tgccatgcag cgtacgaaga aggccaagaa cggccgcctg gtgacggtat ccgagggtga
     6361 agccttgatt agccgctaca agatcgtaaa gagcgaaacc gggcggccgg agtacatcga
     6421 gatcgagcta gctgattgga tgtaccgcga gatcacagaa ggcaagaacc cggacgtgct
     6481 gacggttcac cccgattact ttttgatcga tcccggcatc ggccgttttc tctaccgcct
     6541 ggcacgccgc gccgcaggca aggcagaagc cagatggttg ttcaagacga tctacgaacg
     6601 cagtggcagc gccggagagt tcaagaagtt ctgtttcacc gtgcgcaagc tgatcgggtc
     6661 aaatgacctg ccggagtacg atttgaagga ggaggcgggg caggctggcc cgatcctagt
     6721 catgcgctac cgcaacctga tcgagggcga agcatccgcc ggttcctaat gtacggagca
     6781 gatgctaggg caaattgccc tagcagggga aaaaggtcga aaaggtctct ttcctgtgga
     6841 tagcacgtac attgggaacc caaagccgta cattgggaac cggaacccgt acattgggaa
     6901 cccaaagccg tacattggga accggtcaca catgtaagtg actgatataa aagagaaaaa
     6961 aggcgatttt tccgcctaaa actctttaaa acttattaaa actcttaaaa cccgcctggc
     7021 ctgtgcataa ctgtctggcc agcgcacagc cgaagagctg caaaaagcgc ctacccttcg
     7081 gtcgctgcgc tccctacgcc ccgccgcttc gcgtcggcct atcgcggccg ctggccgctc
     7141 aaaaatggct ggcctacggc caggcaatct accagggcgc ggacaagccg cgccgtcgcc
     7201 actcgaccgc cggcgcccac atcaaggcac cctgcctcgc gcgtttcggt gatgacggtg
     7261 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
     7321 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca
     7381 tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca
     7441 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
     7501 ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     7561 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     7621 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     7681 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     7741 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     7801 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     7861 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     7921 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     7981 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     8041 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     8101 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     8161 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     8221 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     8281 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     8341 acgttaaggg attttggtca tgcattctag gtactaaaac aattcatcca gtaaaatata
     8401 atattttatt ttctcccaat caggcttgat ccccagtaag tcaaaaaata gctcgacata
     8461 ctgttcttcc ccgatatcct ccctgatcga ccggacgcag aaggcaatgt cataccactt
     8521 gtccgccctg ccgcttctcc caagatcaat aaagccactt actttgccat ctttcacaaa
     8581 gatgttgctg tctcccaggt cgccgtggga aaagacaagt tcctcttcgg gcttttccgt
     8641 ctttaaaaaa tcatacagct cgcgcggatc tttaaatgga gtgtcttctt cccagttttc
     8701 gcaatccaca tcggccagat cgttattcag taagtaatcc aattcggcta agcggctgtc
     8761 taagctattc gtatagggac aatccgatat gtcgatggag tgaaagagcc tgatgcactc
     8821 cgcatacagc tcgataatct tttcagggct ttgttcatct tcatactctt ccgagcaaag
     8881 gacgccatcg gcctcactca tgagcagatt gctccagcca tcatgccgtt caaagtgcag
     8941 gacctttgga acaggcagct ttccttccag ccatagcatc atgtcctttt cccgttccac
     9001 atcataggtg gtccctttat accggctgtc cgtcattttt aaatataggt tttcattttc
     9061 tcccaccagc ttatatacct tagcaggaga cattccttcc gtatctttta cgcagcggta
     9121 tttttcgatc agttttttca attccggtga tattctcatt ttagccattt attatttcct
     9181 tcctcttttc tacagtattt aaagataccc caagaagcta attataacaa gacgaactcc
     9241 aattcactgt tccttgcatt ctaaaacctt aaataccaga aaacagcttt ttcaaagttg
     9301 ttttcaaagt tggcgtataa catagtatcg acggagccga ttttgaaacc gcggtgatca
     9361 caggcagcaa cgctctgtca tcgttacaat caacatgcta ccctccgcga gatcatccgt
     9421 gtttcaaacc cggcagctta gttgccgttc ttccgaatag catcggtaac atgagcaaag
     9481 tctgccgcct tacaacggct ctcccgctga cgccgtcccg gactgatggg ctgcctgtat
     9541 cgagtggtga ttttgtgccg agctgccggt cggggagctg ttggctggct ggtggcagga
     9601 tatattgtgg tgtaaacaaa ttgacgctta gacaacttaa taacacattg cggacgtttt
     9661 taatgtactg aattaacgcc gaattaattc gagctcggat ctgataattt atttgaaaat
     9721 tcataagaaa agcaaacgtt acatgaattg atgaaacaat acaaagacag ataaagccac
     9781 gcacatttag gatattggcc gagattactg aatattgagt aagatcacgg aatttctgac
     9841 aggagcatgt cttcaattca gcccaaatgg cagttgaaat actcaaaccg ccccatatgc
     9901 aggagcggat cattcattgt ttgtttggtt gcctttgcca acatgggagt ccaagattct
     9961 gcagtcaaat ctcggtgacg ggcaggaccg gacggggcgg taccggcagg ctgaagtcca
    10021 gctgccagaa acccacgtca tgccagttcc cgtgcttgaa gccggccgcc cgcagcatgc
    10081 cgcggggggc atatccgagc gcctcgtgca tgcgcacgct cgggtcgttg ggcagcccga
    10141 tgacagcgac cacgctcttg aagccctgtg cctccaggga cttcagcagg tgggtgtaga
    10201 gcgtggagcc cagtcccgtc cgctggtggc ggggggagac gtacacggtc gactcggccg
    10261 tccagtcgta ggcgttgcgt gccttccagg ggcccgcgta ggcgatgccg gcgacctcgc
    10321 cgtccacctc ggcgacgagc cagggatagc gctcccgcag acggacgagg tcgtccgtcc
    10381 actcctgcgg ttcctgcggc tcggtacgga agttgaccgt gcttgtctcg atgtagtggt
    10441 tgacgatggt gcagaccgcc ggcatgtccg cctcggtggc acggcggatg tcggccgggc
    10501 gtcgttctgg gctcatcgat tcgatttggt gtatcgagat tggttatgaa attcagatgc
    10561 tagtgtaatg tattggtaat ttgggaagat ataataggaa gcaaggctat ttatccattt
    10621 ctgaaaaggc gaaatggcgt caccgcgagc gtcacgcgca ttccgttctt gctgtaaagc
    10681 gttgtttggt acacttttga ctagcgaggc ttggcgtgtc agcgtatcta ttcaaaagtc
    10741 gttaatggct gcggatcaag aaaaagttgg aatagaaaca gaatacccgc gaaattcagg
    10801 cccggttgcc atgtcctaca cgccgaaata aacgaccaaa ttagtagaaa aataaaaact
    10861 gactcggata cttacgtcac gtcttgcgca ctgatttgaa aaatctcaga attcatggat
    10921 cctaggtgag tctagagagt taattaagtt tgcaagcagc agattacgcg cagaaaaaaa
    10981 ggatctcaag gagatccttt ggatcacaaa aaaaaagccc gctcattagg cgggttaaat
    11041 tcccgggtac tgcagccgat tcattaatgc aggttaacct ggcttatcga aattaatacg
    11101 actcactata gggaggccgg cagatctgat ccctactcca aaaatgtcaa agatacagtc
    11161 tcagaagacc aaagggctat tgagactttt caacaaaggg taatttcggg aaacctcctc
    11221 ggattccatt gcccagctat ctgtcacttc atcgaaagga cagtggaaaa ggaaggtggc
    11281 tcctacaaat gccatcattg cgataaagga aaggctatca ttcaggatgc ctctgccgac
    11341 agtggtccca aagatggacc cccacccacg aggagcatcg tgggaaaaga agacgttcca
    11401 accacgtctt caaagcaagt ggattgatgt gacatctcca ctgacgtaag ggatgacgca
    11461 caatcccacc cctactccaa aaatgtcaaa gatactgtct cagaagacca aagggctat
//
