LOCUS       sequence-167295-         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of miR-31.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  TITLE     Baar lab plamids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pmR-mCherry miR-31
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      25..34
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             31..738
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(176..194)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     444..463
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 tcagatccgc tagcgctacc ggtcgccacc atggtgagca agggcgagga ggataacatg
       61 gccatcatca aggagttcat gcgcttcaag gtgcacatgg agggctccgt gaacggccac
      121 gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag
      181 ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc ccctcagttc
      241 atgtacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta cttgaagctg
      301 tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg
      361 accgtgaccc aggactcctc cctgcaggac ggcgagttca tctacaaggt gaagctgcgc
      421 ggcaccaact tcccctccga cggccccgta atgcagaaga agaccatggg ctgggaggcc
      481 tcctccgagc ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa gcagaggctg
      541 aagctgaagg acggcggcca ctacgacgct gaggtcaaga ccacctacaa ggccaagaag
      601 cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt tggacatcac ctcccacaac
      661 gaggactaca ccatcgtgga acagtacgaa cgcgccgagg gccgccactc caccggcggc
      721 atggacgagc tgtacaagtc cggaaactag tctcagatct cgagctcaag cttaactgcc
      781 ttcaaaattg gacactctaa ggaccactgc attgttacat taggaaacag cttgtgtttt
//
