LOCUS       sequence-167300-         857 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For Flpe/FRT-based Supernova-RFP, pK037 should be used with pK036..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Luo W, Mizuno H, Iwata R, Nakazawa S, Yasuda K, Itohara S, Iwasato T
  TITLE     Supernova: A Versatile Vector System for Single-Cell Labeling and 
            Gene Function Studies in vivo.
  JOURNAL   Sci Rep. 2016 Oct 24;6:35747. doi: 10.1038/srep35747.
  PUBMED    27775045
REFERENCE   2  (bases 1 to 857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-10-24"; volume: "6:35747. doi"; pages: " 
            10.1038/srep35747"
COMMENT     Sequence Label: pK037.CAG-FRT-stop-FRT-RFP-ires-tTA-WPRE (Supe...
FEATURES             Location/Qualifiers
     source          1..857
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(52..85)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     CDS             128..823
                     /codon_start=1
                     /product="red fluorescent protein from Entacmaea 
                     quadricolor"
                     /label=TurboRFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
                     VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA
                     TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK
                     LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC
                     DLPSKLGHR"
ORIGIN
        1 tgtctggatc tgacatggta agtaagcttg ggctgcaggt cgagggacct agaagttcct
       61 atactttcta gagaatagga acttcatgtc gacggtaccg cgggcccggg atccaccggt
      121 cgccaccatg agcgagctga tcaaggagaa catgcacatg aagctgtaca tggagggcac
      181 cgtgaacaac caccacttca agtgcacatc cgagggcgaa ggcaagccct acgagggcac
      241 ccagaccatg aagatcaagg tggtcgaggg cggccctctc cccttcgcct tcgacatcct
      301 ggctaccagc ttcatgtacg gcagcaaagc cttcatcaac cacacccagg gcatccccga
      361 cttctttaag cagtccttcc ctgagggctt cacatgggag agaatcacca catacgaaga
      421 cgggggcgtg ctgaccgcta cccaggacac cagcttccag aacggctgca tcatctacaa
      481 cgtcaagatc aacggggtga acttcccatc caacggccct gtgatgcaga agaaaacacg
      541 cggctgggag gccaacaccg agatgctgta ccccgctgac ggcggcctga gaggccacag
      601 ccagatggcc ctgaagctcg tgggcggggg ctacctgcac tgctccttca agaccacata
      661 cagatccaag aaacccgcta agaacctcaa gatgcccggc ttccacttcg tggaccacag
      721 actggaaaga atcaaggagg ccgacaaaga gacctacgtc gagcagcacg agatggctgt
      781 ggccaagtac tgcgacctcc ctagcaaact ggggcacaga tgatgctcta gatcgagtta
      841 attaaggatt ccgcccc
//
