LOCUS       sequence-167301-       11470 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  target  miRNA408  for destruction in Arabidopsis thaliana.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11470)
  AUTHORS   Tang G, Yan J, Gu Y, Qiao M, Fan R, Mao Y, Tang X
  TITLE     Construction of short tandem target mimic (STTM) to block the 
            functions of plant and animal microRNAs.
  JOURNAL   Methods. 2012 Oct;58(2):118-25. doi: 10.1016/j.ymeth.2012.10.006. 
            Epub 2012 Oct 23.
  PUBMED    23098881
REFERENCE   2  (bases 1 to 11470)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ymeth.2012.10.006"; journalName: "Methods"; date: 
            "2012-10"; volume: "58"; issue: "2"; pages: "118-25"
COMMENT     Sequence Label: pFGC5941-PacI-ath-STTM408
FEATURES             Location/Qualifiers
     source          1..11470
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..346
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     289..309
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     promoter        complement(1243..1261)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1244..1261)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     1900..1919
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(2022..2027,2028..2044,2045..2050))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     terminator      2312..3019
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     primer_bind     complement(3044..3061)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3044..3060)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3053..3075)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    3263..3287
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4588..5217
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5646..6719
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      6785..6979
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(7215..7237)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7323..7463
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7378..7397
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7478..7495)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7649..8237)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8324..9118)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    9543..9567
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(9653..9905)
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     CDS             complement(9915..10466)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(10472..10852)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     primer_bind     11044..11063
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        11044..11062
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tgagactttt caacaaaggg taatttcggg aaacctcctc ggattccatt gcccagctat
       61 ctgtcacctc atcgaaagga cagtagaaaa ggaaggtggc tcctacaaat gccatcattg
      121 cgataaagga aaggctatca ttcaagatgc ctctgccgac agtggtccca aagatggacc
      181 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
      241 ggattgatgt gacatctcca ctgacataag ggatgacgca caaccccact atccttcgca
      301 agacccttcc tctatataag gaagttcatt tcatttggag aggacagccc aagcttgcca
      361 gggaagctaa ggcagtgcag ttgttgttgt tatggtctaa tttaaatatg gtctaaagaa
      421 gaagaatgcc agggaagact aggcagtgca tgaattcggt acgctgaaat caccagtctc
      481 tctctacaaa tctatctctc tctattttct ccataaataa tgtgtgagta gtttcccgat
      541 aagggaaatt agggttctta tagggtttcg cccatgtgtt gagcatataa gaaaccctta
      601 gtatgtattt gtatttgtaa gatacttcta tcaataaaat ttctaattcc taaaaccaaa
      661 atccagtact aaaatccaga tctcctaagt ccctatagat ctttgtcgtg aatataaacc
      721 agacacgaga cgactagacc tggagcccag acgccgttcg aagctagaag taccgcttag
      781 gcaggaggcc gttagggaaa agatgctaag gcagggttgg ttacgttgac tcccccgtag
      841 gtttggttta aatatgatga agtggacgga aggaaggagg aagacaagga aggataaggt
      901 tgcaggccct gtgcaaggta agaagatgga aatttgatag aggtacgcta ctatacttat
      961 actatacgct aagggaatga ttgtatttat accctatacc ccctaataac cccttatcaa
     1021 tttaagaaat aatccgcata agcccccgct taaaaattgg tatcagagcc atgaataggt
     1081 ctatgaccaa aactcaagag gataaaaccc caccaaaata cgaaagagtc cttaactcta
     1141 aagataaaag atctttcaag atcaaaacta gttccctcac accggtgacg gggatcgcat
     1201 gcgacctcta gaccatcaca ttggagcttc agctgctcga gttctatagt gtcacctaaa
     1261 tcgtatgtgt atgatacata aggttatgta ttaattgtag ccgcgttctt acgtcgacgt
     1321 accaattacg ccccgccctg tcactcatcg cagtactgtt gtaattcatt aagcattctg
     1381 ccgacatgga agccatcaca aacggcatga tgaacctgaa tcgccagcgg catcagcacc
     1441 ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata
     1501 ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga gacgaaaaac
     1561 atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct
     1621 tgcgaatata tgtgtagaaa ccgccggaaa tcgtcgtggt attcactcca gagcgatgaa
     1681 aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatattacc
     1741 agctcaccgt ctttcattgc catacggaac tccggatgag cattcatcag gcgggcaaga
     1801 atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc
     1861 gtaatatcca gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca
     1921 aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttctttc
     1981 tccattttag cttccttagc tcctgaaaat ctcgataact cattaaagct tatcgatgat
     2041 aagctgtcaa acatgagaat ttagcccgcc taatgagcgg gctttttgtt gtgatccgcg
     2101 ttgccggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     2161 agtcagtggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc cccctggaag
     2221 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     2281 taattaagac ccgggactag tccctagagt cctgctttaa tgagatatgc gagacgccta
     2341 tgatcgcatg atatttgctt tcaattctgt tgtgcacgtt gtaaaaaacc tgagcatgtg
     2401 tagctcagat ccttaccgcc ggtttcggtt cattctaatg aatatatcac ccgttactat
     2461 cgtattttta tgaataatat tctccgttca atttactgat tgtaccctac tacttatatg
     2521 tacaatatta aaatgaaaac aatatattgt gctgaatagg tttatagcga catctatgat
     2581 agagcgccac aataacaaac aattgcgttt tattattaca aatccaattt taaaaaaagc
     2641 ggcagaaccg gtcaaaccta aaagactgat tacataaatc ttattcaaat ttcaaaagtg
     2701 ccccaggggc tagtatctac gacacaccga gcggcgaact aataacgctc actgaaggga
     2761 actccggttc cccgccggcg cgcatgggtg agattccttg aagttgagta ttggccgtcc
     2821 gctctaccga aagttacggg caccattcaa cccggtccag cacggcggcc gggtaaccga
     2881 cttgctgccc cgagaattat gcagcatttt tttggtgtat gtgggcccca aatgaagtgc
     2941 aggtcaaacc ttgacagtga cgacaaatcg ttgggcgggt ccagggcgaa ttttgcgaca
     3001 acatgtcgag gctcagcagg acctgcaggc atgcaagctt ggcactggcc gtcgttttac
     3061 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
     3121 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     3181 gcagcctgaa tggcgaatgc tagagcagct tgagcttgga tcagattgtc gtttcccgcc
     3241 ttcagtttaa actatcagtg tttgacagga tatattggcg ggtaaaccta agagaaaaga
     3301 gcgtttatta gaataatcgg atatttaaaa gggcgtgaaa aggtttatcc gttcgtccat
     3361 ttgtatgtgc atgccaacca cagggttccc ctcgggatca aagtactttg atccaacccc
     3421 tccgctgcta tagtgcagtc ggcttctgac gttcagtgca gccgtcttct gaaaacgaca
     3481 tgtcgcacaa gtcctaagtt acgcgacagg ctgccgccct gcccttttcc tggcgttttc
     3541 ttgtcgcgtg ttttagtcgc ataaagtaga atacttgcga ctagaaccgg agacattacg
     3601 ccatgaacaa gagcgccgcc gctggcctgc tgggctatgc ccgcgtcagc accgacgacc
     3661 aggacttgac caaccaacgg gccgaactgc acgcggccgg ctgcaccaag ctgttttccg
     3721 agaagatcac cggcaccagg cgcgaccgcc cggagctggc caggatgctt gaccacctac
     3781 gccctggcga cgttgtgaca gtgaccaggc tagaccgcct ggcccgcagc acccgcgacc
     3841 tactggacat tgccgagcgc atccaggagg ccggcgcggg cctgcgtagc ctggcagagc
     3901 cgtgggccga caccaccacg ccggccggcc gcatggtgtt gaccgtgttc gccggcattg
     3961 ccgagttcga gcgttcccta atcatcgacc gcacccggag cgggcgcgag gccgccaagg
     4021 cccgaggcgt gaagtttggc ccccgcccta ccctcacccc ggcacagatc gcgcacgccc
     4081 gcgagctgat cgaccaggaa ggccgcaccg tgaaagaggc ggctgcactg cttggcgtgc
     4141 atcgctcgac cctgtaccgc gcacttgagc gcagcgagga agtgacgccc accgaggcca
     4201 ggcggcgcgg tgccttccgt gaggacgcat tgaccgaggc cgacgccctg gcggccgccg
     4261 agaatgaacg ccaagaggaa caagcatgaa accgcaccag gacggccagg acgaaccgtt
     4321 tttcattacc gaagagatcg aggcggagat gatcgcggcc gggtacgtgt tcgagccgcc
     4381 cgcgcacgtc tcaaccgtgc ggctgcatga aatcctggcc ggtttgtctg atgccaagct
     4441 ggcggcctgg ccggccagct tggccgctga agaaaccgag cgccgccgtc taaaaaggtg
     4501 atgtgtattt gagtaaaaca gcttgcgtca tgcggtcgct gcgtatatga tgcgatgagt
     4561 aaataaacaa atacgcaagg ggaacgcatg aaggttatcg ctgtacttaa ccagaaaggc
     4621 gggtcaggca agacgaccat cgcaacccat ctagcccgcg ccctgcaact cgccggggcc
     4681 gatgttctgt tagtcgattc cgatccccag ggcagtgccc gcgattgggc ggccgtgcgg
     4741 gaagatcaac cgctaaccgt tgtcggcatc gaccgcccga cgattgaccg cgacgtgaag
     4801 gccatcggcc ggcgcgactt cgtagtgatc gacggagcgc cccaggcggc ggacttggct
     4861 gtgtccgcga tcaaggcagc cgacttcgtg ctgattccgg tgcagccaag cccttacgac
     4921 atatgggcca ccgccgacct ggtggagctg gttaagcagc gcattgaggt cacggatgga
     4981 aggctacaag cggcctttgt cgtgtcgcgg gcgatcaaag gcacgcgcat cggcggtgag
     5041 gttgccgagg cgctggccgg gtacgagctg cccattcttg agtcccgtat cacgcagcgc
     5101 gtgagctacc caggcactgc cgccgccggc acaaccgttc ttgaatcaga acccgagggc
     5161 gacgctgccc gcgaggtcca ggcgctggcc gctgaaatta aatcaaaact catttgagtt
     5221 aatgaggtaa agagaaaatg agcaaaagca caaacacgct aagtgccggc cgtccgagcg
     5281 cacgcagcag caaggctgca acgttggcca gcctggcaga cacgccagcc atgaagcggg
     5341 tcaactttca gttgccggcg gaggatcaca ccaagctgaa gatgtacgcg gtacgccaag
     5401 gcaagaccat taccgagctg ctatctgaat acatcgcgca gctaccagag taaatgagca
     5461 aatgaataaa tgagtagatg aattttagcg gctaaaggag gcggcatgga aaatcaagaa
     5521 caaccaggca ccgacgccgt ggaatgcccc atgtgtggag gaacgggcgg ttggccaggc
     5581 gtaagcggct gggttgcctg ccggccctgc aatggcactg gaacccccaa gcccgaggaa
     5641 tcggcgtgag cggtcgcaaa ccatccggcc cggtacaaat cggcgcggcg ctgggtgatg
     5701 acctggtgga gaagttgaag gccgcgcagg ccgcccagcg gcaacgcatc gaggcagaag
     5761 cacgccccgg tgaatcgtgg caagcggccg ctgatcgaat ccgcaaagaa tcccggcaac
     5821 cgccggcagc cggtgcgccg tcgattagga agccgcccaa gggcgacgag caaccagatt
     5881 ttttcgttcc gatgctctat gacgtgggca cccgcgatag tcgcagcatc atggacgtgg
     5941 ccgttttccg tctgtcgaag cgtgaccgac gagctggcga ggtgatccgc tacgagcttc
     6001 cagacgggca cgtagaggtt tccgcagggc cggccggcat ggccagtgtg tgggattacg
     6061 acctggtact gatggcggtt tcccatctaa ccgaatccat gaaccgatac cgggaaggga
     6121 agggagacaa gcccggccgc gtgttccgtc cacacgttgc ggacgtactc aagttctgcc
     6181 ggcgagccga tggcggaaag cagaaagacg acctggtaga aacctgcatt cggttaaaca
     6241 ccacgcacgt tgccatgcag cgtacgaaga aggccaagaa cggccgcctg gtgacggtat
     6301 ccgagggtga agccttgatt agccgctaca agatcgtaaa gagcgaaacc gggcggccgg
     6361 agtacatcga gatcgagcta gctgattgga tgtaccgcga gatcacagaa ggcaagaacc
     6421 cggacgtgct gacggttcac cccgattact ttttgatcga tcccggcatc ggccgttttc
     6481 tctaccgcct ggcacgccgc gccgcaggca aggcagaagc cagatggttg ttcaagacga
     6541 tctacgaacg cagtggcagc gccggagagt tcaagaagtt ctgtttcacc gtgcgcaagc
     6601 tgatcgggtc aaatgacctg ccggagtacg atttgaagga ggaggcgggg caggctggcc
     6661 cgatcctagt catgcgctac cgcaacctga tcgagggcga agcatccgcc ggttcctaat
     6721 gtacggagca gatgctaggg caaattgccc tagcagggga aaaaggtcga aaaggtctct
     6781 ttcctgtgga tagcacgtac attgggaacc caaagccgta cattgggaac cggaacccgt
     6841 acattgggaa cccaaagccg tacattggga accggtcaca catgtaagtg actgatataa
     6901 aagagaaaaa aggcgatttt tccgcctaaa actctttaaa acttattaaa actcttaaaa
     6961 cccgcctggc ctgtgcataa ctgtctggcc agcgcacagc cgaagagctg caaaaagcgc
     7021 ctacccttcg gtcgctgcgc tccctacgcc ccgccgcttc gcgtcggcct atcgcggccg
     7081 ctggccgctc aaaaatggct ggcctacggc caggcaatct accagggcgc ggacaagccg
     7141 cgccgtcgcc actcgaccgc cggcgcccac atcaaggcac cctgcctcgc gcgtttcggt
     7201 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
     7261 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
     7321 ggcgcagcca tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg
     7381 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg
     7441 taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     7501 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     7561 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     7621 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     7681 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     7741 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     7801 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     7861 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     7921 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     7981 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     8041 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     8101 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     8161 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     8221 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     8281 acgaaaactc acgttaaggg attttggtca tgcattctag gtactaaaac aattcatcca
     8341 gtaaaatata atattttatt ttctcccaat caggcttgat ccccagtaag tcaaaaaata
     8401 gctcgacata ctgttcttcc ccgatatcct ccctgatcga ccggacgcag aaggcaatgt
     8461 cataccactt gtccgccctg ccgcttctcc caagatcaat aaagccactt actttgccat
     8521 ctttcacaaa gatgttgctg tctcccaggt cgccgtggga aaagacaagt tcctcttcgg
     8581 gcttttccgt ctttaaaaaa tcatacagct cgcgcggatc tttaaatgga gtgtcttctt
     8641 cccagttttc gcaatccaca tcggccagat cgttattcag taagtaatcc aattcggcta
     8701 agcggctgtc taagctattc gtatagggac aatccgatat gtcgatggag tgaaagagcc
     8761 tgatgcactc cgcatacagc tcgataatct tttcagggct ttgttcatct tcatactctt
     8821 ccgagcaaag gacgccatcg gcctcactca tgagcagatt gctccagcca tcatgccgtt
     8881 caaagtgcag gacctttgga acaggcagct ttccttccag ccatagcatc atgtcctttt
     8941 cccgttccac atcataggtg gtccctttat accggctgtc cgtcattttt aaatataggt
     9001 tttcattttc tcccaccagc ttatatacct tagcaggaga cattccttcc gtatctttta
     9061 cgcagcggta tttttcgatc agttttttca attccggtga tattctcatt ttagccattt
     9121 attatttcct tcctcttttc tacagtattt aaagataccc caagaagcta attataacaa
     9181 gacgaactcc aattcactgt tccttgcatt ctaaaacctt aaataccaga aaacagcttt
     9241 ttcaaagttg ttttcaaagt tggcgtataa catagtatcg acggagccga ttttgaaacc
     9301 gcggtgatca caggcagcaa cgctctgtca tcgttacaat caacatgcta ccctccgcga
     9361 gatcatccgt gtttcaaacc cggcagctta gttgccgttc ttccgaatag catcggtaac
     9421 atgagcaaag tctgccgcct tacaacggct ctcccgctga cgccgtcccg gactgatggg
     9481 ctgcctgtat cgagtggtga ttttgtgccg agctgccggt cggggagctg ttggctggct
     9541 ggtggcagga tatattgtgg tgtaaacaaa ttgacgctta gacaacttaa taacacattg
     9601 cggacgtttt taatgtactg aattaacgcc gaattaattc gagctcggat ctgataattt
     9661 atttgaaaat tcataagaaa agcaaacgtt acatgaattg atgaaacaat acaaagacag
     9721 ataaagccac gcacatttag gatattggcc gagattactg aatattgagt aagatcacgg
     9781 aatttctgac aggagcatgt cttcaattca gcccaaatgg cagttgaaat actcaaaccg
     9841 ccccatatgc aggagcggat cattcattgt ttgtttggtt gcctttgcca acatgggagt
     9901 ccaagattct gcagtcaaat ctcggtgacg ggcaggaccg gacggggcgg taccggcagg
     9961 ctgaagtcca gctgccagaa acccacgtca tgccagttcc cgtgcttgaa gccggccgcc
    10021 cgcagcatgc cgcggggggc atatccgagc gcctcgtgca tgcgcacgct cgggtcgttg
    10081 ggcagcccga tgacagcgac cacgctcttg aagccctgtg cctccaggga cttcagcagg
    10141 tgggtgtaga gcgtggagcc cagtcccgtc cgctggtggc ggggggagac gtacacggtc
    10201 gactcggccg tccagtcgta ggcgttgcgt gccttccagg ggcccgcgta ggcgatgccg
    10261 gcgacctcgc cgtccacctc ggcgacgagc cagggatagc gctcccgcag acggacgagg
    10321 tcgtccgtcc actcctgcgg ttcctgcggc tcggtacgga agttgaccgt gcttgtctcg
    10381 atgtagtggt tgacgatggt gcagaccgcc ggcatgtccg cctcggtggc acggcggatg
    10441 tcggccgggc gtcgttctgg gctcatcgat tcgatttggt gtatcgagat tggttatgaa
    10501 attcagatgc tagtgtaatg tattggtaat ttgggaagat ataataggaa gcaaggctat
    10561 ttatccattt ctgaaaaggc gaaatggcgt caccgcgagc gtcacgcgca ttccgttctt
    10621 gctgtaaagc gttgtttggt acacttttga ctagcgaggc ttggcgtgtc agcgtatcta
    10681 ttcaaaagtc gttaatggct gcggatcaag aaaaagttgg aatagaaaca gaatacccgc
    10741 gaaattcagg cccggttgcc atgtcctaca cgccgaaata aacgaccaaa ttagtagaaa
    10801 aataaaaact gactcggata cttacgtcac gtcttgcgca ctgatttgaa aaatctcaga
    10861 attcatggat cctaggtgag tctagagagt taattaagtt tgcaagcagc agattacgcg
    10921 cagaaaaaaa ggatctcaag gagatccttt ggatcacaaa aaaaaagccc gctcattagg
    10981 cgggttaaat tcccgggtac tgcagccgat tcattaatgc aggttaacct ggcttatcga
    11041 aattaatacg actcactata gggagaccgg cagatctgat ccctacgcca aaaatgtcaa
    11101 agatacagtc tcagaagacc aaagggctat tgagactttt caacaaaggg taatttcggg
    11161 aaacctcctc ggattccatt gcccagctat ctgtcacttc atcgaaagga cagtggaaaa
    11221 ggaaggtggc ccctacaaat gccatcattg cgataaagga aaggctatca ttcaagatgc
    11281 ctctgccgac agtggtccca aagatggacc cccacccacg aggagcatcg tgggaaaaga
    11341 agacgttcca accacgtctt caaagcaagt ggattgatgt gacatctcca ctgacgtaag
    11401 ggatgacgca caaatcccac ccctactcca aaaatgtcaa agatactgtc tcagaagacc
    11461 aaagggctat
//
