LOCUS       sequence-167305-       11317 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  target  miRNA415  for destruction in Arabidopsis thaliana.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11317)
  AUTHORS   Tang G, Yan J, Gu Y, Qiao M, Fan R, Mao Y, Tang X
  TITLE     Construction of short tandem target mimic (STTM) to block the 
            functions of plant and animal microRNAs.
  JOURNAL   Methods. 2012 Oct;58(2):118-25. doi: 10.1016/j.ymeth.2012.10.006. 
            Epub 2012 Oct 23.
  PUBMED    23098881
REFERENCE   2  (bases 1 to 11317)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ymeth.2012.10.006"; journalName: "Methods"; date: 
            "2012-10"; volume: "58"; issue: "2"; pages: "118-25"
COMMENT     Sequence Label: pFGC5941-PacI-ath-STTM415
FEATURES             Location/Qualifiers
     source          1..11317
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..346
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     289..309
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     polyA_signal    507..683
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(1245..1263)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1246..1263)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     1902..1921
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(2024..2029,2030..2046,2047..2052))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     terminator      2314..3021
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     primer_bind     complement(3046..3063)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3046..3062)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3055..3077)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    3265..3289
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4590..5219
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5648..6721
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      6787..6981
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(7217..7239)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7325..7465
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7380..7399
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7480..7497)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7651..8239)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8326..9120)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    9545..9569
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(9655..9907)
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     CDS             complement(9917..10468)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(10474..10854)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     primer_bind     11231..11250
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        11231..11249
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tgagactttt caacaaaggg taatttcggg aaacctcctc ggattccatt gcccagctat
       61 ctgtcacttc atcgaaagga cagtggaaaa ggaaggtggc tcctacaaat gccatcattg
      121 cgataaagga aaggctatca ttcaagatgc ctctgccgac agtggtccca aagatggacc
      181 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
      241 ggattgatgt gacatctcca ctgacataag ggatgacgca caaccccact atccttcgca
      301 agacccttcc tctatataag gaagttcatt tcatttggag aggacagccc aagcttatgt
      361 tctgtttcga ctgctctgtt gttgttgttg ttatggtcta atttaaatat ggtctaaaga
      421 agaagaatat gttctgtttc gactgctctg ttgaattcgg tacgctgaaa tcaccagtct
      481 ctctctacaa atctatctct ctctattttc tccataaata atgtgtgagt agtttcccga
      541 taagggaaat tagggttctt atagggtttc gctcatgtgt tgagcatata agaaaccctt
      601 agtatgtatt tgtatttgta aaatacttct atcaataaaa tttctaattc ctaaaaccaa
      661 aatccagtac taaaatccag atctcctaaa gtccctatag atctttgtcg tgaatataaa
      721 ccagacacga gacgactaga cctggagccc agacgccgtt cgaagctaga agtaccgctt
      781 aggcaggagg ccgttaggga aaagatgcta aggcagggtt ggttacgttg actcccccgt
      841 aggtttggtt taaatatgat gaagtggacg gaaggaagga ggaagacaag gaaggataag
      901 gttgcaggcc ctgtgcaagg taagaagatg gaaatttgat agaggtacgc tactatactt
      961 atactatacg ctaagggaat gattgtattt ataccctata ccccctaata accccttatc
     1021 aatttaagaa ataatccgca taagcccccg cttaaaaatt ggtatcagag ccatgagtag
     1081 gtctatgacc aaaactcaag aggataaaac ctcaccaaaa tacgaaagag tccttaactc
     1141 taaagataaa agatctttca agatcaaaac tagttccctc acaccggtga cggggatcgc
     1201 atgcgacctc tagaccatca cattggagct tcagctgctc gagttctata gtgtcaccta
     1261 aatcgtatgt gtatgataca taaggttatg tattaattgt agccgcgttc ttacgtcgac
     1321 gtaccaatta cgccccgccc tgtcactcat cgcagtactg ttgtaattca ttaagcattc
     1381 tgccgacatg gaagccatca caaacggcat gatgaacctg aatcgccagc ggcatcagca
     1441 ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca
     1501 tattggccac gtttaaatca aaaccggtga aactcaccca gggattggct gagacgaaaa
     1561 acatattctc aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat
     1621 cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg
     1681 aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatta
     1741 ccagctcacc gtctttcatt gccatacgga actccggatg agcattcatc aggcgggcaa
     1801 gaatgtgaat aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg
     1861 ccgtaatatc cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct
     1921 caaaatgttc tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttctt
     1981 tctccatttt agcttcctta gctcctgaaa atctcgataa ctcattaaag cttatcgatg
     2041 ataagctgtc aaacatgaga atttagcccg cctaatgagc gggctttttg ttgtgatccg
     2101 cgttgctggc gtttttccat aggctccgcc cccccgacga gcatcacaaa aatcgacgct
     2161 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt cccccctgga
     2221 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     2281 cttaattaag acccgggact agtccctaga gtcctgcttt aatgagatat gcgagacgcc
     2341 tatgatcgca tgatatttgc tttcaattct gttgtgcacg ttgtaaaaaa cctgagcatg
     2401 tgtagctcag atccttaccg ccggtttcgg ttcattctaa tgaatatatc acccgttact
     2461 atcgtatttt tatgaataat attctccgtt caatttactg attgtaccct actacttata
     2521 tgtacaatat taaaatgaaa acaatatatt gtgctgaata ggtttatagc gacatctatg
     2581 atagagcgcc acaataacaa acaattgcgt tttattatta caaatccaat tttaaaaaaa
     2641 gcggcagaac cggtcaaacc taaaagactg attacataaa tcttattcaa atttcaaaag
     2701 tgccccaggg gctagtatct acgacacacc gagcggcgaa ctaataacgc tcactgaagg
     2761 gaactccggt tccccgccgg cgcgcatggg tgagattcct tgaagttgag tattggccgt
     2821 ccgctctacc gaaagttacg ggcaccattc aacccggtcc agcacggcgg ccgggtaacc
     2881 gacttgctgc cccgagaatt atgcagcatt tttttggtgt atgtgggccc caaatgaagt
     2941 gcaggtcaaa ccttgacagt gacgacaaat cgttgggcgg gtccagggcg aattttgcga
     3001 caacatgtcg aggctcagca ggacctgcag gcatgcaagc ttggcactgg ccgtcgtttt
     3061 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
     3121 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
     3181 gcgcagcctg aatggcgaat gctagagcag cttgagcttg gatcagattg tcgtttcccg
     3241 ccttcagttt aaactatcag tgtttgacag gatatattgg cgggtaaacc taagagaaaa
     3301 gagcgtttat tagaataatc ggatatttaa aagggcgtga aaaggtttat ccgttcgtcc
     3361 atttgtatgt gcatgccaac cacagggttc ccctcgggat caaagtactt tgatccaacc
     3421 cctccgctgc tatagtgcag tcggcttctg acgttcagtg cagccgtctt ctgaaaacga
     3481 catgtcgcac aagtcctaag ttacgcgaca ggctgccgcc ctgccctttt cctggcgttt
     3541 tcttgtcgcg tgttttagtc gcataaagta gaatacttgc gactagaacc ggagacatta
     3601 cgccatgaac aagagcgccg ccgctggcct gctgggctat gcccgcgtca gcaccgacga
     3661 ccaggacttg accaaccaac gggccgaact gcacgcggcc ggctgcacca agctgttttc
     3721 cgagaagatc accggcacca ggcgcgaccg cccggagctg gccaggatgc ttgaccacct
     3781 acgccctggc gacgttgtga cagtgaccag gctagaccgc ctggcccgca gcacccgcga
     3841 cctactggac attgccgagc gcatccagga ggccggcgcg ggcctgcgta gcctggcaga
     3901 gccgtgggcc gacaccacca cgccggccgg ccgcatggtg ttgaccgtgt tcgccggcat
     3961 tgccgagttc gagcgttccc taatcatcga ccgcacccgg agcgggcgcg aggccgccaa
     4021 ggcccgaggc gtgaagtttg gcccccgccc taccctcacc ccggcacaga tcgcgcacgc
     4081 ccgcgagctg atcgaccagg aaggccgcac cgtgaaagag gcggctgcac tgcttggcgt
     4141 gcatcgctcg accctgtacc gcgcacttga gcgcagcgag gaagtgacgc ccaccgaggc
     4201 caggcggcgc ggtgccttcc gtgaggacgc attgaccgag gccgacgccc tggcggccgc
     4261 cgagaatgaa cgccaagagg aacaagcatg aaaccgcacc aggacggcca ggacgaaccg
     4321 tttttcatta ccgaagagat cgaggcggag atgatcgcgg ccgggtacgt gttcgagccg
     4381 cccgcgcacg tctcaaccgt gcggctgcat gaaatcctgg ccggtttgtc tgatgccaag
     4441 ctggcggcct ggccggccag cttggccgct gaagaaaccg agcgccgccg tctaaaaagg
     4501 tgatgtgtat ttgagtaaaa cagcttgcgt catgcggtcg ctgcgtatat gatgcgatga
     4561 gtaaataaac aaatacgcaa ggggaacgca tgaaggttat cgctgtactt aaccagaaag
     4621 gcgggtcagg caagacgacc atcgcaaccc atctagcccg cgccctgcaa ctcgccgggg
     4681 ccgatgttct gttagtcgat tccgatcccc agggcagtgc ccgcgattgg gcggccgtgc
     4741 gggaagatca accgctaacc gttgtcggca tcgaccgccc gacgattgac cgcgacgtga
     4801 aggccatcgg ccggcgcgac ttcgtagtga tcgacggagc gccccaggcg gcggacttgg
     4861 ctgtgtccgc gatcaaggca gccgacttcg tgctgattcc ggtgcagcca agcccttacg
     4921 acatatgggc caccgccgac ctggtggagc tggttaagca gcgcattgag gtcacggatg
     4981 gaaggctaca agcggccttt gtcgtgtcgc gggcgatcaa aggcacgcgc atcggcggtg
     5041 aggttgccga ggcgctggcc gggtacgagc tgcccattct tgagtcccgt atcacgcagc
     5101 gcgtgagcta cccaggcact gccgccgccg gcacaaccgt tcttgaatca gaacccgagg
     5161 gcgacgctgc ccgcgaggtc caggcgctgg ccgctgaaat taaatcaaaa ctcatttgag
     5221 ttaatgaggt aaagagaaaa tgagcaaaag cacaaacacg ctaagtgccg gccgtccgag
     5281 cgcacgcagc agcaaggctg caacgttggc cagcctggca gacacgccag ccatgaagcg
     5341 ggtcaacttt cagttgccgg cggaggatca caccaagctg aagatgtacg cggtacgcca
     5401 aggcaagacc attaccgagc tgctatctga atacatcgcg cagctaccag agtaaatgag
     5461 caaatgaata aatgagtaga tgaattttag cggctaaagg aggcggcatg gaaaatcaag
     5521 aacaaccagg caccgacgcc gtggaatgcc ccatgtgtgg aggaacgggc ggttggccag
     5581 gcgtaagcgg ctgggttgcc tgccggccct gcaatggcac tggaaccccc aagcccgagg
     5641 aatcggcgtg agcggtcgca aaccatccgg cccggtacaa atcggcgcgg cgctgggtga
     5701 tgacctggtg gagaagttga aggccgcgca ggccgcccag cggcaacgca tcgaggcaga
     5761 agcacgcccc ggtgaatcgt ggcaagcggc cgctgatcga atccgcaaag aatcccggca
     5821 accgccggca gccggtgcgc cgtcgattag gaagccgccc aagggcgacg agcaaccaga
     5881 ttttttcgtt ccgatgctct atgacgtggg cacccgcgat agtcgcagca tcatggacgt
     5941 ggccgttttc cgtctgtcga agcgtgaccg acgagctggc gaggtgatcc gctacgagct
     6001 tccagacggg cacgtagagg tttccgcagg gccggccggc atggccagtg tgtgggatta
     6061 cgacctggta ctgatggcgg tttcccatct aaccgaatcc atgaaccgat accgggaagg
     6121 gaagggagac aagcccggcc gcgtgttccg tccacacgtt gcggacgtac tcaagttctg
     6181 ccggcgagcc gatggcggaa agcagaaaga cgacctggta gaaacctgca ttcggttaaa
     6241 caccacgcac gttgccatgc agcgtacgaa gaaggccaag aacggccgcc tggtgacggt
     6301 atccgagggt gaagccttga ttagccgcta caagatcgta aagagcgaaa ccgggcggcc
     6361 ggagtacatc gagatcgagc tagctgattg gatgtaccgc gagatcacag aaggcaagaa
     6421 cccggacgtg ctgacggttc accccgatta ctttttgatc gatcccggca tcggccgttt
     6481 tctctaccgc ctggcacgcc gcgccgcagg caaggcagaa gccagatggt tgttcaagac
     6541 gatctacgaa cgcagtggca gcgccggaga gttcaagaag ttctgtttca ccgtgcgcaa
     6601 gctgatcggg tcaaatgacc tgccggagta cgatttgaag gaggaggcgg ggcaggctgg
     6661 cccgatccta gtcatgcgct accgcaacct gatcgagggc gaagcatccg ccggttccta
     6721 atgtacggag cagatgctag ggcaaattgc cctagcaggg gaaaaaggtc gaaaaggtct
     6781 ctttcctgtg gatagcacgt acattgggaa cccaaagccg tacattggga accggaaccc
     6841 gtacattggg aacccaaagc cgtacattgg gaaccggtca cacatgtaag tgactgatat
     6901 aaaagagaaa aaaggcgatt tttccgccta aaactcttta aaacttatta aaactcttaa
     6961 aacccgcctg gcctgtgcat aactgtctgg ccagcgcaca gccgaagagc tgcaaaaagc
     7021 gcctaccctt cggtcgctgc gctccctacg ccccgccgct tcgcgtcggc ctatcgcggc
     7081 cgctggccgc tcaaaaatgg ctggcctacg gccaggcaat ctaccagggc gcggacaagc
     7141 cgcgccgtcg ccactcgacc gccggcgccc acatcaaggc accctgcctc gcgcgtttcg
     7201 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt
     7261 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
     7321 ggggcgcagc catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc
     7381 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg
     7441 cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg
     7501 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     7561 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     7621 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     7681 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     7741 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     7801 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     7861 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     7921 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     7981 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     8041 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
     8101 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     8161 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     8221 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     8281 gaacgaaaac tcacgttaag ggattttggt catgcattct aggtactaaa acaattcatc
     8341 cagtaaaata taatatttta ttttctccca atcaggcttg atccccagta agtcaaaaaa
     8401 tagctcgaca tactgttctt ccccgatatc ctccctgatc gaccggacgc agaaggcaat
     8461 gtcataccac ttgtccgccc tgccgcttct cccaagatca ataaagccac ttactttgcc
     8521 atctttcaca aagatgttgc tgtctcccag gtcgccgtgg gaaaagacaa gttcctcttc
     8581 gggcttttcc gtctttaaaa aatcatacag ctcgcgcgga tctttaaatg gagtgtcttc
     8641 ttcccagttt tcgcaatcca catcggccag atcgttattc agtaagtaat ccaattcggc
     8701 taagcggctg tctaagctat tcgtataggg acaatccgat atgtcgatgg agtgaaagag
     8761 cctgatgcac tccgcataca gctcgataat cttttcaggg ctttgttcat cttcatactc
     8821 ttccgagcaa aggacgccat cggcctcact catgagcaga ttgctccagc catcatgccg
     8881 ttcaaagtgc aggacctttg gaacaggcag ctttccttcc agccatagca tcatgtcctt
     8941 ttcccgttcc acatcatagg tggtcccttt ataccggctg tccgtcattt ttaaatatag
     9001 gttttcattt tctcccacca gcttatatac cttagcagga gacattcctt ccgtatcttt
     9061 tacgcagcgg tatttttcga tcagtttttt caattccggt gatattctca ttttagccat
     9121 ttattatttc cttcctcttt tctacagtat ttaaagatac cccaagaagc taattataac
     9181 aagacgaact ccaattcact gttccttgca ttctaaaacc ttaaatacca gaaaacagct
     9241 ttttcaaagt tgttttcaaa gttggcgtat aacatagtat cgacggagcc gattttgaaa
     9301 ccgcggtgat cacaggcagc aacgctctgt catcgttaca atcaacatgc taccctccgc
     9361 gagatcatcc gtgtttcaaa cccggcagct tagttgccgt tcttccgaat agcatcggta
     9421 acatgagcaa agtctgccgc cttacaacgg ctctcccgct gacgccgtcc cggactgatg
     9481 ggctgcctgt atcgagtggt gattttgtgc cgagctgccg gtcggggagc tgttggctgg
     9541 ctggtggcag gatatattgt ggtgtaaaca aattgacgct tagacaactt aataacacat
     9601 tgcggacgtt tttaatgtac tgaattaacg ccgaattaat tcgagctcgg atctgataat
     9661 ttatttgaaa attcataaga aaagcaaacg ttacatgaat tgatgaaaca atacaaagac
     9721 agataaagcc acgcacattt aggatattgg ccgagattac tgaatattga gtaagatcac
     9781 ggaatttctg acaggagcat gtcttcaatt cagcccaaat ggcagttgaa atactcaaac
     9841 cgccccatat gcaggagcgg atcattcatt gtttgtttgg ttgcctttgc caacatggga
     9901 gtccaagatt ctgcagtcaa atctcggtga cgggcaggac cggacggggc ggtaccggca
     9961 ggctgaagtc cagctgccag aaacccacgt catgccagtt cccgtgcttg aagccggccg
    10021 cccgcagcat gccgcggggg gcatatccga gcgcctcgtg catgcgcacg ctcgggtcgt
    10081 tgggcagccc gatgacagcg accacgctct tgaagccctg tgcctccagg gacttcagca
    10141 ggtgggtgta gagcgtggag cccagtcccg tccgctggtg gcggggggag acgtacacgg
    10201 tcgactcggc cgtccagtcg taggcgttgc gtgccttcca ggggcccgcg taggcgatgc
    10261 cggcgacctc gccgtccacc tcggcgacga gccagggata gcgctcccgc agacggacga
    10321 ggtcgtccgt ccactcctgc ggttcctgcg gctcggtacg gaagttgacc gtgcttgtct
    10381 cgatgtagtg gttgacgatg gtgcagaccg ccggcatgtc cgcctcggtg gcacggcgga
    10441 tgtcggccgg gcgtcgttct gggctcatcg attcgatttg gtgtatcgag attggttatg
    10501 aaattcagat gctagtgtaa tgtattggta atttgggaag atataatagg aagcaaggct
    10561 atttatccat ttctgaaaag gcgaaatggc gtcaccgcga gcgtcacgcg cattccgttc
    10621 ttgctgtaaa gcgttgtttg gtacactttt gactagcgag gcttggcgtg tcagcgtatc
    10681 tattcaaaag tcgttaatgg ctgcggatca agaaaaagtt ggaatagaaa cagaataccc
    10741 gcgaaattca ggcccggttg ccatgtccta cacgccgaaa taaacgacca aattagtaga
    10801 aaaataaaaa ctgactcgga tacttacgtc acgtcttgcg cactgatttg aaaaatctca
    10861 gaattcatgg atcctaggtg agtctagaga gttaattaag tttgcaagca gcagattacg
    10921 cgctttgtcg agatagatgg cgtagttatg cgcaccgtga gatttcagca ctgcttccag
    10981 ttctggccag atgggattat gccgacgctg atactcttcg tgggcgtcgg ggtttacctg
    11041 catgacaaag gcttaccgga tacctgtccg cctttcttaa ttaagtttgc aagcagcaga
    11101 ttacgcgcag aaaaaaagga tctcaaggag atcctttgga tcacaaaaaa aaagcccgct
    11161 cattaggcgg gttaaattcc cgggtactgc agccgattca ttaatgcagg ttaacctggc
    11221 ttatcgaaat taatacgact cactataggg agaccggcag atctgatccc tactccaaaa
    11281 atgtcaaaga tacagtctca gaagaccaaa gggctat
//
