LOCUS       sequence-167311-       11332 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  target  miRNA472  for destruction in Arabidopsis thaliana.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11332)
  AUTHORS   Tang G, Yan J, Gu Y, Qiao M, Fan R, Mao Y, Tang X
  TITLE     Construction of short tandem target mimic (STTM) to block the 
            functions of plant and animal microRNAs.
  JOURNAL   Methods. 2012 Oct;58(2):118-25. doi: 10.1016/j.ymeth.2012.10.006. 
            Epub 2012 Oct 23.
  PUBMED    23098881
REFERENCE   2  (bases 1 to 11332)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ymeth.2012.10.006"; journalName: "Methods"; date: 
            "2012-10"; volume: "58"; issue: "2"; pages: "118-25"
COMMENT     Sequence Label: pFGC5941-PacI-ath-STTM472
FEATURES             Location/Qualifiers
     source          1..11332
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..346
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     289..309
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     promoter        complement(1398..1416)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1399..1416)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     2055..2074
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(2177..2182,2183..2199,2200..2205))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     promoter        2480..2860
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     CDS             2866..3417
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     terminator      3427..3679
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     misc_feature    3765..3789
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             4214..5008
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      5095..5683
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5837..5854
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    5869..6009
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(5935..5954)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6095..6117
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      6353..6547
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(6613..7686)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(8115..8744)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    10045..10069
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     10257..10279
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10271..10288
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10272..10288
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(10313..11020)
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     primer_bind     11246..11265
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        11246..11264
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tgagactttt caacaaaggg taatttcggg aaacctcctc ggattccatt gcccagctat
       61 ctgtcacttc atcgaaagga cagtggaaaa ggaaggtggc tcctacaaat gccatcattg
      121 cgataaagga aaggctatca ttcaagatgc ctctgccgac agtggtccca aagatggacc
      181 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
      241 ggattgatgt gacatctcca ctgacataag ggatgacgca caaccccact atccttcgca
      301 agacccttcc tctatataag gaagttcatt tcatttggag aggacagccc aagcttggta
      361 tgggcggact ggtaggaaaa agttgttgtt gttatggtct aatttcaaat atggtctaaa
      421 gaagaagaat ggtatgggcg gactggtagg aaaaagaatt cggtacgctg aaatcaccag
      481 gccatttgga gaggacagcc caagcttggt atgggcggac tggtaggaaa aagttgttgt
      541 tgttatggtc taatttaaat atggtctaaa gaagaagaat ggtatgggcg gactggtagg
      601 aaaaagaatt cggtacgctg aaatcaccag tctctctcta caaatctatc tctctctatt
      661 ttctccataa ataatgtgtg agtagtttcc cgataaggga aattagggtt cttatagggt
      721 ctcgctcatg tgttgagcat ataagaaacc cttagtatgt atttgtattt gcaaaatact
      781 tctatcaata aaatttctaa ttcctaaaac caaaatccag tactaagatc cagatctcct
      841 aaagtcccta tagatctttg tcgtgaatat aaaccagaca cgagacgact agacctggag
      901 cccagacgcc gttcgaagct agaagtaccg cttaggcagg aggccgttag ggaaaagatg
      961 ctaaggcagg gttggttacg ttgactcccc cgtaggtttg gtttaaatat gatgaagtgg
     1021 acggaaggaa ggaggaagac aaggaaggat aaggttgcag gccctgtgca aggtaagaag
     1081 atggaaattt gatagaggta cgctattata cttatactat acgctaaggg aatgattgta
     1141 tttataccct atacccccta ataacccctt atcaatttaa gaaataatcc gcataagccc
     1201 ccgcttaaaa attggtatca gagccatgaa taggtctatg accaaaactc aagaggataa
     1261 aacctcacca aaatacggaa gagtccttaa ctctaaagat aaaagatctt tcaagatcaa
     1321 aactagttcc ctcacaccgg tgacggggat cgcatgcgac ctctagacca tcacattgga
     1381 gcttcagctg ctcgagttct atagtgtcac ctaaatcgta tgtgtatgat atataaggtt
     1441 atgtattaat tgtagccgcg ttcttacgtc gacgtaccaa ttacgccccg ccctgtcact
     1501 catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacaaacgg
     1561 catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc
     1621 ccatggtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg
     1681 tgaaactcac ccagggattg gctgagacga aaaacatatt ctcaataaac cctttaggga
     1741 aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc
     1801 ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa
     1861 cggtgtaaca agggtgaaca ctatcccata ttaccagctc accgtctttc attgccatac
     1921 ggaactccgg atgagcattc atcaggcggg caagaatgtg aataagggcc ggataaaact
     1981 tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggc
     2041 tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg
     2101 atatatcaac ggtggtatat ccagtgattt ctttctccat tttagcttcc ttagctcctg
     2161 aaaatctcga taactcatta aagcttatcg atgataagct gtcaaacatg agaatttagc
     2221 ccgcctaatg agcgggcttt ttgttgtgat ccgcgttgct ggcgtttttc cataggctcc
     2281 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     2341 gactataaag ataccaggcg tttcccccct ggaagctccc tcgtgcgctc tcctgttccg
     2401 accctgccgc ttaccggata cctgtccgcc tttcttaatt aactctctag actcacctag
     2461 gatccatgaa ttctgagatt tttcaaatca gtgcgcaaga cgtgacgtaa gtatccgagt
     2521 cagtttttat ttttctacta atttggtcgt ttatttcggc gtgtaggaca tggcaaccgg
     2581 gcctgaattt cgcgggtatt ctgtttctat tccaactttt tcttgatccg cagccattaa
     2641 cgacttttga atagatacgc tgacacgcca agcctcgcta gtcaaaagtg taccaaacaa
     2701 cgctttacag caagaacgga atgcgcgtga cgctcgcggt gacgccattt cgccttttca
     2761 gaaatggata aatagccttg cttcctatta tatcttccca aattaccaat acattacact
     2821 agcatctgaa tttcataacc aatctcgata caccaaatcg aatcgatgag cccagaacga
     2881 cgcccggccg acatccgccg tgccaccgag gcggacatgc cggcggtctg caccatcgtc
     2941 aaccactaca tcgagacaag cacggtcaac ttccgtaccg agccgcagga accgcaggag
     3001 tggacggacg acctcgtccg tctgcgggag cgctatccct ggctcgtcgc cgaggtggac
     3061 ggcgaggtcg ccggcatcgc ctacgcgggc ccctggaagg cacgcaacgc ctacgactgg
     3121 acggccgagt cgaccgtgta cgtctccccc cgccaccagc ggacgggact gggctccacg
     3181 ctctacaccc acctgctgaa gtccctggag gcacagggct tcaagagcgt ggtcgctgtc
     3241 atcgggctgc ccaacgaccc gagcgtgcgc atgcacgagg cgctcggata tgccccccgc
     3301 ggcatgctgc gggcggccgg cttcaagcac gggaactggc atgacgtggg tttctggcag
     3361 ctggacttca gcctgccggt accgccccgt ccggtcctgc ccgtcaccga gatttgactg
     3421 cagaatcttg gactcccatg ttggcaaagg caaccaaaca aacaatgaat gatccgctcc
     3481 tgcatatggg gcggtttgag tatttcaact gccatttggg ctgaattgaa gacatgctcc
     3541 tgtcagaaat tccgtgatct tactcaatat tcagtaatct cggccaatat cctaaatgtg
     3601 cgtggcttta tctgtctttg tattgtttca tcaattcatg taacgtttgc ttttcttatg
     3661 aattttcaaa taaattatca gatccgagct cgaattaatt cggcgttaat tcagtacatt
     3721 aaaaacgtcc gcaatgtgtt attaagttgt ctaagcgtca atttgtttac accacaatat
     3781 atcctgccac cagccagcca acagctcccc gaccggcagc tcggcacaaa atcaccactc
     3841 gatacaggca gcccatcagt ccgggacggc gtcagcggga gagccgttgt aaggcggcag
     3901 actttgctca tgttaccgat gctattcgga agaacggcaa ctaagctgcc gggtttgaaa
     3961 cacggatgat ctcgcggagg gtagcatgtt gattgtaacg atgacagagc gttgctgcct
     4021 gtgatcaccg cggtttcaaa atcggctccg tcgatactat gttatacgcc aactttgaaa
     4081 acaactttga aaaagctgtt ttctggtatt taaggtttta gaatgcaagg aacagtgaat
     4141 tggagttcgt cttgttataa ttagcttctt ggggtatctt taaatactgt agaaaagagg
     4201 aaggaaataa taaatggcta aaatgagaat atcaccggaa ttgaaaaaac tgatcgaaaa
     4261 ataccgctgc gtaaaagata cggaaggaat gtctcctgct aaggtatata agctggtggg
     4321 agaaaatgaa aacctatatt taaaaatgac ggacagccgg tataaaggga ccacctatga
     4381 tgtggaacgg gaaaaggaca tgatgctatg gctggaagga aagctgcctg ttccaaaggt
     4441 cctgcacttt gaacggcatg atggctggag caatctgctc atgagtgagg ccgatggcgt
     4501 cctttgctcg gaagagtatg aagatgaaca aagccctgaa aagattatcg agctgtatgc
     4561 ggagtgcatc aggctctttc actccatcga catatcggat tgtccctata cgaatagctt
     4621 agacagccgc ttagccgaat tggattactt actgaataac gatctggccg atgtggattg
     4681 cgaaaactgg gaagaagaca ctccatttaa agatccgcgc gagctgtatg attttttaaa
     4741 gacggaaaag cccgaagagg aacttgtctt ttcccacggc gacctgggag acagcaacat
     4801 ctttgtgaaa gatggcaaag taagtggctt tattgatctt gggagaagcg gcagggcgga
     4861 caagtggtat gacattgcct tctgcgtccg gtcgatcagg gaggatatcg gggaagaaca
     4921 gtatgtcgag ctattttttg acttactggg gatcaagcct gattgggaga aaataaaata
     4981 ttatatttta ctggatgaat tgttttagta cctagaatgc atgaccaaaa tcccttaacg
     5041 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     5101 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     5161 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     5221 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     5281 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     5341 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     5401 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     5461 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     5521 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     5581 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     5641 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     5701 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     5761 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     5821 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta
     5881 ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat
     5941 ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc
     6001 atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     6061 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     6121 tcaccgtcat caccgaaacg cgcgaggcag ggtgccttga tgtgggcgcc ggcggtcgag
     6181 tggcgacggc gcggcttgtc cgcgccctgg tagattgcct ggccgtaggc cagccatttt
     6241 tgagcggcca gcggccgcga taggccgacg cgaagcggcg gggcgtaggg agcgcagcga
     6301 ccgaagggta ggcgcttttt gcagctcttc ggctgtgcgc tggccagaca gttatgcaca
     6361 ggccaggcgg gttttaagag ttttaataag ttttaaagag ttttaggcgg aaaaatcgcc
     6421 ttttttctct tttatatcag tcacttacat gtgtgaccgg ttcccaatgt acggctttgg
     6481 gttcccaatg tacgggttcc ggttcccaat gtacggcttt gggttcccaa tgtacgtgct
     6541 atccacagga aagagacctt ttcgaccttt ttcccctgct agggcaattt gccctagcat
     6601 ctgctccgta cattaggaac cggcggatgc ttcgccctcg atcaggttgc ggtagcgcat
     6661 gactaggatc gggccagcct gccccgcctc ctccttcaaa tcgtactccg gcaggtcatt
     6721 tgacccgatc agcttgcgca cggtgaaaca gaacttcttg aactctccgg cgctgccact
     6781 gcgttcgtag atcgtcttga acaaccatct ggcttctgcc ttgcctgcgg cgcggcgtgc
     6841 caggcggtag agaaaacggc cgatgccggg atcgatcaaa aagtaatcgg ggtgaaccgt
     6901 cagcacgtcc gggttcttgc cttctgtgat ctcgcggtac atccaatcag ctagctcgat
     6961 ctcgatgtac tccggccgcc cggtttcgct ctttacgatc ttgtagcggc taatcaaggc
     7021 ttcaccctcg gataccgtca ccaggcggcc gttcttggcc ttcttcgtac gctgcatggc
     7081 aacgtgcgtg gtgtttaacc gaatgcaggt ttctaccagg tcgtctttct gctttccgcc
     7141 atcggctcgc cggcagaact tgagtacgtc cgcaacgtgt ggacggaaca cgcggccggg
     7201 cttgtctccc ttcccttccc ggtatcggtt catggattcg gttagatggg aaaccgccat
     7261 cagtaccagg tcgtaatccc acacactggc catgccggcc ggccctgcgg aaacctctac
     7321 gtgcccgtct ggaagctcgt agcggatcac ctcgccagct cgtcggtcac gcttcgacag
     7381 acggaaaacg gccacgtcca tgatgctgcg actatcgcgg gtgcccacgt catagagcat
     7441 cggaacgaaa aaatctggtt gctcgtcgcc cttgggcggc ttcctaatcg acggcgcacc
     7501 ggctgccggc ggttgccggg attctttgcg gattcgatca gcggccgctt gccacgattc
     7561 accggggcgt gcttctgcct cgatgcgttg ccgctgggcg gcctgcgcgg ccttcaactt
     7621 ctccaccagg tcatcaccca gcgccgcgcc gatttgtacc gggccggatg gtttgcgacc
     7681 gctcacgccg attcctcggg cttgggggtt ccagtgccat tgcagggccg gcaggcaacc
     7741 cagccgctta cgcctggcca accgcccgtt cctccacaca tggggcattc cacggcgtcg
     7801 gtgcctggtt gttcttgatt ttccatgccg cctcctttag ccgctaaaat tcatctactc
     7861 atttattcat ttgctcattt actctggtag ctgcgcgatg tattcagata gcagctcggt
     7921 aatggtcttg ccttggcgta ccgcgtacat cttcagcttg gtgtgatcct ccgccggcaa
     7981 ctgaaagttg acccgcttca tggctggcgt gtctgccagg ctggccaacg ttgcagcctt
     8041 gctgctgcgt gcgctcggac ggccggcact tagcgtgttt gtgcttttgc tcattttctc
     8101 tttacctcat taactcaaat gagttttgat ttaatttcag cggccagcgc ctggacctcg
     8161 cgggcagcgt cgccctcggg ttctgattca agaacggttg tgccggcggc ggcagtgcct
     8221 gggtagctca cgcgctgcgt gatacgggac tcaagaatgg gcagctcgta cccggccagc
     8281 gcctcggcaa cctcaccgcc gatgcgcgtg cctttgatcg cccgcgacac gacaaaggcc
     8341 gcttgtagcc ttccatccgt gacctcaatg cgctgcttaa ccagctccac caggtcggcg
     8401 gtggcccata tgtcgtaagg gcttggctgc accggaatca gcacgaagtc ggctgccttg
     8461 atcgcggaca cagccaagtc cgccgcctgg ggcgctccgt cgatcactac gaagtcgcgc
     8521 cggccgatgg ccttcacgtc gcggtcaatc gtcgggcggt cgatgccgac aacggttagc
     8581 ggttgatctt cccgcacggc cgcccaatcg cgggcactgc cctggggatc ggaatcgact
     8641 aacagaacat cggccccggc gagttgcagg gcgcgggcta gatgggttgc gatggtcgtc
     8701 ttgcctgacc cgcctttctg gttaagtaca gcgataacct tcatgcgttc cccttgcgta
     8761 tttgtttatt tactcatcgc atcatatacg cagcgaccgc atgacgcaag ctgttttact
     8821 caaatacaca tcaccttttt agacggcggc gctcggtttc ttcagcggcc aagctggccg
     8881 gccaggccgc cagcttggca tcagacaaac cggccaggat ttcatgcagc cgcacggttg
     8941 agacgtgcgc gggcggctcg aacacgtacc cggccgcgat catctccgcc tcgatctctt
     9001 cggtaatgaa aaacggttcg tcctggccgt cctggtgcgg tttcatgctt gttcctcttg
     9061 gcgttcattc tcggcggccg ccagggcgtc ggcctcggtc aatgcgtcct cacggaaggc
     9121 accgcgccgc ctggcctcgg tgggcgtcac ttcctcgctg cgctcaagtg cgcggtacag
     9181 ggtcgagcga tgcacgccaa gcagtgcagc cgcctctttc acggtgcggc cttcctggtc
     9241 gatcagctcg cgggcgtgcg cgatctgtgc cggggtgagg gtagggcggg ggccaaactt
     9301 cacgcctcgg gccttggcgg cctcgcgccc gctccgggtg cggtcgatga ttagggaacg
     9361 ctcgaactcg gcaatgccgg cgaacacggt caacaccatg cggccggccg gcgtggtggt
     9421 gtcggcccac ggctctgcca ggctacgcag gcccgcgccg gcctcctgga tgcgctcggc
     9481 aatgtccagt aggtcgcggg tgctgcgggc caggcggtct agcctggtca ctgtcacaac
     9541 gtcgccaggg cgtaggtggt caagcatcct ggccagctcc gggcggtcgc gcctggtgcc
     9601 ggtgatcttc tcggaaaaca gcttggtgca gccggccgcg tgcagttcgg cccgttggtt
     9661 ggtcaagtcc tggtcgtcgg tgctgacgcg ggcatagccc agcaggccag cggcggcgct
     9721 cttgttcatg gcgtaatgtc tccggttcta gtcgcaagta ttctacttta tgcgactaaa
     9781 acacgcgaca agaaaacgcc aggaaaaggg cagggcggca gcctgtcgcg taacttagga
     9841 cttgtgcgac atgtcgtttt cagaagacgg ctgcactgaa cgtcagaagc cgactgcact
     9901 atagcagcgg aggggttgga tcaaagtact ttgatcccga ggggaaccct gtggttggca
     9961 tgcacataca aatggacgaa cggataaacc ttttcacgcc cttttaaata tccgattatt
    10021 ctaataaacg ctcttttctc ttaggtttac ccgccaatat atcctgtcaa acactgatag
    10081 tttaaactga aggcgggaaa cgacaatctg atccaagctc aagctgctct agcattcgcc
    10141 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca
    10201 gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca
    10261 gtcacgacgt tgtaaaacga cggccagtgc caagcttgca tgcctgcagg tcctgctgag
    10321 cctcgacatg ttgtcgcaaa attcgccctg gacccgccca acgatttgtc gtcactgtca
    10381 aggtttgacc tgcacttcat ttggggccca catacaccaa aaaaatgctg cataattctc
    10441 ggggcagcaa gtcggttacc cggccgccgt gctggaccgg gttgaatggt gcccgtaact
    10501 ttcggtagag cggacggcca atactcaact tcaaggaatc tcacccatgc gcgccggcgg
    10561 ggaaccggag ttcccttcag tgagcgttat tagttcgccg ctcggtgtgt cgtagatact
    10621 agcccctggg gcacttttga aatttgaata agatttatgt aatcagtctt ttaggtttga
    10681 ccggttctgc cgcttttttt aaaattggat ttgtaataat aaaacgcaat tgtttgttat
    10741 tgtggcgctc tatcatagat gtcgctataa acctattcag cacaatatat tgttttcatt
    10801 ttaatattgt acatataagt agtagggtac aatcagtaaa ttgaacggag aatattattc
    10861 ataaaaatac gatagtaacg ggtgatatat tcattagaat gaaccgaaac cggcggtaag
    10921 gatctgagct acacatgctc aggtttttta caacgtgcac aacagaattg aaagcaaata
    10981 tcatgcgatc ataggcgtct cgcatatctc attaaagcag gactctaggg actagtcccg
    11041 ggtcttaatt aagtttgcaa gcagcagatt acgggatacc tgtccgcctt tcttaattaa
    11101 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aaggagatcc tttggatcac
    11161 aaaaaaaagc ccgctcatta ggcgggttaa attcccgggt actgcagccg attcattaat
    11221 gcaggttaac ctggcttatc gaaattaata cgactcacta tagggagacc ggcagatctg
    11281 atccctactc caaaaatgtc aaagatacag tctcagaaga ccaaagggct at
//
