LOCUS       sequence-167313-        1052 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For Flpe/FRT-based Supernova-GFP, pK068 should be used with pK036. 
            The GFP fragment of pK068 can be replaced with another gene by SalI 
            and EcoRV. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Luo W, Mizuno H, Iwata R, Nakazawa S, Yasuda K, Itohara S, Iwasato T
  TITLE     Supernova: A Versatile Vector System for Single-Cell Labeling and 
            Gene Function Studies in vivo.
  JOURNAL   Sci Rep. 2016 Oct 24;6:35747. doi: 10.1038/srep35747.
  PUBMED    27775045
REFERENCE   2  (bases 1 to 1052)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-10-24"; volume: "6:35747. doi"; pages: " 
            10.1038/srep35747"
COMMENT     Sequence Label: pK068.CAG-FRT-stop-FRT-EGFP-ires-tTA-WPRE
            (Sup...
FEATURES             Location/Qualifiers
     source          1..1052
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(74..82,83..118,119..121,122..157,158..160,
                     161..196,197..202,203..820))
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=tTA-Advanced
                     /note="In the Tet-Off(R) system, tTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(973..992)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
ORIGIN
        1 cagtcaatct ttcacaaatt ttgtaatcca gaggttgagc ggccgaaggg cgaattcgcg
       61 gccgcttact tagttacccg gggagcatgt caaggtcaaa atcgtcaaga gcgtcagcag
      121 gcagcatatc aaggtcaaag tcgtcaaggg catcggctgg gagcatgtct aagtcaaaat
      181 cgtcaagggc gtcggccggc ccgccgcttt cgcactttag ctgtttctcc aggccacata
      241 tgattagttc caggccgaaa aggaaggcag gttcggctcc ctgatggtcg aacagctcaa
      301 ttgcttgtct cagaagtggg ggcatagaat cggtggtagg tgtctctctt tcctcttttg
      361 ctacttgatg ctcctgatcc tccaatacgc agcccagtgt aaagtggccc acggcggaca
      421 gagcgtacag tgcgttctcc agggagaagc cttgctgaca caggaacgcg agctgatttt
      481 ccagggtttc gtactgtttc tctgttgggc gggtgccgag atgcacttta gccccgtcgc
      541 gatgtgagag gagagcacag cggaatgact tggcgttgtt ccgcagaaag tcttgccatg
      601 actcgccttc cagggggcag aagtgggtat gatgcctgtc cagcatctcg attgccaggg
      661 catcgagcag ggcccgcttg ttcttcacgt gccagtacag ggtaggctgc tcaactccca
      721 gcttttgagc gagtttcctt gtcgtcaggc cttcgatacc gacttcattg agtaattcca
      781 gagcagagtt tatgactttg ctcttgtcca gtctagacat cgtgtttttc aaaggaaacc
      841 acgtccccgt ggttcggggg gcctagacgt ttttttaacc tcgactaaac acatgtaaag
      901 catgtgcacc gaggccccag atcagatccc atacaatggg gtaccttctg ggcatccttc
      961 agccccttgt tgaatacgct tgaggagagc cattttactc tttccacaac tatccaactc
     1021 acaacgtggc actggggttg tgccgccttt gc
//
