LOCUS       sequence-167314-       10264 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For Flpe/FRT-based Supernova-GFP, pK068 should be used with pK036. 
            The GFP fragment of pK068 can be replaced with another gene by SalI 
            and EcoRV. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10264)
  AUTHORS   Luo W, Mizuno H, Iwata R, Nakazawa S, Yasuda K, Itohara S, Iwasato T
  TITLE     Supernova: A Versatile Vector System for Single-Cell Labeling and 
            Gene Function Studies in vivo.
  JOURNAL   Sci Rep. 2016 Oct 24;6:35747. doi: 10.1038/srep35747.
  PUBMED    27775045
REFERENCE   2  (bases 1 to 10264)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-10-24"; volume: "6:35747. doi"; pages: " 
            10.1038/srep35747"
COMMENT     Sequence Label: pK068.CAG-FRT-stop-FRT-EGFP-ires-tTA-WPRE
            (Sup...
FEATURES             Location/Qualifiers
     source          1..10264
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        10..389
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        392..667
                     /label=chicken beta-actin promoter
     intron          668..1676
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1600..1622
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     protein_bind    complement(1733..1766)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     intron          2300..2365
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2495..2515
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2940..3074
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2977..2996)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3031..3050
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(3116..3149)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     regulatory      3158..3167
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3164..3166,3167..3169,3170..3883)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3164..3166,3167..3169,3170..3880)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3209..3230)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3470..3489)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3817..3838
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4083..4100)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4310..4329
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(4482..5099,5100..5105,5106..5141,5142..5144,5145..5180,
                     5181..5183,5184..5219,5220..5228)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=tTA-Advanced
                     /note="In the Tet-Off(R) system, tTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(5315..5335)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5733..5744)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5873..5892)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5938..5993
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5992..6011)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     rep_origin      complement(6376..6964)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6456..6475)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    7293..7340
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     7365..7384
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(7572..8360)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDQEHQGL
                     APAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALA
                     TRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7683..7702)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     8287..8306
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(8395..8755)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8409..8547
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        8564..8755
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     8712..8732
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             complement(join(8815..9606,9607..9675))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9438..9457
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9676..9780)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      9807..10262
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9894..9913)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10104..10125
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gcgcgcgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
       61 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
      121 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
      181 caatagggac tttccattga cgtcaatggg tggactattt acggtaaact gcccacttgg
      241 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
      301 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
      361 tctacgtatt agtcatcgct attaccatgg gtcgaggtga gccccacgtt ctgcttcact
      421 ctccccatct cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt
      481 tgtgcagcga tgggggcggg gggggggggg gcgcgcgcca ggcggggcgg ggcggggcga
      541 ggggcggggc ggggcgaggc ggagaggtgc ggcggcagcc aatcagagcg gcgcgctccg
      601 aaagtttcct tttatggcga ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg
      661 gcgggcggga gtcgctgcgt tgccttcgcc ccgtgccccg ctccgcgccg cctcgcgccg
      721 cccgccccgg ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc
      781 tcctccgggc tgtaattagc gcttggttta atgacggctc gtttcttttc tgtggctgcg
      841 tgaaagcctt aaagggctcc gggagggccc tttgtgcggg ggggagcggc tcggggggtg
      901 cgtgcgtgtg tgtgtgcgtg gggagcgccg cgtgcggccc gcgctgcccg gcggctgtga
      961 gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcgtg tgcgcgaggg gagcgcggcc
     1021 gggggcggtg ccccgcggtg cgggggggct gcgaggggaa caaaggctgc gtgcggggtg
     1081 tgtgcgtggg ggggtgagca gggggtgtgg gcgcggcggt cgggctgtaa cccccccctg
     1141 cacccccctc cccgagttgc tgagcacggc ccggcttcgg gtgcggggct ccgtgcgggg
     1201 cgtggcgcgg ggctcgccgt gccgggcggg gggtggcggc aggtgggggt gccgggcggg
     1261 gcggggccgc ctcgggccgg ggagggctcg ggggaggggc gcggcggccc cggagcgccg
     1321 gcggctgtcg aggcgcggcg agccgcagcc attgcctttt atggtaatcg tgcgagaggg
     1381 cgcagggact tcctttgtcc caaatctggc ggagccgaaa tctgggaggc gccgccgcac
     1441 cccctctagc gggcgcgggc gaagcggtgc ggcgccggca ggaaggaaat gggcggggag
     1501 ggccttcgtg cgtcgccgcg ccgccgtccc cttctccatc tccagcctcg gggctgccgc
     1561 agggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga
     1621 ccggcggctc tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc
     1681 tgggcaacgt gctggttgtt gtgctgtctc atcattttgg caaagaattc tagaagttcc
     1741 tatactttct agagaatagg aacttcatta agggttccgg atcctcgggg acaccaaata
     1801 tggcgatctc ggccttttcg tttcttggag ctgggacatg tttgccatcg atccatctac
     1861 caccagaacg gccgttagat ctgctgccac cgttgtttcc accgaagaaa ccaccgttgc
     1921 cgtaaccacc acgacggttg ttgctaaaga agctgccacc gccacggcca ccgttgtagc
     1981 cgccgttgtt gttattgtag ttgctcatgt tatttctggc acttcttggt tttcctctta
     2041 agtgaggagg aacataacca ttctcgttgt tgtcgttgat gcttaaattt tgcacttgtt
     2101 cgctcagttc agccataata tgaaatgctt ttcttgttgt tcttacggaa taccacttgc
     2161 cacctatcac cacaactaac tttttcccgt tcctccatct cttttatatt ttttttctcg
     2221 agggatcttt gtgaaggaac cttacttctg tggtgtgaca taattggaca aactacctac
     2281 agagatttaa agctctaagg taaatataaa atttttaagt gtataatgtg ttaaactact
     2341 gattctaatt gtttgtgtat tttagattcc aacctatgga actgatgaat gggagcagtg
     2401 gtggaatgcc tttaatgagg aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga
     2461 tgaggctact gctgactctc aacattctac tcctccaaaa aagaagagaa aggtagaaga
     2521 ccccaaggac tttccttcag aattgctaag ttttttgagt catgctgtgt ttagtaatag
     2581 aactcttgct tgctttgcta tttacaccac aaaggaaaaa gctgcactgc tatacaagaa
     2641 aattatggaa aaatattctg taacctttat aagtaggcat aacagttata atcataacat
     2701 actgtttttt cttactccac acaggcatag agtgtctgct attaataact atgctcaaaa
     2761 attgtgtacc tttagctttt taatttgtaa aggggttaat aaggaatatt tgatgtatag
     2821 tgccttgact agagatcata atcagccata ccacatttgt agaggtttta cttgctttaa
     2881 aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta
     2941 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     3001 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     3061 atcatgtctg gatctgacat ggtaagtaag cttgggctgc aggtcgaggg acctagaagt
     3121 tcctatactt tctagagaat aggaacttca tgtcgacgcc accatggtga gcaagggcga
     3181 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     3241 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     3301 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
     3361 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
     3421 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     3481 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     3541 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     3601 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
     3661 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     3721 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
     3781 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     3841 cgccgccggg atcactctcg gcatggacga gctgtacaag taagatatcc tagatcgagt
     3901 taattaagga ttccgcccct ctccctcccc cccccctaac gttactggcc gaagccgctt
     3961 ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg
     4021 caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc
     4081 ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga
     4141 agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc
     4201 tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc
     4261 acaaccccag tgccacgttg tgagttggat agttgtggaa agagtaaaat ggctctcctc
     4321 aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga
     4381 tctggggcct cggtgcacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg
     4441 ccccccgaac cacggggacg tggtttcctt tgaaaaacac gatgtctaga ctggacaaga
     4501 gcaaagtcat aaactctgct ctggaattac tcaatgaagt cggtatcgaa ggcctgacga
     4561 caaggaaact cgctcaaaag ctgggagttg agcagcctac cctgtactgg cacgtgaaga
     4621 acaagcgggc cctgctcgat gccctggcaa tcgagatgct ggacaggcat catacccact
     4681 tctgccccct ggaaggcgag tcatggcaag actttctgcg gaacaacgcc aagtcattcc
     4741 gctgtgctct cctctcacat cgcgacgggg ctaaagtgca tctcggcacc cgcccaacag
     4801 agaaacagta cgaaaccctg gaaaatcagc tcgcgttcct gtgtcagcaa ggcttctccc
     4861 tggagaacgc actgtacgct ctgtccgccg tgggccactt tacactgggc tgcgtattgg
     4921 aggatcagga gcatcaagta gcaaaagagg aaagagagac acctaccacc gattctatgc
     4981 ccccacttct gagacaagca attgagctgt tcgaccatca gggagccgaa cctgccttcc
     5041 ttttcggcct ggaactaatc atatgtggcc tggagaaaca gctaaagtgc gaaagcggcg
     5101 ggccggccga cgcccttgac gattttgact tagacatgct cccagccgat gcccttgacg
     5161 actttgacct tgatatgctg cctgctgacg ctcttgacga ttttgacctt gacatgctcc
     5221 ccgggtaact aagtaagcgg ccgcgaattc gcccttcggc cgctcaacct ctggattaca
     5281 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     5341 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     5401 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     5461 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     5521 cctgtcagct cctttccgga actttcgctt tccccctccc tattgccacg gcggaactca
     5581 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     5641 tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctatgtt gccacctgga
     5701 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     5761 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     5821 gtcggatctc cctttgggcc gcctccccgc agcggccgcg gcgactcgag gatctttttc
     5881 cctctgccaa aaattatggg gacatcatga agccccttga gcatctgact tctggctaat
     5941 aaaggaaatt tattttcatt gcaatagtgt gttggaattt tttgtgtctc tcactcggaa
     6001 ggacatatgg gagggcaaat catttaaaac atcagaatga gtatttggtt tagagtttgg
     6061 caacatatgc catatgctgg ctgccatgaa caaaggtggc tataaagagg tcatcagtat
     6121 atgaaacagc cccctgctgt ccattcctta ttccatagaa aagccttgac ttgaggttag
     6181 atttttttta tattttgttt tgtgttattt ttttctttaa catccctaaa attttcctta
     6241 catgttttac tagccagatt tttcctcctc tcctgactac tcccagtcat agctgtccct
     6301 cttctcttat gaagccatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     6361 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     6421 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     6481 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     6541 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     6601 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     6661 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     6721 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     6781 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
     6841 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     6901 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     6961 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     7021 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     7081 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa cctgaggcta tggcagggcc
     7141 tgccgccccg acgttggctg cgagccctgg gccttcaccc gaacttgggg ggtggggtgg
     7201 ggaaaaggaa gaaacgcggg cgtattggcc ccaatggggt ctcggtgggg tatcgacaga
     7261 gtgccagccc tgggaccgaa ccccgcgttt atgaacaaac gacccaacac cgtgcgtttt
     7321 attctgtctt tttattgccg tcatagcgcg ggttccttcc ggtattgtct ccttccgtgt
     7381 ttcagttagc ctccccctag ggtgggcgaa gaactccagc atgagatccc cgcgctggag
     7441 gatcatccag ccggcgtccc ggaaaacgat tccgaagccc aacctttcat agaaggcggc
     7501 ggtggaatcg aaatctcgtg atggcaggtt gggcgtcgct tggtcggtca tttcgaaccc
     7561 cagagtcccg ctcagaagaa ctcgtcaaga aggcgataga aggcgatgcg ctgcgaatcg
     7621 ggagcggcga taccgtaaag cacgaggaag cggtcagccc attcgccgcc aagctcttca
     7681 gcaatatcac gggtagccaa cgctatgtcc tgatagcggt ccgccacacc cagccggcca
     7741 cagtcgatga atccagaaaa gcggccattt tccaccatga tattcggcaa gcaggcatcg
     7801 ccatgggtca cgacgagatc ctcgccgtcg ggcatgctcg ccttgagcct ggcgaacagt
     7861 tcggctggcg cgagcccctg atgctcttga tcatcctgat cgacaagacc ggcttccatc
     7921 cgagtacgtg ctcgctcgat gcgatgtttc gcttggtggt cgaatgggca ggtagccgga
     7981 tcaagcgtat gcagccgccg cattgcatca gccatgatgg atactttctc ggcaggagca
     8041 aggtgagatg acaggagatc ctgccccggc acttcgccca atagcagcca gtcccttccc
     8101 gcttcagtga caacgtcgag cacagctgcg caaggaacgc ccgtcgtggc cagccacgat
     8161 agccgcgctg cctcgtcttg cagttcattc agggcaccgg acaggtcggt cttgacaaaa
     8221 agaaccgggc gcccctgcgc tgacagccgg aacacggcgg catcagagca gccgattgtc
     8281 tgttgtgccc agtcatagcc gaatagcctc tccacccaag cggccggaga acctgcgtgc
     8341 aatccatctt gttcaatcat gcgaaacgat cctcatcctg tctcttgatc gatctttgca
     8401 aaagcctagg cctccaaaaa agcctcctca ctacttctgg aatagctcag aggccgagga
     8461 ggcggcctcg gcctctgcat aaataaaaaa aattagtcag ccatggggcg gagaatgggc
     8521 ggaactgggc ggagttaggg gcgggatggg cggagttagg ggcgggacta tggttgctga
     8581 ctaattgaga tgcatgcttt gcatacttct gcctgctggg gagcctgggg actttccaca
     8641 cctggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg gggagcctgg
     8701 ggactttcca caccctaact gacacacatt ccacagctgg ttctttccgc ctcaggactc
     8761 ttcctttttc aataaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     8821 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     8881 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     8941 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     9001 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     9061 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     9121 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     9181 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     9241 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     9301 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     9361 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     9421 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     9481 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     9541 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     9601 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     9661 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     9721 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     9781 cacatttccc cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg
     9841 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     9901 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     9961 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
    10021 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
    10081 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
    10141 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
    10201 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat
    10261 ttac
//
