LOCUS       sequence-167317-        1027 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For nucleus-specific RFP labeling in single cells, pK098 should be 
            used with pK031..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Luo W, Mizuno H, Iwata R, Nakazawa S, Yasuda K, Itohara S, Iwasato T
  TITLE     Supernova: A Versatile Vector System for Single-Cell Labeling and 
            Gene Function Studies in vivo.
  JOURNAL   Sci Rep. 2016 Oct 24;6:35747. doi: 10.1038/srep35747.
  PUBMED    27775045
REFERENCE   2  (bases 1 to 1027)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-10-24"; volume: "6:35747. doi"; pages: " 
            10.1038/srep35747"
COMMENT     Sequence Label: pK098.CAG-loxP-stop-loxP-nlstagRFP-ires-tTA-WP...
FEATURES             Location/Qualifiers
     source          1..1027
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(11..44)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     intron          578..643
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             773..793
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 aagaattcta ataacttcgt atagcataca ttatacgaag ttatattaag ggttccggat
       61 cctcggggac accaaatatg gcgatctcgg ccttttcgtt tcttggagct gggacatgtt
      121 tgccatcgat ccatctacca ccagaacggc cgttagatct gctgccaccg ttgtttccac
      181 cgaagaaacc accgttgccg taaccaccac gacggttgtt gctaaagaag ctgccaccgc
      241 cacggccacc gttgtagccg ccgttgttgt tattgtagtt gctactgtta tttctggcac
      301 ttcttggttt tcctcttaag tgaggaggaa cataaccatt ctcgttgttg tcgttgatgc
      361 ttaaattttg cacttgttcg ctcagttcag ccataatatg aaatgctttt cttgttgttc
      421 ttacggaata ccacttgcca cctatcacca caactaactt tttcccgttc ctccatctct
      481 tttatatttt ttttctcgag ggatctttgt gaaggaacct tacttctgtg gtgtgacata
      541 attggacaaa ctacctacag agatttaaag ctctaaggta aatataaaat ttttaagtgt
      601 ataatgtgtt aaactactga ttctaattgt ttgtgtattt tagattccaa cctatggaac
      661 tgatgaatgg gagcagtggt ggaatgcctt taatgaggaa aacctgtttt gctcagaaga
      721 aatgccatct agtgatgatg aggctactgc tgactctcaa cattctactc ctccaaaaaa
      781 gaagagaaag gtagaagacc ccaaggactt tccttcagaa ttgctaagtt ttttgagtca
      841 tgctgtgttt agtaatagaa ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc
      901 tgcactgcta tacaagaaaa ttatggaaaa atattctgta acctttataa gtaggcataa
      961 cagttataat cataacatac tgttttttct tactccacac aggcatagag tgtctgctat
     1021 taataac
//
