LOCUS       sequence-167322-         857 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For nucleus-specific RFP labeling in single cells, pK098 should be 
            used with pK031..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Luo W, Mizuno H, Iwata R, Nakazawa S, Yasuda K, Itohara S, Iwasato T
  TITLE     Supernova: A Versatile Vector System for Single-Cell Labeling and 
            Gene Function Studies in vivo.
  JOURNAL   Sci Rep. 2016 Oct 24;6:35747. doi: 10.1038/srep35747.
  PUBMED    27775045
REFERENCE   2  (bases 1 to 857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-10-24"; volume: "6:35747. doi"; pages: " 
            10.1038/srep35747"
COMMENT     Sequence Label: pK098.CAG-loxP-stop-loxP-nlstagRFP-ires-tTA-WP...
FEATURES             Location/Qualifiers
     source          1..857
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(52..85)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             101..121
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             125..817
                     /codon_start=1
                     /product="red fluorescent protein from Entacmaea 
                     quadricolor"
                     /label=TurboRFP
                     /note="mammalian codon-optimized"
                     /translation="SELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKVV
                     EGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTAT
                     QDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALKL
                     VGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYCD
                     LPSKLGHR"
ORIGIN
        1 tgtctggatc tgacatggta agtaagcttg ggctgcaggt cgagggacct aataacttcg
       61 tatagcatac attatacgaa gttatatgtc gaccatgggc ccaaagaaga agagaaaggt
      121 ttcgagcgag ctgatcaagg agaacatgca catgaagctg tacatggagg gcaccgtgaa
      181 caaccaccac ttcaagtgca catccgaggg cgaaggcaag ccctacgagg gcacccagac
      241 catgaagatc aaggtggtcg agggcggccc tctccccttc gccttcgaca tcctggctac
      301 cagcttcatg tacggcagca aagccttcat caaccacacc cagggcatcc ccgacttctt
      361 taagcagtcc ttccctgagg gcttcacatg ggagagaatc accacatacg aagacggggg
      421 cgtgctgacc gctacccagg acaccagctt ccagaacggc tgcatcatct acaacgtcaa
      481 gatcaacggg gtgaacttcc catccaacgg ccctgtgatg cagaagaaaa cacgcggctg
      541 ggaggccaac accgagatgc tgtaccccgc tgacggcggc ctgagaggcc acagccagat
      601 ggccctgaag ctcgtgggcg ggggctacct gcactgctcc ttcaagacca catacagatc
      661 caagaaaccc gctaagaacc tcaagatgcc cggcttccac ttcgtggacc acagactgga
      721 aagaatcaag gaggccgaca aagagaccta cgtcgagcag cacgagatgg ctgtggccaa
      781 gtactgcgac ctccctagca aactggggca cagatgatgc agatatccta gatcgagtta
      841 attaaggatt ccgcccc
//
