LOCUS       sequence-167349-        4974 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing CTCF , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4974)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4974)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_CTCF_p.N568K
FEATURES             Location/Qualifiers
     source          1..4974
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2374..2473)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2490..2509)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2491..2509)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2514..2530)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2514..2530)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2961..3752
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3845..4433
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4334..4353
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4587..4604
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4763..4790)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4882..4968)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcat ggaaggtgat gcagtcgaag ccattgtgga ggagtccgaa acttttatta
      241 aaggaaagga gagaaagact taccagagac gccgggaagg gggccaggaa gaagatgcct
      301 gccacttacc ccagaaccag acggatgggg gtgaggtggt ccaggatgtc aacagcagtg
      361 tacagatggt gatgatggaa cagctggacc ccacccttct tcagatgaag actgaagtaa
      421 tggagggcac agtggctcca gaagcagagg ctgctgtgga cgatacccag attataactt
      481 tacaggttgt aaatatggag gaacagccca taaacatagg agaacttcag cttgttcaag
      541 tacctgttcc tgtgactgta cctgttgcta ccacttcagt agaagaactt cagggggctt
      601 atgaaaatga agtgtctaaa gagggccttg cggaaagtga acccatgata tgccacaccc
      661 tacctttgcc tgaagggttt caggtggtta aagtgggggc caatggagag gtggagacac
      721 tagaacaagg ggaacttcca ccccaggaag atcctagttg gcaaaaagac ccagactatc
      781 agccaccagc caaaaaaaca aagaaaacca aaaagagcaa actgcgttat acagaggagg
      841 gcaaagatgt agatgtgtct gtctacgatt ttgaggaaga acagcaggag ggtctgctat
      901 cagaggttaa tgcagagaaa gtggttggta atatgaagcc tccaaagcca acaaaaatta
      961 aaaagaaagg tgtaaagaag acattccagt gtgagctttg cagttacacg tgtccacggc
     1021 gttcaaattt ggatcgtcac atgaaaagcc acactgatga gagaccacac aagtgccatc
     1081 tctgtggcag ggcattcaga acagtcaccc tcctgaggaa tcaccttaac acacacacag
     1141 gtactcgtcc tcacaagtgc ccagactgcg acatggcctt tgtgaccagt ggagaattgg
     1201 ttcggcatcg tcgttacaaa cacacccacg agaagccatt caagtgttcc atgtgcgatt
     1261 acgccagtgt agaagtcagc aaattaaaac gtcacattcg ctctcatact ggagagcgtc
     1321 cgtttcagtg cagtttgtgc agttatgcca gcagggacac atacaagctg aaaaggcaca
     1381 tgagaaccca ttcaggggaa aagccttatg aatgttatat ttgtcatgct cggtttaccc
     1441 aaagtggtac catgaagatg cacattttac agaagcacac agaaaatgtg gccaaatttc
     1501 actgtcccca ctgtgacaca gtcatagccc gaaaaagtga tttgggtgtc cacttgcgaa
     1561 agcagcattc ctatattgag caaggcaaga aatgccgtta ctgtgatgct gtgtttcatg
     1621 agcgctatgc cctcatccag catcagaagt cacacaagaa tgagaagcgc tttaagtgtg
     1681 accagtgtga ttacgcttgt agacaggaga ggcacatgat catgcacaag cgcacccaca
     1741 ccggggagaa gccttacgcc tgcagccact gcgataagac cttccgccag aagcagcttc
     1801 tcgacatgca cttcaagcgc tatcacgacc ccaacttcgt ccctgcggct tttgtctgtt
     1861 ctaagtgtgg gaaaacattt acacgtcgga aaaccatggc aagacatgct gataattgtg
     1921 ctggcccaga tggcgtagag ggggaaaatg gaggagaaac gaagaagagt aaacgtggaa
     1981 gaaaaagaaa gatgcgctct aagaaagaag attcctctga cagtgaaaat gctgaaccag
     2041 atctggacga caatgaggat gaggaggagc ctgccgtaga aattgaacct gagccagagc
     2101 ctcagcctgt gaccccagcc ccaccacccg ccaagaagcg gagaggacga ccccctggca
     2161 gaaccaacca gcccaaacag aaccagccaa cagctatcat tcaggttgaa gaccagaata
     2221 caggtgcaat tgagaacatt atagttgaag taaaaaaaga gccagatgct gagcccgcag
     2281 agggagagga agaggaggcc cagccagctg ccacagatgc ccccaacgga gacctcacgc
     2341 ccgagatgat cctcagcatg atggaccggt gattgccaac tttcttgtac aaagttggca
     2401 ttataagaaa gcattgctta tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata
     2461 aaatcattat ttgccatcca gctgatatcc cctatagtga gtcgtattac atggtcatag
     2521 ctgtttcctg gcagctctgg cccgtgtctc aaaatctctg atgttacatt gcacaagata
     2581 aaaatatatc atcatgcctc ctctagacca gccaggacag aaatgcctcg acttcgctgc
     2641 tacccaaggt tgccgggtga cgcacaccgt ggaaacggat gaaggcacga acccagtgga
     2701 cataagcctg ttcggttcgt aagctgtaat gcaagtagcg tatgcgctca cgcaactggt
     2761 ccagaacctt gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt
     2821 atgactgttt ttttggggta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg
     2881 ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc
     2941 ctaaaacaaa gttaaacatt atgagggaag cggtgatcgc cgaagtatcg actcaactat
     3001 cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt
     3061 acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg
     3121 tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt
     3181 cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg
     3241 acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc
     3301 gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct
     3361 tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct
     3421 ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga
     3481 actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt
     3541 ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg
     3601 agcgcctgcc ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac
     3661 aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc cactacgtga
     3721 aaggcgagat caccaaggta gtcggcaaat aaccctcgag ccacccatga ccaaaatccc
     3781 ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     3841 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     3901 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     3961 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     4021 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     4081 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     4141 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     4201 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     4261 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     4321 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     4381 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     4441 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     4501 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     4561 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     4621 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     4681 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatacgcgt accgctagcc
     4741 aggaagagtt tgtagaaacg caaaaaggcc atccgtcagg atggccttct gcttagtttg
     4801 atgcctggca gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac
     4861 gttcaaatcc gctcccggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca
     4921 gataaaacga aaggcccagt cttccgactg agcctttcgt tttatttgat gcct
//
