LOCUS       sequence-167353-        4158 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing TMPO , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4158)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4158)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_TMPO_p.D185H
FEATURES             Location/Qualifiers
     source          1..4158
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1558..1657)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1674..1693)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1675..1693)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1698..1714)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1698..1714)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2145..2936
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3029..3617
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3518..3537
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3771..3788
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3947..3974)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4066..4152)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcac catgccggag ttcctggaag acccctcggt cctgacaaaa gacaagttga
      241 agagtgagtt ggtcgccaac aatgtgacgc tgccggccgg ggagcagcgc aaagacgtgt
      301 acgtccagct ctacctgcag cacctcacgg ctcgcaaccg gccgccgctc cccgccggca
      361 ccaacagcaa ggggcccccg gacttctcca gtgacgaaga gcgcgagccc accccggtcc
      421 tcggctctgg ggccgccgcc gcgggccgga gccgagcagc cgtcggcagg aaagccacaa
      481 aaaaaactga taaacccaga caagaagata aagatgatct agatgtaaca gagctcacta
      541 atgaagatct tttggatcag cttgtgaaat acggagtgaa tcctggtcct attgtgggaa
      601 caaccaggaa gctatatgag aaaaagcttt tgaaactgag ggaacaagga acagaatcaa
      661 gatcttctac tcctctgcca acaatttctt cttcagcaga aaatacaagg cagaatggaa
      721 gtaatgattc tgacagatac agtcacaatg aagaagactc taaaatagag ctcaagcttg
      781 agaagagaga accactaaag ggcagagcaa agactccagt aacactcaag caaagaagag
      841 ttgagcacaa tcagagctat tctcaagctg gaataactga gactgaatgg acaagtggat
      901 cttcaaaagg cggacctctg caggcattaa ctagggaatc tacaagaggg tcaagaagaa
      961 ctccaaggaa aagggtggaa acttcagaac attttcgtat agatggtcca gtaatttcag
     1021 agagtactcc catagctgaa actataatgg cttcaagcaa cgaatcctta gttgtcaata
     1081 gggtgactgg aaatttcaag catgcatctc ctattctgcc aatcactgaa ttctcagaca
     1141 tacccagaag agcaccaaag aaaccattga caagagctga agtgggagaa aaaacagagg
     1201 aaagaagagt agaaagggat attcttaagg aaatgttccc ctatgaagca tctacaccaa
     1261 caggaattag tgctagttgc cgcagaccaa tcaaaggggc tgcaggccgg ccattagaac
     1321 tcagtgattt caggatggag gagtcttttt catctaaata tgttcctaag tatgttccct
     1381 tggcagatgt caagtcagaa aagacaaaaa agggacgctc cattcccgta tggataaaaa
     1441 ttttgctgtt tgttgttgtg gcagtttttt tgtttttggt ctatcaagct atggaaacca
     1501 accaagtaaa tcccttctct aattttcttc atgttgaccc tagaaaatcc aactgattgc
     1561 caactttctt gtacaaagtt ggcattataa gaaagcattg cttatcaatt tgttgcaacg
     1621 aacaggtcac tatcagtcaa aataaaatca ttatttgcca tccagctgat atcccctata
     1681 gtgagtcgta ttacatggtc atagctgttt cctggcagct ctggcccgtg tctcaaaatc
     1741 tctgatgtta cattgcacaa gataaaaata tatcatcatg cctcctctag accagccagg
     1801 acagaaatgc ctcgacttcg ctgctaccca aggttgccgg gtgacgcaca ccgtggaaac
     1861 ggatgaaggc acgaacccag tggacataag cctgttcggt tcgtaagctg taatgcaagt
     1921 agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg
     1981 cagtggcggt tttcatggct tgttatgact gtttttttgg ggtacagtct atgcctcggg
     2041 catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg
     2101 atgttacgca gcagggcagt cgccctaaaa caaagttaaa cattatgagg gaagcggtga
     2161 tcgccgaagt atcgactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac
     2221 cgacgttgct ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca
     2281 gtgatattga tttgctggtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt
     2341 tgatcaacga ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg
     2401 tagaagtcac cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg
     2461 aactgcaatt tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca
     2521 cgatcgacat tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg
     2581 taggtccagc ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc
     2641 taaatgaaac cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg
     2701 tagtgcttac gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg
     2761 atgtcgctgc cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg
     2821 aagctagaca ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt
     2881 tggaagaatt tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc aaataaccct
     2941 cgagccaccc atgaccaaaa tcccttaacg tgagttacgc gtcgttccac tgagcgtcag
     3001 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     3061 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     3121 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     3181 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     3241 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     3301 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     3361 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     3421 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     3481 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     3541 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     3601 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     3661 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     3721 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     3781 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     3841 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     3901 caattaatac gcgtaccgct agccaggaag agtttgtaga aacgcaaaaa ggccatccgt
     3961 caggatggcc ttctgcttag tttgatgcct ggcagtttat ggcgggcgtc ctgcccgcca
     4021 ccctccgggc cgttgcttca caacgttcaa atccgctccc ggcggatttg tcctactcag
     4081 gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtcttccg actgagcctt
     4141 tcgttttatt tgatgcct
//
