LOCUS       sequence-167354-        4128 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing TUBB , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4128)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4128)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_TUBB_p.R359P
FEATURES             Location/Qualifiers
     source          1..4128
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1528..1627)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1644..1663)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1645..1663)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1668..1684)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1668..1684)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2115..2906
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2999..3587
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3488..3507
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3741..3758
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3917..3944)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4036..4122)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcac catgagggaa atcgtgcaca tccaggctgg tcagtgtggc aaccagatcg
      241 gtgccaagtt ctgggaggtg atcagtgatg aacatggcat cgaccccacc ggcacctacc
      301 acggggacag cgacctgcag ctggaccgca tctctgtgta ctacaatgaa gccacaggtg
      361 gcaaatatgt tcctcgtgcc atcctggtgg atctagaacc tgggaccatg gactctgttc
      421 gctcaggtcc ttttggccag atctttagac cagacaactt tgtatttggt cagtctgggg
      481 caggtaacaa ctgggccaaa ggccactaca cagagggcgc cgagctggtt gattctgtcc
      541 tggatgtggt acggaaggag gcagagagct gtgactgcct gcagggcttc cagctgaccc
      601 actcactggg cgggggcaca ggctctggaa tgggcactct ccttatcagc aagatccgag
      661 aagaataccc tgatcgcatc atgaatacct tcagtgtggt gccttcaccc aaagtgtctg
      721 acaccgtggt cgagccctac aatgccaccc tctccgtcca tcagttggta gagaatactg
      781 atgagaccta ttgcattgac aacgaggccc tctatgatat ctgcttccgc actctgaagc
      841 tgaccacacc aacctacggg gatctgaacc accttgtctc agccaccatg agtggtgtca
      901 ccacctgcct ccgtttccct ggccagctca atgctgacct ccgcaagttg gcagtcaaca
      961 tggtcccctt cccacgtctc catttcttta tgcctggctt tgcccctctc accagccgtg
     1021 gaagccagca gtatcgagct ctcacagtgc cggaactcac ccagcaggtc ttcgatgcca
     1081 agaacatgat ggctgcctgt gacccccgcc acggccgata cctcaccgtg gctgctgtct
     1141 tccgtggtcg gatgtccatg aaggaggtcg atgagcagat gcttaacgtg cagaacaaga
     1201 acagcagcta ctttgtggaa tggatcccca acaatgtcaa gacagccgtc tgtgacatcc
     1261 cacctcctgg cctcaagatg gcagtcacct tcattggcaa tagcacagcc atccaggagc
     1321 tcttcaagcg catctcggag cagttcactg ccatgttccg ccggaaggcc ttcctccact
     1381 ggtacacagg cgagggcatg gacgagatgg agttcaccga ggctgagagc aacatgaacg
     1441 acctcgtctc tgagtatcag cagtaccagg atgccaccgc agaagaggag gaggatttcg
     1501 gtgaggaggc cgaagaggag gcctgattgc caactttctt gtacaaagtt ggcattataa
     1561 gaaagcattg cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa aataaaatca
     1621 ttatttgcca tccagctgat atcccctata gtgagtcgta ttacatggtc atagctgttt
     1681 cctggcagct ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa gataaaaata
     1741 tatcatcatg cctcctctag accagccagg acagaaatgc ctcgacttcg ctgctaccca
     1801 aggttgccgg gtgacgcaca ccgtggaaac ggatgaaggc acgaacccag tggacataag
     1861 cctgttcggt tcgtaagctg taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa
     1921 ccttgaccga acgcagcggt ggtaacggcg cagtggcggt tttcatggct tgttatgact
     1981 gtttttttgg ggtacagtct atgcctcggg catccaagca gcaagcgcgt tacgccgtgg
     2041 gtcgatgttt gatgttatgg agcagcaacg atgttacgca gcagggcagt cgccctaaaa
     2101 caaagttaaa cattatgagg gaagcggtga tcgccgaagt atcgactcaa ctatcagagg
     2161 tagttggcgt catcgagcgc catctcgaac cgacgttgct ggccgtacat ttgtacggct
     2221 ccgcagtgga tggcggcctg aagccacaca gtgatattga tttgctggtt acggtgaccg
     2281 taaggcttga tgaaacaacg cggcgagctt tgatcaacga ccttttggaa acttcggctt
     2341 cccctggaga gagcgagatt ctccgcgctg tagaagtcac cattgttgtg cacgacgaca
     2401 tcattccgtg gcgttatcca gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg
     2461 acattcttgc aggtatcttc gagccagcca cgatcgacat tgatctggct atcttgctga
     2521 caaaagcaag agaacatagc gttgccttgg taggtccagc ggcggaggaa ctctttgatc
     2581 cggttcctga acaggatcta tttgaggcgc taaatgaaac cttaacgcta tggaactcgc
     2641 cgcccgactg ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc atttggtaca
     2701 gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca atggagcgcc
     2761 tgccggccca gtatcagccc gtcatacttg aagctagaca ggcttatctt ggacaagaag
     2821 aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt tgtccactac gtgaaaggcg
     2881 agatcaccaa ggtagtcggc aaataaccct cgagccaccc atgaccaaaa tcccttaacg
     2941 tgagttacgc gtcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     3001 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     3061 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     3121 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     3181 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     3241 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     3301 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     3361 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     3421 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     3481 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     3541 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     3601 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     3661 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     3721 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     3781 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     3841 ccgactggaa agcgggcagt gagcgcaacg caattaatac gcgtaccgct agccaggaag
     3901 agtttgtaga aacgcaaaaa ggccatccgt caggatggcc ttctgcttag tttgatgcct
     3961 ggcagtttat ggcgggcgtc ctgcccgcca ccctccgggc cgttgcttca caacgttcaa
     4021 atccgctccc ggcggatttg tcctactcag gagagcgttc accgacaaac aacagataaa
     4081 acgaaaggcc cagtcttccg actgagcctt tcgttttatt tgatgcct
//
