LOCUS       sequence-167366-         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Sensor Plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Mullokandov G, Baccarini A, Ruzo A, Jayaprakash AD, Tung N, Israelow
            B, Evans MJ, Sachidanandam R, Brown BD
  TITLE     High-throughput assessment of microRNA activity and function using 
            microRNA sensor and decoy libraries.
  JOURNAL   Nat Methods. 2012 Jul 1;9(8):840-6. doi: 10.1038/nmeth.2078.
  PUBMED    22751203
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-07-1- 1"; volume: "9"; 
            issue: "8"; pages: "840-6"
COMMENT     Sequence Label: AB.pCCL.sin.cPPT.GFP.miR-151b.sensor.PGK.dNGFR...
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    83..217
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(120..139)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(514..535)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     863..882
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
       61 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataagg
      121 caatagcatc acaaatttca caaataaggc atttttttca ctgcattcta gttttggttt
      181 gtccaaactc atcaatgtat cttatcatgt ctggatctca aatccctcgg aagctgcgcc
      241 tgtcatcgaa ttcctgcagc ccggtgcatg actaagctag taccggttcg aggagctcac
      301 agtcttcgag gagctcacag tcttcgagga gctcacagtc ttcgaggagc tcacagtctt
      361 cgaggagctc acagtcttcg aggagctcac agtctgctac gtcgacagta ctccccagtt
      421 aaggtcggct agctcagtta gcctccccca tctctcgacg cggccgcttt acttgtacag
      481 ctcgtccatg ccgagagtga tcccggcggc ggtcacgaac tccagcagga ccatgtgatc
      541 gcgcttctcg ttggggtctt tgctcagggc ggactgggtg ctcaggtagt ggttgtcggg
      601 cagcagcacg gggccgtcgc cgatgggggt gttctgctgg tagtggtcgg cgagctgcac
      661 gctgccgtcc tcgatgttgt ggcggatctt gaagttcacc ttgatgccgt tcttctgctt
      721 gtcggccatg atatagacgt tgtggctgtt gtagttgtac tccagcttgt gccccaggat
      781 gttgccgtcc tccttgaagt cgatgccctt cagctcgatg cggttcacca gggtgtcgcc
      841 ctcgaacttc acctcggcgc gggtcttgta gttgccgtcg tccttgaa
//
