LOCUS       sequence-167367-         884 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Sensor Plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Mullokandov G, Baccarini A, Ruzo A, Jayaprakash AD, Tung N, Israelow
            B, Evans MJ, Sachidanandam R, Brown BD
  TITLE     High-throughput assessment of microRNA activity and function using 
            microRNA sensor and decoy libraries.
  JOURNAL   Nat Methods. 2012 Jul 1;9(8):840-6. doi: 10.1038/nmeth.2078.
  PUBMED    22751203
REFERENCE   2  (bases 1 to 884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-07-1- 1"; volume: "9"; 
            issue: "8"; pages: "840-6"
COMMENT     Sequence Label: 
            AB.pCCL.sin.cPPT.GFP.miR-512-3p.sensor.PGK.dNGFR.WPRE
FEATURES             Location/Qualifiers
     source          1..884
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    130..264
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(167..186)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(586..607)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 acgagactag cctcgagaga tctgatcata atcagccata ccacatttgt agaggtttta
       61 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
      121 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaggcaa tagcatcaca
      181 aatttcacaa ataaggcatt tttttcactg cattctagtt ttggtttgtc caaactcatc
      241 aatgtatctt atcatgtctg gatctcaaat ccctcggaag ctgcgcctgt catcgaattc
      301 ctgcagcccg gtgcatgact aagctagtac cggtaagtgc tgtcatagct gaggtcaagt
      361 gctgtcatag ctgaggtcaa gtgctgtcat agctgaggtc aagtgctgtc atagctgagg
      421 tcaagtgctg tcatagctga ggtcaagtgc tgtcatagct gaggtcgcta cgtcgaccag
      481 tactccccag ttaaggtcgg ctagctcagt tagcctcccc catctctcga cgcggccgct
      541 ttacttgtac agctcgtcca tgccgagagt gatcccggcg gcggtcacga actccagcag
      601 gaccatgtga tcgcgcttct cgttggggtc tttgctcagg gcggactggg tgctcaggta
      661 gtggttgtcg ggcagcagca cggggccgtc gccgatgggg gtgttctgct ggtagtggtc
      721 ggcgagctgc acgctgccgt cctcgatgtt gtggcggatc ttgaagttca ccttgatgcc
      781 gttcttctgc ttgtcggcca tgatatagac gttgtggctg ttgtagttgt actccagctt
      841 gtgccccagg atgttgccgt cctccttgaa gtcgatgccc ttca
//
