LOCUS       sequence-167377-         895 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Sensor Plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Mullokandov G, Baccarini A, Ruzo A, Jayaprakash AD, Tung N, Israelow
            B, Evans MJ, Sachidanandam R, Brown BD
  TITLE     High-throughput assessment of microRNA activity and function using 
            microRNA sensor and decoy libraries.
  JOURNAL   Nat Methods. 2012 Jul 1;9(8):840-6. doi: 10.1038/nmeth.2078.
  PUBMED    22751203
REFERENCE   2  (bases 1 to 895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-07-1- 1"; volume: "9"; 
            issue: "8"; pages: "840-6"
COMMENT     Sequence Label: 
            AB.pCCL.sin.cPPT.GFP.miR-345-3p.sensor.PGK.dNGFR.WPRE
FEATURES             Location/Qualifiers
     source          1..895
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    153..287
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(190..209)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(609..630)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 aaaaattcaa aattttatcg atcacgagac tagcctcgag agatctgatc ataatcagcc
       61 ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc
      121 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt
      181 acaaataagg caatagcatc acaaatttca caaataaggc atttttttca ctgcattcta
      241 gttttggttt gtccaaactc atcaatgtat cttatcatgt ctggatctca aatccctcgg
      301 aagctgcgcc tgtcatcgaa ttcctgcagc ccggtgcatg actaagctag taccggtgcc
      361 ctgaacgagg ggtctggagg ccctgaacga ggggtctgga ggccctgaac gaggggtctg
      421 gaggccctga acgaggggtc tggaggccct gaacgagggg tctggaggcc ctgaacgagg
      481 ggtctggagg ctacgtcgac cagtactccc cagttaaggt cggctagctc agttagcctc
      541 ccccatctct cgacgcggcc gctttacttg tacagctcgt ccatgccgag agtgatcccg
      601 gcggcggtca cgaactccag caggaccatg tgatcgcgct tctcgttggg gtctttgctc
      661 agggcggact gggtgctcag gtagtggttg tcgggcagca gcacggggcc gtcgccgatg
      721 ggggtgttct gctggtagtg gtcggcgagc tgcacgctgc cgtcctcgat gttgtggcgg
      781 atcttgaagt tcaccttgat gccgttcttc tgcttgtcgg ccatgatata gacgttgtgg
      841 ctgttgtagt tgtactccag cttgtgcccc aggatgttgc cgtcctcctt gaagt
//
