LOCUS       sequence-167380-         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Sensor Plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Mullokandov G, Baccarini A, Ruzo A, Jayaprakash AD, Tung N, Israelow
            B, Evans MJ, Sachidanandam R, Brown BD
  TITLE     High-throughput assessment of microRNA activity and function using 
            microRNA sensor and decoy libraries.
  JOURNAL   Nat Methods. 2012 Jul 1;9(8):840-6. doi: 10.1038/nmeth.2078.
  PUBMED    22751203
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-07-1- 1"; volume: "9"; 
            issue: "8"; pages: "840-6"
COMMENT     Sequence Label: AB.pCCL.sin.cPPT.GFP.miR-564.sensor.PGK.dNGFR....
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    133..267
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(170..189)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(571..592)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atcacgagac tagcctcgag agatctgatc ataatcagcc ataccacatt tgtagaggtt
       61 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
      121 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataagg caatagcatc
      181 acaaatttca caaataaggc atttttttca ctgcattcta gttttggttt gtccaaactc
      241 atcaatgtat cttatcatgt ctggatctca aatccctcgg aagctgcgcc tgtcatcgaa
      301 ttcctgcagc ccggtgcatg actaagctag taccggtagg cacggtgtca gcaggcaggc
      361 acggtgtcag caggcaggca cggtgtcagc aggcaggcac ggtgtcagca ggcaggcacg
      421 gtgtcagcag gcaggcacgg tgtcagcagg cgctacgacg attggtactc cccagttaag
      481 gtcggctagc tcagttagcc tcccccatct ctcgacgcgg ccgctttact tgtacagctc
      541 gtccatgccg agagtgatcc cggcggcggt cacgaactcc agcaggacca tgtgatcgcg
      601 cttctcgttg gggtctttgc tcagggcgga ctgggtgctc aggtagtggt tgtcgggcag
      661 cagcacgggg ccgtcgccga tgggggtgtt ctgctggtag tggtcggcga gctgcacgct
      721 gccgtcctcg atgttgtggc ggatcttgaa gttcaccttg atgccgttct tctgcttgtc
      781 ggccatgata tagacgttgt ggctgttgta gttgtactcc agcttgtgcc
//
