LOCUS       sequence-167382-         645 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Sensor Plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 645)
  AUTHORS   Mullokandov G, Baccarini A, Ruzo A, Jayaprakash AD, Tung N, Israelow
            B, Evans MJ, Sachidanandam R, Brown BD
  TITLE     High-throughput assessment of microRNA activity and function using 
            microRNA sensor and decoy libraries.
  JOURNAL   Nat Methods. 2012 Jul 1;9(8):840-6. doi: 10.1038/nmeth.2078.
  PUBMED    22751203
REFERENCE   2  (bases 1 to 645)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-07-1- 1"; volume: "9"; 
            issue: "8"; pages: "840-6"
COMMENT     Sequence Label: 
            AB.pCCL.sin.cPPT.GFP.miR-181c-5p.sensor.PGK.dNGFR.WPRE
FEATURES             Location/Qualifiers
     source          1..645
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    85..219
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(122..141)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(541..562)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
       61 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
      121 ggcaatagca tcacaaattt cacaaataag gcattttttt cactgcattc tagttttggt
      181 ttgtccaaac tcatcaatgt atcttatcat gtctggatct caaatccctc ggaagctgcg
      241 cctgtcatcg aattcctgca gcccggtgca tgactaagct agtaccggta acattcaacc
      301 tgtcggtgag taacattcaa cctgtcggtg agtaacattc aacctgtcgg tgagtaacat
      361 tcaacctgtc ggtgagtaac attcaacctg tcggtgagta acattcaacc tgtcggtgag
      421 tgctacgtcg accagtactc cccagttaag gtcggctagc tcagttagcc tcccccatct
      481 ctcgacgcgg ccgctttact tgtacagctc gtccatgccg agagtgatcc cggcggcggt
      541 cacgaactcc agcaggacca tgtgatcgcg cttctcgttg gggtctttgc tcagggcgga
      601 ctgggtgctc aggtagtggt tgtcgggcag cagcacgggg ccgtc
//
