LOCUS       sequence-167383-         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Sensor Plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Mullokandov G, Baccarini A, Ruzo A, Jayaprakash AD, Tung N, Israelow
            B, Evans MJ, Sachidanandam R, Brown BD
  TITLE     High-throughput assessment of microRNA activity and function using 
            microRNA sensor and decoy libraries.
  JOURNAL   Nat Methods. 2012 Jul 1;9(8):840-6. doi: 10.1038/nmeth.2078.
  PUBMED    22751203
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-07-1- 1"; volume: "9"; 
            issue: "8"; pages: "840-6"
COMMENT     Sequence Label: 
            AB.pCCL.sin.cPPT.GFP.miR-218-1-3p.sensor.PGK.dNGFR.WPRE
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    137..271
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(174..193)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(593..614)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atcgatcacg agactagcct cgagagatct gatcataatc agccatacca catttgtaga
       61 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
      121 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaggcaatag
      181 catcacaaat ttcacaaata aggcattttt ttcactgcat tctagttttg gtttgtccaa
      241 actcatcaat gtatcttatc atgtctggat ctcaaatccc tcggaagctg cgcctgtcat
      301 cgaattcctg cagcccggtg catgactaag ctagtaccgg tatggttccg tcaagcacca
      361 tggatggttc cgtcaagcac catggatggt tccgtcaagc accatggatg gttccgtcaa
      421 gcaccatgga tggttccgtc aagcaccatg gatggttccg tcaagcacca tgggctacgt
      481 cgaccagtac tccccagtta aggtcggcta gctcagttag cctcccccat ctctcgacgc
      541 ggccgcttta cttgtacagc tcgtccatgc cgagagtgat cccggcggcg gtcacgaact
      601 ccagcaggac catgtgatcg cgcttctcgt tggggtcttt gctcagggcg gactgggtgc
      661 tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt
      721 agtggtcggc gagctgcacg ctgccgtcct cgatgttgtg gcggatcttg aagttcacct
      781 tgatgccgtt cttctgcttg tcggccatga tatagacgtt gtggctgttg tagttgtact
      841 ccagcttgtg cc
//
