LOCUS       sequence-167385-         854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Sensor Plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Mullokandov G, Baccarini A, Ruzo A, Jayaprakash AD, Tung N, Israelow
            B, Evans MJ, Sachidanandam R, Brown BD
  TITLE     High-throughput assessment of microRNA activity and function using 
            microRNA sensor and decoy libraries.
  JOURNAL   Nat Methods. 2012 Jul 1;9(8):840-6. doi: 10.1038/nmeth.2078.
  PUBMED    22751203
REFERENCE   2  (bases 1 to 854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-07-1- 1"; volume: "9"; 
            issue: "8"; pages: "840-6"
COMMENT     Sequence Label: 
            AB.pCCL.sin.cPPT.GFP.miR-409-5p.sensor.PGK.dNGFR.WPRE
FEATURES             Location/Qualifiers
     source          1..854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    135..269
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(172..191)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(591..612)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cgatcacgag actagcctcg agagatctga tcataatcag ccataccaca tttgtagagg
       61 ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg
      121 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa ggcaatagca
      181 tcacaaattt cacaaataag gcattttttt cactgcattc tagttttggt ttgtccaaac
      241 tcatcaatgt atcttatcat gtctggatct caaatccctc ggaagctgcg cctgtcatcg
      301 aattcctgca gcccggtgca tgactaagct agtaccggta ggttacccga gcaactttgc
      361 aaggttaccc gagcaacttt gcaaggttac ccgagcaact ttgcaaggtt acccgagcaa
      421 ctttgcaagg ttacccgagc aactttgcaa ggttacccga gcaactttgc agctacgtcg
      481 accagtactc cccagttaag gtcggctagc tcagttagcc tcccccatct ctcgacgcgg
      541 ccgctttact tgtacagctc gtccatgccg agagtgatcc cggcggcggt cacgaactcc
      601 agcaggacca tgtgatcgcg cttctcgttg gggtctttgc tcagggcgga ctgggtgctc
      661 aggtagtggt tgtcgggcag cagcacgggg ccgtcgccga tgggggtgtt ctgctggtag
      721 tggtcggcga gctgcacgct gccgtcctcg atgttgtggc ggatcttgaa gttcaccttg
      781 atgccgttct tctgcttgtc ggccatgata tagacgttgt ggctgttgta gttgtactcc
      841 agcttgtgcc ccag
//
