LOCUS       sequence-167391-         847 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Sensor Plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Mullokandov G, Baccarini A, Ruzo A, Jayaprakash AD, Tung N, Israelow
            B, Evans MJ, Sachidanandam R, Brown BD
  TITLE     High-throughput assessment of microRNA activity and function using 
            microRNA sensor and decoy libraries.
  JOURNAL   Nat Methods. 2012 Jul 1;9(8):840-6. doi: 10.1038/nmeth.2078.
  PUBMED    22751203
REFERENCE   2  (bases 1 to 847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-07-1- 1"; volume: "9"; 
            issue: "8"; pages: "840-6"
COMMENT     Sequence Label: 
            AB.pCCL.sin.cPPT.GFP.miR-769-5p.sensor.PGK.dNGFR.WPRE
FEATURES             Location/Qualifiers
     source          1..847
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    132..266
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(169..188)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(588..609)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tcacgagact agcctcgaga gatctgatca taatcagcca taccacattt gtagaggttt
       61 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa
      121 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaggc aatagcatca
      181 caaatttcac aaataaggca tttttttcac tgcattctag ttttggtttg tccaaactca
      241 tcaatgtatc ttatcatgtc tggatctcaa atccctcgga agctgcgcct gtcatcgaat
      301 tcctgcagcc cggtgcatga ctaagctagt accggttgag acctctgggt tctgagcttg
      361 agacctctgg gttctgagct tgagacctct gggttctgag cttgagacct ctgggttctg
      421 agcttgagac ctctgggttc tgagcttgag acctctgggt tctgagctgc tacgacgatt
      481 ggtactcccc agttaaggtc ggctagctca gttagcctcc cccatctctc gacgcggccg
      541 ctttacttgt acagctcgtc catgccgaga gtgatcccgg cggcggtcac gaactccagc
      601 aggaccatgt gatcgcgctt ctcgttgggg tctttgctca gggcggactg ggtgctcagg
      661 tagtggttgt cgggcagcag cacggggccg tcgccgatgg gggtgttctg ctggtagtgg
      721 tcggcgagct gcacgctgcc gtcctcgatg ttgtggcgga tcttgaagtt caccttgatg
      781 ccgttcttct gcttgtcggc catgatatag acgttgtggc tgttgtagtt gtactccagc
      841 ttgtgcc
//
