LOCUS       sequence-167399-         845 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Sensor Plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Mullokandov G, Baccarini A, Ruzo A, Jayaprakash AD, Tung N, Israelow
            B, Evans MJ, Sachidanandam R, Brown BD
  TITLE     High-throughput assessment of microRNA activity and function using 
            microRNA sensor and decoy libraries.
  JOURNAL   Nat Methods. 2012 Jul 1;9(8):840-6. doi: 10.1038/nmeth.2078.
  PUBMED    22751203
REFERENCE   2  (bases 1 to 845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-07-1- 1"; volume: "9"; 
            issue: "8"; pages: "840-6"
COMMENT     Sequence Label: 
            AB.pCCL.sin.cPPT.GFP.miR-138-1-3p.sensor.PGK.dNGFR.WPRE
FEATURES             Location/Qualifiers
     source          1..845
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    144..278
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(181..200)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(600..621)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 aaattttatc gatcacgaga ctagcctcga gagatctgat cataatcagc cataccacat
       61 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
      121 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataag
      181 gcaatagcat cacaaatttc acaaataagg catttttttc actgcattct agttttggtt
      241 tgtccaaact catcaatgta tcttatcatg tctggatctc aaatccctcg gaagctgcgc
      301 ctgtcatcga attcctgcag cccggtgcat gactaagcta gtaccggtgc tacttcacaa
      361 caccagggcc gctacttcac aacaccaggg ccgctacttc acaacaccag ggccgctact
      421 tcacaacacc agggccgcta cttcacaaca ccagggccgc tacttcacaa caccagggcc
      481 gctacgtcga ccagtactcc ccagttaagg tcggctagct cagttagcct cccccatctc
      541 tcgacgcggc cgctttactt gtacagctcg tccatgccga gagtgatccc ggcggcggtc
      601 acgaactcca gcaggaccat gtgatcgcgc ttctcgttgg ggtctttgct cagggcggac
      661 tgggtgctca ggtagtggtt gtcgggcagc agcacggggc cgtcgccgat gggggtgttc
      721 tgctggtagt ggtcggcgag ctgcacgctg ccgtcctcga tgttgtggcg gatcttgaag
      781 ttcaccttga tgccgttctt ctgcttgtcg gccatgatat agacgttgtg gctgttgtag
      841 ttgta
//
